About: Use of a Safe, Reproducible, and Rapid Aerosol Delivery Method to Study Infection by Burkholderia pseudomallei and Burkholderia mallei in Mice

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About: Use of a Safe, Reproducible, and Rapid Aerosol Delivery Method to Study Infection by Burkholderia pseudomallei and Burkholderia mallei in Mice Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Use of a Safe, Reproducible, and Rapid Aerosol Delivery Method to Study Infection by Burkholderia pseudomallei and Burkholderia mallei in Mice
Creator	Gao, Xiudan Michel, Frank Hogan, Robert Lafontaine, Eric Shaffer, Teresa Zimmerman, Shawn
Source	Medline; PMC
abstract	Burkholderia pseudomallei, the etiologic agent of melioidosis, is a saprophytic bacterium readily isolated from wet soils of countries bordering the equator. Burkholderia mallei is a host-adapted clone of B. pseudomallei that does not persist outside of its equine reservoir and causes the zoonosis glanders, which is endemic in Asia, Africa, the Middle East and South America. Infection by these organisms typically occurs via percutaneous inoculation or inhalation of aerosols, and the most common manifestation is severe pneumonia leading to fatal bacteremia. Glanders and melioidosis are difficult to diagnose and require prolonged antibiotic therapy with low success rates. There are no vaccines available to protect against either Burkholderia species, and there is concern regarding their use as biological warfare agents given that B. mallei has previously been utilized in this manner. Hence, experiments were performed to establish a mouse model of aerosol infection to study the organisms and develop countermeasures. Using a hand-held aerosolizer, BALB/c mice were inoculated intratracheally with strains B. pseudomallei 1026b and B. mallei ATCC23344 and growth of the agents in the lungs, as well as dissemination to the spleen, were examined. Mice infected with 10(2), 10(3) and 10(4) organisms were unable to control growth of B. mallei in the lungs and bacteria rapidly disseminated to the spleen. Though similar results were observed in mice inoculated with 10(3) and 10(4) B. pseudomallei cells, animals infected with 10(2) organisms controlled bacterial replication in the lungs, dissemination to the spleen, and the extent of bacteremia. Analysis of sera from mice surviving acute infection revealed that animals produced antibodies against antigens known to be targets of the immune response in humans. Taken together, these data show that small volume aerosol inoculation of mice results in acute disease, dose-dependent chronic infection, and immune responses that correlate with those seen in human infections.
has issue date	2013-10-02(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0076804
bibo:pmid	24098563
has license	cc-by
sha1sum (hex)	33b590b2a517896a894c71c1c0b86c593804c034
schema:url	https://doi.org/10.1371/journal.pone.0076804
resource representing a document's title	Use of a Safe, Reproducible, and Rapid Aerosol Delivery Method to Study Infection by Burkholderia pseudomallei and Burkholderia mallei in Mice
has PubMed Central identifier	PMC3788738
has PubMed identifier	24098563
schema:publication	PLoS One
resource representing a document's body	covid:33b590b2a517896a894c71c1c0b86c593804c034#body_text
is schema:about of	named entity 'BURKHOLDERIA PSEUDOMALLEI' named entity 'Middle East' named entity 'inoculation' named entity 'Burkholderia mallei' named entity 'equine' named entity 'STUDY' named entity 'ENDEMIC' named entity 'SEVERE PNEUMONIA' named entity 'ZOONOSIS' named entity 'PROLONGED' named entity 'PERFORMED' named entity 'ESTABLISH' named entity 'AEROSOLIZER' named entity 'PREVIOUSLY' named entity 'MANIFESTATION' named entity 'SPECIES' named entity 'USING' named entity 'LOW' named entity 'EXPERIMENTS' named entity 'MOUSE MODEL' named entity 'ISOLATED' named entity 'AEROSOL' named entity 'THEIR' named entity 'CLONE' named entity 'EQUINE' named entity 'BURKHOLDERIA PSEUDOMALLEI' named entity 'SUCCESS' named entity 'STUDY' named entity 'ANTIBIOTIC THERAPY' named entity 'SOILS' named entity 'ASIA' named entity 'CAUSES' named entity 'AFRICA' named entity 'FATAL' named entity 'GIVEN' named entity 'RESERVOIR' named entity 'BACTERIUM' named entity 'GLANDERS' named entity 'GLANDERS AND MELIOIDOSIS' named entity 'BACTEREMIA' named entity 'MIDDLE EAST' named entity 'A MOUSE' named entity 'THESE' named entity 'COMMON' named entity 'CONCERN' named entity 'ADAPTED' named entity 'ORGANISMS' named entity 'HOST' named entity 'USE' named entity 'SOUTH AMERICA' named entity 'STRAINS' named entity 'BIOLOGICAL WARFARE AGENTS' named entity 'INHALATION' named entity 'DIAGNOSE' named entity 'RATES' named entity 'AVAILABLE' named entity 'EQUATOR' named entity 'MODEL OF' named entity 'MELIOIDOSIS' named entity 'INFECTION' named entity 'USE OF' named entity 'BURKHOLDERIA MALLEI' named entity 'DELIVERY METHOD' named entity 'RAPID' named entity 'AEROSOL' named entity 'SAFE' named entity 'IS A' named entity 'EITHER' named entity 'WET' named entity 'BORDERING' named entity 'DIFFICULT' named entity 'PERCUTANEOUS' named entity 'BURKHOLDERIA' named entity 'INOCULATION' named entity 'AEROSOLS' named entity 'BURKHOLDERIA MALLEI'

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