About: Selective and facile assay of human immunodeficiency virus protease activity by a novel fluorogenic reaction

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Selective and facile assay of human immunodeficiency virus protease activity by a novel fluorogenic reaction
Creator	Kobayashi, Nobuyuki Kitazato, Kaio Kai, Masaaki Kabashima, Tsutomu Lee, Myung Shibata, Takayuki Tang, Chenhong Yu, Zhiqiang
Source	Elsevier; Medline; PMC
abstract	Abstract A highly selective and facile assay of human immunodeficiency virus protease (HIV-PR) has been required for the screening of medicinal inhibitors and also for classifying the subtypes of HIV in the therapeutic treatment of acquired immune deficiency syndrome (AIDS). This article describes a novel assay method of HIV-PR based on the selective fluorogenic reaction of peptides. A peptide fragment generated from a substrate by the enzymatic digestion with HIV-PR could be selectively quantified by the spectrofluorometric detection after the fluorogenic reaction with catechol in the presence of sodium periodate and sodium borate (pH 7.0). This assay system uses an N-terminal acetyl peptide as the substrate and crude extracts from Escherichia coli expressing recombinant HIV-PR. The activity obtained by the proposed assay correlated with that obtained by a conventional HIV-PR assay based on fluorescence resonance energy transfer detection.
has issue date	2010-02-15(xsd:dateTime)
bibo:doi	10.1016/j.ab.2009.10.030
bibo:pmid	19852926
has license	els-covid
sha1sum (hex)	3601a9f4fd837de3106de6ce3a439916716c1acb
schema:url	https://doi.org/10.1016/j.ab.2009.10.030
resource representing a document's title	Selective and facile assay of human immunodeficiency virus protease activity by a novel fluorogenic reaction
has PubMed Central identifier	PMC7094380
has PubMed identifier	19852926
schema:publication	Analytical Biochemistry
resource representing a document's body	covid:3601a9f4fd837de3106de6ce3a439916716c1acb#body_text
is schema:about of	named entity 'assay' named entity 'fluorogenic' named entity 'NOVEL' named entity 'PROTEASE ACTIVITY' named entity 'SELECTIVE' named entity 'REACTION' named entity 'ASSAY' named entity 'HUMAN IMMUNODEFICIENCY VIRUS' named entity 'Selective' named entity 'human immunodeficiency virus' named entity 'amino acid' named entity '5.5' named entity 'HIV' named entity 'HIV' named entity 'pepstatin' named entity 'Catechol' named entity 'chromosomal DNA' named entity 'spectrofluorometric' named entity 'glycerol' named entity 'reaction mixture' named entity 'reaction mixture' named entity 'Ishikari' named entity 'NaIO' named entity 'HIV' named entity 'yeast extract' named entity 'Tris-HCl' named entity 'HIV' named entity 'correlation coefficient' named entity 'cell lysate' named entity 'amino acid' named entity 'transformed cells' named entity 'FRET' named entity 'peptide' named entity 'NaIO' named entity 'gene' named entity 'fluorogenic' named entity 'colorimetric assay' named entity 'fluorescent' named entity 'enzymatic reaction' named entity 'cell lysate' named entity 'enzyme' named entity 'lysate' named entity 'substrate' named entity 'sodium dodecyl sulfate' named entity 'isoamyl alcohol' named entity 'sulfonic acid' named entity 'HIV' named entity 'N-terminal' named entity 'relative standard deviation' named entity 'protease inhibitors' named entity 'NaIO' named entity 'protease inhibitors' named entity 'HIV' named entity 'acetyl' named entity 'Tokyo' named entity 'fluorescent' named entity 'peptide' named entity 'protease inhibitors' named entity 'transformed cells' named entity 'fluorescence' named entity 'substrate' named entity '5.5' named entity 'alkaline solution' named entity 'HIV' named entity 'plasmid' named entity 'incubation time' named entity 'Dulbecco's modified Eagle's medium' named entity 'enzymatic reaction' named entity 'catechol' named entity 'peptide' named entity 'chloroform' named entity 'borate' named entity 'Gln' named entity 'enzymatic hydrolysis'

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