About: SARS-CoV-2 Quasispecies Mediate Rapid Virus Evolution and Adaptation

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About: SARS-CoV-2 Quasispecies Mediate Rapid Virus Evolution and Adaptation Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	SARS-CoV-2 Quasispecies Mediate Rapid Virus Evolution and Adaptation
Creator	Ludwig, Stephan Brunotte, Linda Kröger, Andrea Eschke, Kathrin Cicin-Sain, Luka Markotić, Alemka Kim, Yeonsu Kurolt, Ivan-Christian Abassi, Leila Chaudhry, M Grashoff, Martina Klawonn, Frank
Source	BioRxiv
abstract	The pandemic spread of SARS-CoV-2 and the resulting global healthcare emergency warrants a better understanding of its biology. The potential of SARS-CoV-2 evolution to create novel dangerous variants remains underexplored. Thus, we passaged SARS-CoV-2 in defined conditions and determined its genomic adaptation dynamics. We demonstrate the presence of remarkably stable SARS-CoV-2 quasispecies. We further show that the quasispecies nature of the virus population ensured rapid adaptation of the spike PRRARS motif upon passaging in Vero cells. On the other hand, SARS-CoV-2 replication in TMPRSS2 expressing cells led to a reverse mutation at the same site. We observed the emergence of novel mutations in envelope protein upon virus culture in Calu-3 and Caco-2 cells. Finally, we show that the heparan sulfate-binding motif (PRRARS) of the SARS-CoV-2 S protein acted as a determinant of negative growth selection. Overall, our research has far-reaching implications for development of antiviral strategies, suggesting viral quasispecies may facilitate rapid emergence of escape mutants under selection pressure, such as the treatment with antivirals against SARS-CoV-2.
has issue date	2020-08-10(xsd:dateTime)
bibo:doi	10.1101/2020.08.10.241414
has license	biorxiv
sha1sum (hex)	2be09a95719c333aad9b5d2d934516ae8921901b
schema:url	https://doi.org/10.1101/2020.08.10.241414
resource representing a document's title	SARS-CoV-2 Quasispecies Mediate Rapid Virus Evolution and Adaptation
schema:publication	bioRxiv
resource representing a document's body	covid:2be09a95719c333aad9b5d2d934516ae8921901b#body_text
is schema:about of	named entity 'determined' named entity 'spike' named entity 'reverse' named entity 'SARS-COV-2' covid:arg/2be09a95719c333aad9b5d2d934516ae8921901b named entity 'acted' named entity 'evolution' named entity 'culture' named entity 'mutants' named entity 'development' named entity 'mutations' named entity 'motif' named entity 'SARS-CoV-2' named entity 'Virus' named entity 'Rapid' named entity 'envelope protein' named entity 'evolution' named entity 'Vero cells' named entity 'Caco-2' named entity 'heparan sulfate' named entity 'virus' named entity 'mutation rate' named entity 'carboxymethylcellulose' named entity 'SARS-CoV' named entity 'furin' named entity 'mutation' named entity 'endosomal' named entity 'virus' named entity 'furin' named entity 'Calu-3' named entity 'mutation' named entity 'ExoN' named entity 'subdominant' named entity 'L-glutamine' named entity 'virulence' named entity 'passaging' named entity 'molecular basis' named entity 'virus entry' named entity 'SARS-CoV-2' named entity 'cleavage site' named entity 'single point mutation' named entity 'motif' named entity 'endosomal' named entity 'Vero E6' named entity 'de Haan' named entity 'L-glutamine' named entity 'cleavage' named entity 'SARS CoV' named entity 'heparin' named entity 'hydrogen bonds' named entity 'Heparin' named entity 'arginine' named entity 'ACE2' named entity 'infection' named entity 'Tortorici' named entity 'mutation rate' named entity 'supernatant' named entity 'infection' named entity 'mutation rate' named entity 'cleavage site' named entity 'cleavage site' named entity 'GISAID' named entity 'de Haan' named entity 'deep sequencing' named entity 'binding motif' named entity 'virus' named entity 'hpi' named entity 'virus' named entity 'quasispecies' named entity 'cell surface' named entity 'mutation' named entity 'viruses' named entity 'TMPRSS2'

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