About: Evaluation of a multiplex ligation-dependent probe amplification assay for the detection of respiratory pathogens in oncological patients

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About: Evaluation of a multiplex ligation-dependent probe amplification assay for the detection of respiratory pathogens in oncological patients Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Evaluation of a multiplex ligation-dependent probe amplification assay for the detection of respiratory pathogens in oncological patients
Creator	Apfalter, Petra Berning, Lucia Kerschner, Heidrun Luger, Christoph Machherndl-Spandl, Sigrid Simon, Benedikt Aberle, Stephan
Source	Elsevier; Medline; PMC
abstract	BACKGROUND: Respiratory tract infections are widespread and may cause significant morbidity and mortality in immunosuppressed populations such as oncological patients. OBJECTIVES: The RealAccurate Respiratory RT PCR Kit covering 14 respiratory viruses was compared to the RespiFinder Smart22, a broad-spectrum multiplex ligation-dependent probe amplification (MLPA) test, targeting 22 viral and bacterial respiratory pathogens. STUDY DESIGN: After verification of its analytical performance, the clinical performance of the RespiFinder Smart22 was evaluated by re-analysis of 96 respiratory samples from oncological patients. Additionally, the time to result (TTR) of both methods was compared. RESULTS: The analytical performance of the RespiFinder Smart22 fulfilled all previously specified criteria. Concordant results in both assays were achieved in 74.0% of all clinical specimens and in 91.2% when only positive results were taken into account. The RespiFinder Smart22 yielded additional results in a total of 22 (22.9% of 96) samples due to higher test sensitivity and a broader, highly multiplexed spectrum of pathogens. The TTR of a typical routine test consisting of three samples were 206 and 356 min for the RealAccurate Respiratory RT PCR Kit and the RespiFinder Smart22, respectively. However, hands-on time was reduced by 59.0% applying the MLPA method. CONCLUSIONS: In our hands, the RespiFinder Smart22 showed excellent analytical performance while hands-on time was halved in comparison to the RT PCR method. Regarding the clinical evaluation, the MLPA method provided additional results in 22.9% (22/96) of specimens due to its comprehensive format, higher test sensitivity and the capability to detect 22 pathogens compared to 14 with the RealAccurate Respiratory RT PCR Kit.
has issue date	2014-03-06(xsd:dateTime)
bibo:doi	10.1016/j.jcv.2014.02.010
bibo:pmid	24684925
has license	no-cc
sha1sum (hex)	2d9ea8d268e89f7b19e3f1782f955933195283e3
schema:url	https://doi.org/10.1016/j.jcv.2014.02.010
resource representing a document's title	Evaluation of a multiplex ligation-dependent probe amplification assay for the detection of respiratory pathogens in oncological patients
has PubMed Central identifier	PMC7106480
has PubMed identifier	24684925
schema:publication	J Clin Virol
resource representing a document's body	covid:2d9ea8d268e89f7b19e3f1782f955933195283e3#body_text
is schema:about of	named entity 'test sensitivity' named entity 'performance' named entity 'MLPA' named entity 'compared' named entity 'targeting' named entity 'highly' named entity 'CAPABILITY' named entity 'TAKEN' named entity 'MULTIPLEX LIGATION-DEPENDENT PROBE AMPLIFICATION ' named entity 'MIN' named entity 'BROAD' named entity 'TEST SENSITIVITY' named entity 'OUR' named entity 'SPECIFIED' named entity 'COVERING' named entity 'FORMAT' named entity 'PROVIDED' named entity 'TARGETING' named entity 'RESULTS' named entity 'TOTAL' named entity 'RESPIRATORY VIRUSES' named entity 'RT PCR' named entity 'IMMUNOSUPPRESSED' named entity 'ACCOUNT' named entity 'HIGHER' named entity 'CONCLUSIONS' named entity 'CRITERIA' named entity 'MULTIPLEX LIGATION-DEPENDENT PROBE AMPLIFICATION ' named entity 'KIT' named entity 'TIME' named entity 'RESULT' named entity 'PERFORMANCE' named entity 'SAMPLES' named entity 'CLINICAL' named entity 'MORBIDITY AND MORTALITY' named entity 'BACKGROUND' named entity 'TYPICAL' named entity 'CLINICAL EVALUATION' named entity '356' named entity 'ITS' named entity 'STUDY DESIGN' named entity 'COMPARED' named entity 'ROUTINE' named entity 'POPULATIONS' named entity 'TEST' named entity 'PATIENTS' named entity 'EVALUATION' named entity 'DETECTION' named entity 'MLPA METHOD' named entity 'PATIENTS' named entity 'SPECTRUM' named entity 'CONCORDANT' named entity 'RESPIRATORY' named entity 'RESPIRATORY PATHOGENS' named entity 'ASSAY' named entity 'POSITIVE' named entity 'TTR' named entity 'REDUCED' named entity 'RESPIRATORY TRACT INFECTIONS' named entity 'TO DETECT' named entity 'BACTERIAL' named entity 'VIRAL AND' named entity 'DUE TO' named entity 'COMPARISON' named entity 'PATHOGENS' named entity 'RESPIRATORY PATHOGENS' named entity 'HANDS' named entity 'OBJECTIVES' named entity 'SIGNIFICANT' named entity 'EXCELLENT' named entity 'CAUSE' named entity 'RESPIRATORY SAMPLES' named entity 'PREVIOUSLY' named entity 'EVALUATED' named entity 'COMPREHENSIVE'

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