About: The N-Terminal Domain of Spike Protein Is Not the Enteric Tropism Determinant for Transmissible Gastroenteritis Virus in Piglets

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: The N-Terminal Domain of Spike Protein Is Not the Enteric Tropism Determinant for Transmissible Gastroenteritis Virus in Piglets Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	The N-Terminal Domain of Spike Protein Is Not the Enteric Tropism Determinant for Transmissible Gastroenteritis Virus in Piglets
Creator	Xiao, Shaobo Wang, Gang Peng, Guiqing Shi, Yuejun Shen, Zhou Shi, Jiale Deng, Feng Liang, Rui Liu, Ziwei Fu, Zhen
Source	Medline; PMC
abstract	Transmissible gastroenteritis virus (TGEV) is the etiologic agent of transmissible gastroenteritis in pigs, and the N-terminal domain of TGEV spike protein is generally recognized as both the virulence determinant and enteric tropism determinant. Here, we assembled a full-length infectious cDNA clone of TGEV in a bacterial artificial chromosome. Using a novel approach, the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) systems efficiently and rapidly rescued another recombinant virus with a 224-amino-acid deletion in the N-terminal domain of the TGEV Spike gene (S_NTD224), which is analogous to the N-terminal domain of porcine respiratory coronavirus. S_NTD224 notably affected the TGEV growth kinetics in PK-15 cells but was not essential for recombinant virus survival. In animal experiments with 13 two-day-old piglets, the TGEV recombinant viruses with/without S_NTD224 deletion induced obvious clinical signs and mortality. Together, our results directly demonstrated that S_NTD224 of TGEV mildly influenced TGEV virulence but was not the enteric tropism determinant and provide new insights for the development of a new attenuated vaccine against TGEV. Importantly, the optimized reverse genetics platform used in this study will simplify the construction of mutant infectious clones and help accelerate progress in coronavirus research.
has issue date	2019-03-30(xsd:dateTime)
bibo:doi	10.3390/v11040313
bibo:pmid	30935078
has license	cc-by
sha1sum (hex)	35fd57d62eba3f9b7de6cd27ac5fe63a478a4197
schema:url	https://doi.org/10.3390/v11040313
resource representing a document's title	The N-Terminal Domain of Spike Protein Is Not the Enteric Tropism Determinant for Transmissible Gastroenteritis Virus in Piglets
has PubMed Central identifier	PMC6520731
has PubMed identifier	30935078
schema:publication	Viruses
resource representing a document's body	covid:35fd57d62eba3f9b7de6cd27ac5fe63a478a4197#body_text
is schema:about of	named entity 'assembled' named entity 'enteric' named entity 'Here' named entity 'gastroenteritis' named entity 'piglets' named entity 'deletion' named entity 'DOMAIN' named entity 'PROTEIN ' named entity 'HERE' named entity 'ASSOCIATED' named entity 'RECOMBINANT' named entity 'NOVEL' named entity 'GENE' named entity 'RESEARCH' named entity 'DETERMINANT' named entity 'ATTENUATED VACCINE' named entity 'RESULTS' named entity 'ANIMAL EXPERIMENTS' named entity 'MILDLY' named entity 'MORTALITY' named entity 'HELP' named entity 'INFECTIOUS' named entity 'AFFECTED' named entity 'REGULARLY' named entity 'OBVIOUS' named entity 'TROPISM' named entity 'INSIGHTS' named entity 'PROVIDE' named entity 'OUR' named entity 'PROGRESS' named entity 'PORCINE RESPIRATORY CORONAVIRUS' named entity 'USED' named entity 'SPIKE' named entity 'TRANSMISSIBLE GASTROENTERITIS VIRUS' named entity 'ACCELERATE' named entity 'GASTROENTERITIS' named entity 'CDNA CLONE' named entity 'GROWTH KINETICS' named entity '28S' named entity 'N-TERMINAL ' named entity 'BACTERIAL ARTIFICIAL CHROMOSOME' named entity 'MUTANT' named entity 'ENTERIC' named entity 'REVERSE GENETICS' named entity 'RAPIDLY' named entity 'PLATFORM' named entity 'PROTEIN ' named entity 'INDUCED' named entity 'REPEAT' named entity 'SPIKE PROTEIN' named entity 'CONSTRUCTION' named entity '224' named entity 'USING' named entity 'ETIOLOGIC AGENT' named entity 'SIMPLIFY' named entity 'DETERMINANT' named entity 'DOMAIN' named entity 'TRANSMISSIBLE GASTROENTERITIS VIRUS' named entity 'N-TERMINAL ' named entity 'ENTERIC' named entity 'SHORT' named entity 'APPROACH' named entity 'DIRECTLY' named entity 'CLONES' named entity 'STUDY' named entity 'CELLS' named entity 'DEVELOPMENT' named entity 'RECOMBINANT VIRUS' named entity 'NEW' named entity 'GENERALLY' named entity 'CORONAVIRUS' named entity 'AMINO-ACID'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2025 OpenLink Software