About: Discovery and validation of serum biomarkers expressed over the first twelve weeks of Fasciola hepatica infection in sheep

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Discovery and validation of serum biomarkers expressed over the first twelve weeks of Fasciola hepatica infection in sheep Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Discovery and validation of serum biomarkers expressed over the first twelve weeks of Fasciola hepatica infection in sheep
Creator	Ward, B Acosta, D Bennett, H Carmona, C Gibbs, B Ndao, M Rioux, M.-C Spithill, T
Source	Elsevier; Medline; PMC
abstract	Abstract Serum biomarkers associated with Fasciola hepatica infection of Corriedale sheep were analysed during the first 12 weeks of infection using surface-enhanced laser desorption ionisation time of flight mass spectrometry (SELDI-TOF MS). In the discovery phase of analysis, pooled sera collected at week 0 and at each week p.i. to week 12 were fractionated by anion-exchange chromatography and the protein mass fingerprints obtained in individual fractions were in the M/z range 1.5–150kDa. A total of 2302 protein clusters (peaks) were identified that varied between time-points following infection with peaks increasing or decreasing in intensity, or showing transient variation in intensity, during the 12 weeks of parasite challenge. In the validation phase, candidate biomarkers in sera of individual sheep at weeks 3 and 9 p.i. were analysed, identifying 100 protein peaks, many of which are small peptides <10kDa in size: 54% of these peaks were up-regulated in intensity at week 3 or 9 p.i. Twenty-six biomarkers were chosen for further study, ranging in size from 1832 to 89,823Da: six biomarkers were up-regulated at weeks 3 and 9 p.i., 16 biomarkers were up-regulated only at week 9 p.i. and four biomarkers were down-regulated at week 9 p.i. Two biomarkers up-regulated at week 9 were identified as transferrin (77.2kDa) and Apolipoprotein A-IV (44.3kDa), respectively. The results show that the interaction between the host and F. hepatica is complex, with changes in biomarker patterns beginning within 3 weeks of infection and either persisting to weeks 9–12 or showing transient changes during infection. Identification of biomarkers expressed during ovine fasciolosis may provide insights into mechanisms of pathogenesis and immunity to Fasciola and may assist in the rational development and delivery of vaccines.
has issue date	2008-01-31(xsd:dateTime)
bibo:doi	10.1016/j.ijpara.2007.07.017
bibo:pmid	17888928
has license	els-covid
sha1sum (hex)	35f74dd851dc3b8e611c187480bcea891d8966c5
schema:url	https://doi.org/10.1016/j.ijpara.2007.07.017
resource representing a document's title	Discovery and validation of serum biomarkers expressed over the first twelve weeks of Fasciola hepatica infection in sheep
has PubMed Central identifier	PMC7094367
has PubMed identifier	17888928
schema:publication	International Journal for Parasitology
resource representing a document's body	covid:35f74dd851dc3b8e611c187480bcea891d8966c5#body_text
is schema:about of	named entity 'size' named entity 'clusters' named entity 'infection' named entity 'fractionated' named entity 'sheep' named entity 'time' named entity 'complex' named entity 'fingerprints' named entity 'sheep' named entity 'KDA' named entity 'INFECTION' named entity 'VARIATION' named entity 'BIOMARKERS' named entity 'CHANGES' named entity 'CHALLENGE' named entity 'DECREASING' named entity 'BIOMARKER' named entity 'TOF MS' named entity 'STUDY' named entity 'INDIVIDUAL' named entity 'UP-REGULATED' named entity 'PROVIDE' named entity 'ENHANCED' named entity 'APOLIPOPROTEIN A-IV' named entity 'DOWN-REGULATED' named entity 'RANGE' named entity 'POOLED' named entity 'IDENTIFIED' named entity 'USING' named entity 'FASCIOLA HEPATICA INFECTION' named entity 'FRACTIONS' named entity 'INTERACTION' named entity 'HOST' named entity 'COMPLEX' named entity 'MASS' named entity 'COLLECTED' named entity 'TIME' named entity 'POINTS' named entity 'PROTEIN ' named entity 'FASCIOLA HEPATICA INFECTION' named entity 'CLUSTERS' named entity 'FASCIOLOSIS' named entity 'WEEKS' named entity 'SHEEP' named entity 'SERUM BIOMARKERS' named entity 'EXPRESSED' named entity '150' named entity 'VALIDATION' named entity 'PHASE' named entity 'EITHER' named entity '1.5' named entity 'ANION-EXCHANGE CHROMATOGRAPHY' named entity 'CHOSEN' named entity 'WEEKS' named entity 'FASCIOLA' named entity 'DEVELOPMENT' named entity 'OVINE' named entity 'SMALL' named entity 'WEEK' named entity 'FRACTIONATED' named entity 'IMMUNITY' named entity 'TWENTY-SIX' named entity 'INSIGHTS' named entity '3C10' named entity 'INCREASING' named entity '100' named entity 'THESE' named entity 'IDENTIFYING' named entity 'IDENTIFICATION' named entity 'PATTERNS' named entity '12 WEEKS' named entity 'RESULTS' named entity 'DELIVERY' named entity 'TIME OF FLIGHT MASS SPECTROMETRY'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software