About: Recombination between satellite RNAs of turnip crinkle virus.

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About: Recombination between satellite RNAs of turnip crinkle virus. Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Recombination between satellite RNAs of turnip crinkle virus.
Creator	Carpenter2, Clifford Cascone1, Pamela Li2, Xiao Simon1, Anne
source	PMC
abstract	Turnip crinkle virus (TCV) is associated with satellite (sat) RNAs (sat-RNA D, sat-RNA F), defective interfering (DI) RNAs (DI RNA G, DI1 RNA), and one RNA with properties of both sat-RNAs and DI RNAs (sat-RNA C). When plants were inoculated with TCV, sat-RNA D and in vitro sat-RNA C transcripts containing non-viable mutations in the 5' domain, recombinant sat-RNAs were recovered. These recombinants were composed of sat-RNA D at the 5' end and sat-RNA C sequences at the 3' end. Analysis of 20 independent recombination junctions revealed that unequal crossing-over had occurred in planta in a region of sequence similarity between the two sat-RNAs which resulted in the duplication of 3-16 nucleotides. Thirty percent of the sat-RNA recombinants also had one to three additional nucleotides inserted at the crossover junctions which did not correspond to either sat-RNA C or sat-RNA D sequence. The right side of the recombination junctions always began with one of three consecutive nucleotides of sat-RNA C. Based on the similarity between this sequence of sat-RNA C, the right side junction of DI RNA G and the 5' end of TCV, as well as the sequence similarity between right side junctions of DI1 RNA and sat-RNA C and the 5' end of the sat-RNAs, a replicase-driven copy choice mechanism is proposed.(ABSTRACT TRUNCATED AT 250 WORDS)
has issue date	1990-06-01(xsd:dateTime)
bibo:pmid	1693330
has license	no-cc
sha1sum (hex)	2aed2399bb1ab45ff1b0ee68e70770aec3e3f129
resource representing a document's title	Recombination between satellite RNAs of turnip crinkle virus.
has PubMed Central identifier	PMC551873
has PubMed identifier	1693330
schema:publication	EMBO J
resource representing a document's body	covid:2aed2399bb1ab45ff1b0ee68e70770aec3e3f129#body_text
is schema:about of	named entity 'RNAs' named entity 'RNAs' named entity 'sequence' named entity 'mutations' named entity 'Analysis' named entity 'COMPOSED OF' named entity 'MECHANISM' named entity 'DID' named entity 'CROSSOVER' named entity 'PERCENT' named entity 'RIGHT SIDE OF' covid:arg/2aed2399bb1ab45ff1b0ee68e70770aec3e3f129 named entity 'internal' named entity 'sequence similarity' named entity 'TCV' named entity 'strand' named entity 'wild-type' named entity 'cDNA cloning' named entity 'ligated' named entity 'RNA' named entity 'RNA' named entity 'BRL' named entity 'RNA' named entity 'region' named entity 'recombination' named entity 'recombinants' named entity 'RNA' named entity 'RNA' named entity 'sequence similarity' named entity 'RNA' named entity '5' end' named entity '3' end' named entity 'recombinants' named entity 'RNA' named entity 'recombinant' named entity 'RNAs' named entity 'RNA' named entity 'RNA' named entity 'sequence similarity' named entity 'RNA' named entity 'TCV' named entity 'Recombination' named entity 'nucleotides' named entity 'RNA' named entity 'nucleotides' named entity 'RNA' named entity 'recombinant' named entity 'RNA virus' named entity 'RNA' named entity 'RNA' named entity 'RNA' named entity 'motif' named entity 'nucleotides' named entity 'TCV' named entity 'turnip crinkle virus' named entity 'multimers' named entity 'inocula' named entity 'RNA' named entity 'motif' named entity 'nucleotides' named entity 'recombinant' named entity 'avirulent' named entity 'nucleotides' named entity 'RNA' named entity 'nucleotides' named entity 'RNA' named entity 'TCV' named entity 'TCV' named entity 'sequence similarities' named entity 'DTT' named entity 'nucleotides' named entity 'RNA' named entity 'heterogeneity' named entity 'poliovirus' named entity 'TCV' named entity 'cDNA' named entity 'reverse transcriptase' named entity 'cDNA' named entity 'homologous sequences' named entity '3' end'

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