About: Multiprotein Complex Production in Insect Cells by Using Polyproteins

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Multiprotein Complex Production in Insect Cells by Using Polyproteins
Creator	Berger, Imre Bieniossek, Christoph Trowitzsch, Simon Nie, Yan Bellon-Echeverria, Itxaso
topic	covid:30d9a7e47d4792dba54b0b71643ac5da299f597f#this
Source	Medline; PMC
abstract	A powerful approach utilizing polyproteins for balancing stoichiometry of recombinant multiprotein complexes overproduced in baculovirus expression vector systems (BEVS) is described. This procedure has been implemented here in the MultiBac system but can also be directly adapted to all commonly used BEVS. The protocol details the design principles of polyprotein-expressing DNA constructs, the generation of composite baculovirus for polyprotein production, and the expression and in vivo processing of polyproteins in baculovirus infected insect cells.
has issue date	2013-07-27(xsd:dateTime)
bibo:doi	10.1007/978-1-62703-691-7_8
bibo:pmid	24203328
has license	no-cc
sha1sum (hex)	30d9a7e47d4792dba54b0b71643ac5da299f597f
schema:url	https://doi.org/10.1007/978-1-62703-691-7_8
resource representing a document's title	Multiprotein Complex Production in Insect Cells by Using Polyproteins
has PubMed Central identifier	PMC7120617
has PubMed identifier	24203328
schema:publication	Structural Genomics
resource representing a document's body	covid:30d9a7e47d4792dba54b0b71643ac5da299f597f#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/30d9a7e47d4792dba54b0b71643ac5da299f597f
is schema:about of	named entity 'RECOMBINANT' named entity 'BACULOVIRUS' named entity 'COMPOSITE' named entity 'HERE' named entity 'USED' named entity 'STOICHIOMETRY' named entity 'DESIGN' named entity 'GENERATION' named entity 'IN VIVO' named entity 'INSECT' named entity 'baculovirus' named entity 'powerful' named entity 'CHAPTER' named entity 'INSECT' named entity 'PRODUCTION' named entity 'MULTIPROTEIN COMPLEXES' named entity 'BUT' named entity 'ADAPTED' named entity 'PROCEDURE' named entity 'INFECTED' named entity 'CELLS' named entity 'PROCESSING' named entity 'BALANCING' named entity 'PRODUCTION' named entity 'MULTIPROTEIN COMPLEX' named entity 'USING' named entity 'CELLS' named entity 'POLYPROTEINS' named entity 'POLYPROTEINS' named entity 'DESCRIBED' named entity 'EXPRESSION' named entity 'EXPRESSION VECTOR' named entity 'POWERFUL' named entity 'POLYPROTEIN' named entity 'DNA' named entity 'DETAILS' named entity 'SYSTEMS' named entity 'DIRECTLY' named entity 'PROTOCOL' named entity 'SYSTEM' named entity 'IMPLEMENTED' named entity 'APPROACH' named entity 'adapted' named entity 'details' named entity 'polyprotein' named entity 'baculovirus' named entity 'baculovirus' named entity 'Multiprotein complexes' named entity 'gel extraction' named entity 'polyprotein' named entity 'plasmid' named entity 'origin of replication' named entity 'Multiprotein complexes' named entity 'ColE1' named entity 'Gene synthesis' named entity 'general transcription factor' named entity 'open reading frame' named entity 'TEV' named entity 'DNA cloning' named entity 'protease' named entity 'protein' named entity 'gene' named entity 'genomes' named entity 'polyprotein' named entity 'TEV' named entity 'protease' named entity 'spectrophotometrically' named entity 'transposition' named entity 'polyhedrin' named entity 'plasmid' named entity 'Heterologous' named entity 'expression cassette' named entity 'recombinant' named entity 'baculovirus' named entity 'baculovirus'

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