About: Simultaneous Detection and Differentiation of Human Papillomavirus Genotypes 6, 11, 16 and 18 by AllGlo Quadruplex Quantitative PCR

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About: Simultaneous Detection and Differentiation of Human Papillomavirus Genotypes 6, 11, 16 and 18 by AllGlo Quadruplex Quantitative PCR Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Simultaneous Detection and Differentiation of Human Papillomavirus Genotypes 6, 11, 16 and 18 by AllGlo Quadruplex Quantitative PCR
Creator	Tang, Yi-Wei Chen, Yu Li, Lanjuan Wang, Baohong Wu, Shenghai Yu, Daojun
Source	Medline; PMC
abstract	BACKGROUND: Human papillomaviruses (HPV) are classified into high-risk HPV and low-risk HPV. The most common high-risk HPV types in cervical cancer are HPV 16 and 18, and the most common low-risk types causing genital warts are HPV 6 and HPV 11. In this study, applying novel AllGlo fluorescent probes, we established a quadruplex quantitative PCR method to simultaneously detect and differentiate HPV 6, 11, 16 and 18 in a single tube. METHODS: The specificity, the sensitivity, the detection limit, the reproducibility and the standard curve of this method were examined. Finally, clinical samples that had been tested previously by TaqMan PCR and HPV GenoArray (GA) test were used to verify the accuracy and sensitivity of the method. RESULTS: The assay has a sensitivity of 10(1) to 10(2) copies/test and a linear detection range from 10(1) to 10(8) copies/test. The mean amplification efficiencies for HPV 6, 11, 16, and 18 were 0.97, 1.10, 0.93 and 1.20, respectively, and the mean correlation coefficient (r(2)) of each standard curve was above 0.99 for plasmid templates ranging from 10(3) to 10(7) copies/test. There was 100% agreement between the AllGlo quadruplex quantitative PCR, HPV GA test and TaqMan uniplex qPCR methods. CONCLUSIONS: AllGlo quadruplex quantitative PCR in a single tube has the advantages of relatively high throughput, good reproducibility, high sensitivity, high specificity, and a wide linear range of detection. The convenient single tube format makes this assay a powerful tool for the studies of mixed infections by multiple pathogens, viral typing and viral load quantification.
has issue date	2012-11-09(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0048972
bibo:pmid	23152833
has license	cc-by
sha1sum (hex)	2b10c237281a6e5d61e8fff995dc8920465eda56
schema:url	https://doi.org/10.1371/journal.pone.0048972
resource representing a document's title	Simultaneous Detection and Differentiation of Human Papillomavirus Genotypes 6, 11, 16 and 18 by AllGlo Quadruplex Quantitative PCR
has PubMed Central identifier	PMC3494670
has PubMed identifier	23152833
schema:publication	PLoS One
resource representing a document's body	covid:2b10c237281a6e5d61e8fff995dc8920465eda56#body_text
is schema:about of	named entity 'LOW-RISK HPV' named entity 'APPLYING' named entity 'HUMAN PAPILLOMAVIRUSES' named entity 'LOW' named entity 'TUBE' named entity 'HPV 11' named entity 'BACKGROUND' named entity 'CAUSING' named entity 'SINGLE' named entity 'HPV' named entity 'HPV' named entity 'DIFFERENTIATION' named entity 'CLASSIFIED' named entity 'HPV TYPES' named entity 'TYPES' named entity 'STUDY' named entity 'PROBES' named entity 'RISK' named entity 'FLUORESCENT' named entity 'NOVEL' named entity 'QUANTITATIVE PCR' named entity 'HUMAN PAPILLOMAVIRUS ' named entity 'QUANTITATIVE PCR' named entity 'DETECTION' named entity 'SIMULTANEOUS' named entity 'QUADRUPLEX' named entity 'GENOTYPES' named entity 'SIMULTANEOUSLY' named entity 'DETECT' named entity 'COMMON' named entity 'QUADRUPLEX' named entity 'DIFFERENTIATE' named entity 'HPV 16' named entity 'GENITAL WARTS' named entity 'CERVICAL CANCER' named entity 'ESTABLISHED' named entity 'HPV' named entity 'HIGH-RISK HPV' covid:arg/2b10c237281a6e5d61e8fff995dc8920465eda56 named entity 'simultaneously' named entity 'HPV' named entity 'Human papillomaviruses' named entity 'Genotypes' named entity 'Quantitative PCR' named entity 'HPV' named entity 'fluorescence' named entity 'qPCR' named entity 'control experiments' named entity 'qPCR' named entity 'plasmid' named entity 'Guangzhou' named entity 'fluorescence' named entity 'fluorescence' named entity 'fluorophores' named entity 'quantitative PCR' named entity 'single nucleotide polymorphism' named entity 'annealing temperature' named entity 'qPCR' named entity 'quantitative PCR' named entity 'fluorescence' named entity 'HPV' named entity 'HPV' named entity 'HPV' named entity 'PCR technique' named entity 'PCR' named entity 'E. coli' named entity 'quantitative PCR' named entity 'fluorescence' named entity 'drug development' named entity 'fluorescence' named entity 'HPV' named entity 'quantitative PCR' named entity 'genital warts' named entity 'clinical laboratory' named entity 'China' named entity 'HPV'

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