About: SARS-CoV-2 Nsp1 binds ribosomal mRNA channel to inhibit translation

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About: SARS-CoV-2 Nsp1 binds ribosomal mRNA channel to inhibit translation Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	SARS-CoV-2 Nsp1 binds ribosomal mRNA channel to inhibit translation
Creator	Thiel, Volker Ban, Nenad Gurzeler, Lukas-Adrian Karousis, Evangelos Mühlemann, Oliver Echeverria, Blanca Jomaa, Ahmad Leibundgut, Marc Scaiola, Alain Schubert, Katharina
Source	BioRxiv
abstract	The non-structural protein 1 (Nsp1), also referred to as the host shutoff factor, is the first viral protein that is synthesized in SARS-CoV-2 infected human cells to suppress host innate immune functions1,2. By combining cryo-electron microscopy and biochemical experiments, we show that SARS-CoV-2 Nsp1 binds to the human 40S subunit in ribosomal complexes including the 43S pre-initiation complex. The protein inserts its C-terminal domain at the entrance to the mRNA channel where it interferes with mRNA binding. We observe potent translation inhibition in the presence of Nsp1 in lysates from human cells. Based on the high-resolution structure of the 40S-Nsp1 complex, we identify residues of Nsp1 crucial for mediating translation inhibition. We further show that the full-length 5’ untranslated region of the genomic viral mRNA stimulates translation in vitro, suggesting that SARS-CoV-2 combines inhibition of translation by Nsp1 with efficient translation of the viral mRNA to achieve expression of viral genes3.
has issue date	2020-07-07(xsd:dateTime)
bibo:doi	10.1101/2020.07.07.191676
has license	biorxiv
sha1sum (hex)	32c8941fd2c9c6d6e73934dca2012769c542bd49
schema:url	https://doi.org/10.1101/2020.07.07.191676
resource representing a document's title	SARS-CoV-2 Nsp1 binds ribosomal mRNA channel to inhibit translation
schema:publication	bioRxiv
resource representing a document's body	covid:32c8941fd2c9c6d6e73934dca2012769c542bd49#body_text
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