About: Novel Method for Isolation of Murine Clara Cell Secretory Protein-Expressing Cells with Traces of Stemness

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About: Novel Method for Isolation of Murine Clara Cell Secretory Protein-Expressing Cells with Traces of Stemness Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Novel Method for Isolation of Murine Clara Cell Secretory Protein-Expressing Cells with Traces of Stemness
Creator	Yan, Yang Jensen-Taubman, K Keefe, X-Y
Source	PMC
abstract	Clara cells are non-ciliated, secretory bronchiolar epithelial cells that serve to detoxify harmful inhaled substances. Clara cells also function as stem/progenitor cells for repair in the bronchioles. Clara cell secretory protein (CCSP) is specifically expressed in pulmonary Clara cells and is widely used as a Clara cell marker. In addition CCSP promoter is commonly used to direct gene expression into the lung in transgenic models. The discovery of CCSP immunoreactivity in plasma membranes of airway lining cells prompted us to explore the possibility of enriching Clara cells by flow cytometry. We established a novel and simple method for the isolation of CCSP-expressing cell Clara cells using a combination of mechanical and enzymatic dissociation followed by flow cytometry sorting technology. We showed that ∼25% of dissociated cells from whole lung expressed CCSP. In the resulting preparation, up to 98% of cells expressed CCSP. Notably, we found that several common stem cell markers including CD44, CD133, Sca-1 and Sox2 were expressed in CCSP(+) cells. Moreover, CCSP(+) cells were able to form spheroid colonies in vitro with 0.97‰ efficiency. Parallel studies in vivo confirmed that a small population of CCSP(−)expressing cells in mouse airways also demonstrates stem cell-like properties such as label retention and harboring rare bronchioalveolar stem cells (BASCs) in terminal bronchioles (TBs). We conclude that CCSP(+) cells exhibit a number of stem cell-like features including stem cell marker expression, bronchosphere colony formation and self-renewal ability. Clara cell isolation by flow cytometry sorting is a useful method for investigating the function of primary Clara cells in stem cell research and mouse models.
has issue date	2012-08-16(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0043008
bibo:pmid	22916196
has license	cc0
sha1sum (hex)	2e5bfe1233f49b63cd653c03a695b24f42adaedd
schema:url	https://doi.org/10.1371/journal.pone.0043008
resource representing a document's title	Novel Method for Isolation of Murine Clara Cell Secretory Protein-Expressing Cells with Traces of Stemness
has PubMed Central identifier	PMC3420884
has PubMed identifier	22916196
schema:publication	PLoS One
resource representing a document's body	covid:2e5bfe1233f49b63cd653c03a695b24f42adaedd#body_text
is schema:about of	named entity 'stem' named entity 'plasma' named entity 'expression' named entity 'number' named entity 'secretory protein' named entity 'TO FORM' named entity 'FLOW CYTOMETRY' named entity 'INVESTIGATING' named entity 'SECRETORY' named entity 'UP TO' named entity 'SOX2' named entity 'LINING' named entity 'STEM CELL MARKER' named entity 'ESTABLISHED' named entity 'CELL MARKER' named entity 'MOUSE' named entity 'GENE EXPRESSION' named entity 'BRONCHIOALVEOLAR STEM CELLS' named entity 'CD133' named entity 'POSSIBILITY OF' named entity 'COMBINATION' named entity 'CLARA' named entity 'TRANSGENIC' named entity 'RETENTION' named entity 'METHOD' named entity 'FUNCTION' named entity 'PREPARATION' named entity 'SIMPLE' named entity 'FEATURES' named entity 'ENZYMATIC DISSOCIATION' named entity 'INCLUDING' named entity 'PROMPTED' named entity 'MODELS' named entity 'CONFIRMED' named entity 'NON-' named entity 'MARKERS' named entity 'COLONIES' named entity 'IS A' named entity 'RARE' named entity 'TECHNOLOGY' named entity 'IN VIVO' named entity 'DISSOCIATED' named entity 'AIRWAY' named entity 'CELL' named entity 'USED' named entity 'MECHANICAL' named entity 'LIKE' named entity 'HARMFUL' named entity 'CD44' named entity 'STEM' named entity 'LUNG' named entity 'IMMUNOREACTIVITY' named entity 'PROTEIN ' named entity 'PROMOTER' named entity 'ABILITY' named entity 'ADDITION' named entity 'REPAIR' named entity 'BRONCHIOLAR' named entity 'CLARA CELL SECRETORY PROTEIN' named entity 'COMMON' named entity 'STEMNESS' named entity 'CELL ISOLATION' named entity 'TO DIRECT' named entity 'SMALL' named entity 'CONCLUDE' named entity 'CILIATED' named entity 'AIRWAYS' named entity 'PROPERTIES' named entity 'USING' named entity 'MURINE' named entity 'CLARA CELL SECRETORY PROTEIN' named entity 'NUMBER OF' named entity 'HARBORING' named entity 'CELLS' named entity 'ISOLATION' named entity 'ISOLATION' named entity 'CELLS' named entity 'STEM CELL' named entity 'IN VITRO'

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