About: Graphene field-effect transistor biosensor for detection of biotin with ultrahigh sensitivity and specificity

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About: Graphene field-effect transistor biosensor for detection of biotin with ultrahigh sensitivity and specificity Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Graphene field-effect transistor biosensor for detection of biotin with ultrahigh sensitivity and specificity
Creator	Hossain, Zakir Ichige, Ryo Kitada, Shunya Kobayashi, Haruo Kuwana, Anna Shimizu, Natsuhiko Shinozuka, Kazuo Suzuki, Takaaki Wang, Shiyu
Source	Elsevier; Medline; PMC
abstract	Because avidin and biotin molecules exhibit the most specific and strongest non-covalent interaction, avidin-biotin technology is widely used in ELISA (enzyme-linked immunosorbent assay) kits for the detection of different bio-macromolecules linked to different diseases including cancer and influenza. Combining the outstanding electrical conductivity (200,000 cm(2)V(-1)s(-1)) of graphene with the unique avidin and biotin interaction, we demonstrate a novel graphene field-effect transistor (GFET) biosensor for the quantitative detection of bio-macromolecules. The GFET consists of six pairs of interdigital Cr/Au electrodes supported on Si/SiO(2) substrate with an avidin immobilized single layer graphene channel as the sensing platform. By monitoring the real time current change upon the addition of biotin solution in bovine serum albumin (BSA) in the silicone pool preformed onto the GFET, the lowest detectable biotin concentration is estimated to be 90 fg/ml (0.37 pM). The specificity of the GFET is confirmed both by controlled and real sample measurements From the magnitude of current change upon the addition of different concentrations of biotin solutions, the dissociation constant K(d) is estimated to be 1.6 × 10(-11) M. Since biotin is capable of conjugating with proteins, nucleotides and other bio-macromolecules without altering their properties, the present GFET sensor with its ultra-high sensitivity (0.37 pM) and specificity can be tailored to the rapid point-of-care detection of different types of desired biomolecules at very low concentration level through biotinylation as well as the exogenous biotin in blood serum.
has issue date	2020-06-04(xsd:dateTime)
bibo:doi	10.1016/j.bios.2020.112363
bibo:pmid	32729495
has license	no-cc
sha1sum (hex)	2fc4134eee7d705343f0d0fbf0b9df4094a868b5
schema:url	https://doi.org/10.1016/j.bios.2020.112363
resource representing a document's title	Graphene field-effect transistor biosensor for detection of biotin with ultrahigh sensitivity and specificity
has PubMed Central identifier	PMC7272179
has PubMed identifier	32729495
schema:publication	Biosens Bioelectron
resource representing a document's body	covid:2fc4134eee7d705343f0d0fbf0b9df4094a868b5#body_text
is schema:about of	named entity 'biotin' named entity 'field-effect transistor' named entity 'Graphene' named entity 'sensitivity and specificity' named entity 'biosensor' named entity 'Graphene' named entity 'biotin' named entity 'field-effect transistor' named entity 'SENSITIVITY AND SPECIFICITY' named entity 'FIELD' named entity 'BIOSENSOR' named entity 'TRANSISTOR' named entity 'JOURNAL ' named entity 'DETECTION' named entity 'detection' named entity 'sensitivity' named entity 'Journal' named entity 'biosensor' named entity 'specificity' named entity 'GRAPHENE' named entity 'PROOF' named entity 'BIOTIN' named entity 'PRE' named entity 'EFFECT'
is part of of	Graphene field-effect transistor biosensor for detection of biotin with ultrahigh sensitivity and specificity covid:2fc4134eee7d705343f0d0fbf0b9df4094a868b5#body_text Because avidin and biotin molecules exhibit the most specific and strongest non-covalent interaction, avidin-biotin technology is widely used in ELISA (enzyme-linked immunosorbent assay) kits for the detection of different bio-macromolecules linked to different diseases including cancer and influenza. Combining the outstanding electrical conductivity (200,000 cm(2)V(-1)s(-1)) of graphene with the unique avidin and biotin interaction, we demonstrate a novel graphene field-effect transistor (GFET) biosensor for the quantitative detection of bio-macromolecules. The GFET consists of six pairs of interdigital Cr/Au electrodes supported on Si/SiO(2) substrate with an avidin immobilized single layer graphene channel as the sensing platform. By monitoring the real time current change upon the addition of biotin solution in bovine serum albumin (BSA) in the silicone pool preformed onto the GFET, the lowest detectable biotin concentration is estimated to be 90 fg/ml (0.37 pM). The specificity of the GFET is confirmed both by controlled and real sample measurements From the magnitude of current change upon the addition of different concentrations of biotin solutions, the dissociation constant K(d) is estimated to be 1.6 × 10(-11) M. Since biotin is capable of conjugating with proteins, nucleotides and other bio-macromolecules without altering their properties, the present GFET sensor with its ultra-high sensitivity (0.37 pM) and specificity can be tailored to the rapid point-of-care detection of different types of desired biomolecules at very low concentration level through biotinylation as well as the exogenous biotin in blood serum.

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