About: Development of a TaqMan-based real-time RT-PCR assay for the detection of SADS-CoV associated with severe diarrhea disease in pigs

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About: Development of a TaqMan-based real-time RT-PCR assay for the detection of SADS-CoV associated with severe diarrhea disease in pigs Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Development of a TaqMan-based real-time RT-PCR assay for the detection of SADS-CoV associated with severe diarrhea disease in pigs
Creator	Zhou, Ling Li, Di Ma, Jing-Yun Mai, Kai-Jie Sun, Yuan Wu, Zi-Xian Chen, Gui-Hua Cheng, Jian Bai, Yang Wu, Jiao-Ling Wu, Rui-Ting Zhang, Xiang-Bin Zhou, Zhi-Hai
Source	Elsevier; Medline; PMC
abstract	Abstract Swine acute diarrhea syndrome coronavirus (SADS-CoV) is a novel coronavirus which was first reported in southern China in 2017. It can cause severe diarrhea disease in pigs. In order to detect this new emerging virus rapidly and reliably, a TaqMan-based real-time RT-PCR assay was established in this study. Specific primers and probe were designed and synthesized based on the conserved region within the N gene of the viral genome. Results showed that the lowest limit of detection was 3.0 × 101 copies/μL. This approach was specific for SADS-CoV, and there were no cross-reaction observed against other 15 swine viruses. It was 10 times more sensitive than the conventional PCR and gave higher SADS-CoV positive detection rate (70.69%, 123/174) than the conventional PCR (51.15%, 89/174) from clinical samples. These data indicated that the TaqMan-based real-time RT-PCR assay established here was an effective method with high sensitivity, specificity and reproducibility for faster and more accurate detection and quantification of SADS-CoV.
has issue date	2018-05-31(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2018.02.002
bibo:pmid	29427670
has license	els-covid
sha1sum (hex)	2ccc1adec85afa08657d6691bc0b8c973043a7cc
schema:url	https://doi.org/10.1016/j.jviromet.2018.02.002
resource representing a document's title	Development of a TaqMan-based real-time RT-PCR assay for the detection of SADS-CoV associated with severe diarrhea disease in pigs
has PubMed Central identifier	PMC7113665
has PubMed identifier	29427670
schema:publication	Journal of Virological Methods
resource representing a document's body	covid:2ccc1adec85afa08657d6691bc0b8c973043a7cc#body_text
is schema:about of	named entity 'TAQMAN' named entity 'specific' named entity 'copies' named entity 'China' named entity 'REAL-TIME RT-PCR' named entity 'FASTER' named entity 'DETECTION' named entity 'TIMES' named entity 'ACCURATE' named entity 'VIRUS' named entity 'RT-PCR ASSAY' named entity 'NEW' named entity 'ORDER' named entity 'SWINE ACUTE DIARRHEA SYNDROME CORONAVIRUS' named entity 'OBSERVED' named entity 'REPORTED' named entity 'PIGS' named entity 'THESE' named entity 'COPIES' named entity 'TO DETECT' named entity 'DISEASE' named entity 'METHOD' named entity 'IS A' named entity 'SENSITIVE' named entity 'LOWEST' named entity 'SAMPLES' named entity 'ESTABLISHED' named entity 'RATE' named entity '2851' named entity 'N GENE' named entity 'QUANTIFICATION' named entity 'CROSS-REACTION' named entity 'PROBE' named entity 'PRIMERS' named entity 'SOUTHERN' named entity 'BCL' named entity 'NOVEL CORONAVIRUS' named entity 'HERE' named entity 'GAVE' named entity 'HIGH SENSITIVITY' named entity 'SWINE' named entity 'POSITIVE' named entity 'PCR' named entity 'REAL-TIME RT-PCR' named entity 'REALTIME' named entity 'VIRAL GENOME' named entity 'SPECIFICITY' named entity 'HIGHER' named entity 'RT-PCR ASSAY' named entity 'DISEASE' named entity 'PIGS' named entity 'DETECTION' named entity 'DEVELOPMENT' named entity 'SADS-COV' named entity 'SPECIFIC' named entity 'INDICATED' named entity 'APPROACH' named entity 'CHINA' named entity 'SYNTHESIZED' named entity 'STUDY' named entity 'RESULTS' named entity 'EFFECTIVE' named entity 'CONVENTIONAL' named entity 'LIMIT OF DETECTION' named entity 'TAQMAN' named entity 'DATA' named entity 'CLINICAL' named entity 'CAUSE' named entity 'CONSERVED REGION'

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