About: Sequence analysis of open reading frames (ORFs) 2 to 4 of a U.S. isolate of porcine reproductive and respiratory syndrome virus

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About: Sequence analysis of open reading frames (ORFs) 2 to 4 of a U.S. isolate of porcine reproductive and respiratory syndrome virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Sequence analysis of open reading frames (ORFs) 2 to 4 of a U.S. isolate of porcine reproductive and respiratory syndrome virus
Creator	Meng, X.-J Morozov, I Paul, P
Source	PMC
abstract	The sequence of ORFs 2 to 4 of a U.S. isolate of porcine reproductive and respiratory syndrome virus (PRRSV), ATCC VR2385, was determined by analysis of a cDNA λ library. The cDNA clones containing PRRSV specific sequences were selected using a VR2385 ORF 4 specific PCR probe and sequenced. The ORFs 2, 3 and 4 overlapped each other and encoded polypeptides with predicted M(r) of 29.5 kDa (ORF 2), 28.7 kDa (ORF 3) and 19.5 kDa (ORF 4), respectively. No overlap was found between ORFs 4 and 5, and instead there was a 10 bp sequence which separated these two ORFs. The nucleic acid homology with corresponding ORFs of the European PRRSV isolate Lelystad virus (LV) was 65% for ORF 2, 64% for ORF 3 and 66% for ORF 4. Comparison of the ORF 4 sequences of VR2385 with that of another U.S. isolate MN-1b revealed only 86% amino acid sequence homology and the presence of deletions in the ORF 4 of MN-1b. Our results further strengthen the observation that there is sequence variation between US and European PRRSV isolates.
has issue date	1995-01-01(xsd:dateTime)
bibo:doi	10.1007/bf01322758
bibo:pmid	7646363
has license	no-cc
sha1sum (hex)	2cacfa1244712347061ae8f7e3ba28f05918010e
schema:url	https://doi.org/10.1007/bf01322758
resource representing a document's title	Sequence analysis of open reading frames (ORFs) 2 to 4 of a U.S. isolate of porcine reproductive and respiratory syndrome virus
has PubMed Central identifier	PMC7087238
has PubMed identifier	7646363
schema:publication	Arch Virol
resource representing a document's body	covid:2cacfa1244712347061ae8f7e3ba28f05918010e#body_text
is schema:about of	named entity 'REPORT' named entity 'PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS' named entity 'SEQUENCE ANALYSIS' named entity 'FOUND' named entity 'AMINO ACID SEQUENCE HOMOLOGY' named entity 'CDNA' named entity 'THESE' named entity 'VIRUS' named entity 'SEPARATED' named entity 'SEQUENCE VARIATION' named entity 'SEQUENCES' named entity 'ENCODED' named entity '19.5' named entity 'kDa' named entity 'ISOLATE' covid:arg/2cacfa1244712347061ae8f7e3ba28f05918010e named entity 'SELECTED' named entity 'M R' named entity 'U.S.' named entity 'PCR' named entity 'SEQUENCE' named entity 'KDA' named entity 'PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS' named entity 'PRESENCE OF' named entity 'OVERLAP' named entity 'ISOLATE' named entity 'OVERLAPPED' named entity 'COMPARISON' named entity 'U.S.' named entity 'PREDICTED' named entity 'EUROPEAN' named entity 'HOMOLOGY' named entity 'SPECIFIC' named entity 'ANALYSIS' named entity 'OUR' named entity 'OBSERVATION' named entity 'DETERMINED BY' named entity 'USING' named entity 'BRIEF' named entity 'OPEN READING FRAMES' named entity 'CONTAINING' named entity 'LIBRARY' named entity 'ORF' named entity 'POLYPEPTIDES' named entity 'NUCLEIC ACID' named entity 'REVEALED' named entity 'PROBE' named entity 'ATCC' named entity 'ORFS' named entity 'WAS A' named entity 'RESULTS' named entity 'CLONES' named entity 'kDa' named entity 'polypeptides' named entity 'selected' named entity 'virus' named entity 'isolates' named entity 'ORF' named entity 'kDa' named entity 'ORF' named entity 'PRRSV' named entity 'open reading frames' named entity 'Sequence analysis' named entity 'ORF' named entity 'amino acid' named entity 'homology' named entity 'ORFs' named entity 'hydrophobic' named entity 'ORF' named entity 'protein' named entity 'mRNAs' named entity 'non-coding region' named entity 'glycoprotein' named entity 'ORF' named entity 'glycoprotein' named entity 'ORFs' named entity 'virulence' named entity 'ORFs' named entity 'ORF'

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