About: Host protective ASP-based vaccine against the parasitic nematode Ostertagia ostertagi triggers NK cell activation and mixed IgG1-IgG2 response

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About: Host protective ASP-based vaccine against the parasitic nematode Ostertagia ostertagi triggers NK cell activation and mixed IgG1-IgG2 response Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Host protective ASP-based vaccine against the parasitic nematode Ostertagia ostertagi triggers NK cell activation and mixed IgG1-IgG2 response
Creator	Claerebout, Edwin Geldhof, Peter Borloo, Jimmy González-Hernández, Ana Leclercq, Georges Paerewijck, Oonagh Peelaers, Iris Van Coppernolle, Stefanie Van Meulder, Frederik
Source	PMC
abstract	The mucus-dwelling parasite Ostertagia ostertagi is one of the most important gastrointestinal nematodes in cattle. Our group has previously demonstrated the protective capacity of a vaccine against this parasite based on a native activation-associated secreted protein ASP1 (nASP) in combination with the saponin adjuvant QuilA. The aim of the current study was to analyse the effect of both antigen and adjuvant on the cellular and humoral vaccine-induced immune responses by comparing the native ASP to a recombinant version expressed in Pichia pastoris (pASP) and replacing QuilA by Al(OH)(3). Immunization of cattle with the protective nASP+QuilA vaccine was associated with antigen-induced proliferation of natural killer (NK) cells combined with IFN-γ secretion and the induction of a mixed IgG1/IgG2 antibody response. ASP-specific activation and proliferation of NK cells was also observed in mice following the same vaccination regime. Replacing QuilA by Al(OH)(3) or nASP by pASP significantly decreased the capacity of the vaccines to trigger both NK cell activation and antibody responses and failed to induce protection against a challenge infection. Reduction of the structurally anchoring disulphide bonds of the nASP completely abolished its ability to induce NK cell activation and antibody responses, highlighting the importance of protein conformation for the immunostimulatory activity.
has issue date	2016-07-11(xsd:dateTime)
bibo:doi	10.1038/srep29496
bibo:pmid	27403891
has license	cc-by
sha1sum (hex)	31c0ee2b6dff1699d251316667c0b56c7b8f09a3
schema:url	https://doi.org/10.1038/srep29496
resource representing a document's title	Host protective ASP-based vaccine against the parasitic nematode Ostertagia ostertagi triggers NK cell activation and mixed IgG1-IgG2 response
has PubMed Central identifier	PMC4941725
has PubMed identifier	27403891
schema:publication	Sci Rep
resource representing a document's body	covid:31c0ee2b6dff1699d251316667c0b56c7b8f09a3#body_text
is schema:about of	named entity 'observed' named entity 'vaccines' named entity 'activation' named entity 'analyse' named entity 'activity' named entity 'proliferation' named entity 'cattle' named entity 'triggers' covid:arg/31c0ee2b6dff1699d251316667c0b56c7b8f09a3 named entity 'natural killer (NK) cells' named entity 'NK cells' named entity 'structurally' named entity 'infection' named entity 'parasite' named entity 'completely' named entity 'NK cells' named entity 'CD19' named entity 'pASP' named entity 'DTT' named entity 'worm' named entity 'IgG1' named entity 'PBS' named entity 'MNCs' named entity 'NK cells' named entity 'demonstrated' named entity 'immune responses' named entity 'IFN-γ' named entity 'Ostertagia ostertagi' named entity 'vaccine' named entity 'parasite' named entity 'gastrointestinal' named entity 'cattle' named entity 'vaccine' named entity 'adjuvant' named entity 'antigen' named entity 'disulphide bonds' named entity 'immunostimulatory' named entity 'mice' named entity 'pASP' named entity 'vaccination' named entity 'Flow Cytometry' named entity 'IgG1' named entity 'Optical density' named entity 'statistical significance' named entity 'BD Biosciences' named entity 'IFNγ' named entity 'muscle' named entity 'parasite' named entity 'IgG2' named entity 'SEM' named entity 'NK cells' named entity 'IgG2a' named entity 'Sigma-Aldrich' named entity 'IgG1' named entity 'IgG2' named entity 'CD8a' named entity 'worm' named entity 'antigen' named entity 'lymph nodes' named entity 'pathogen' named entity 'antigen' named entity 'IgG2b' named entity 'Pichia pastoris' named entity 'cellular immune response' named entity 'immunomodulatory' named entity 'abomasal' named entity 'O. ostertagi' named entity 'pASP' named entity 'vaccine' named entity 'PBMCs' named entity 'fetal calf serum' named entity 'IgG2b' named entity 'cytokines'

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