About: Genotyping of clinically relevant human adenoviruses by array-in-well hybridization assay

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About: Genotyping of clinically relevant human adenoviruses by array-in-well hybridization assay Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Genotyping of clinically relevant human adenoviruses by array-in-well hybridization assay
Creator	Waris, M Arppe, R Harju, E Hattara, L Hö Lsä, J Saviranta, P Soukka, T Ylihärsilä, M
Source	Elsevier; Medline; PMC
abstract	A robust oligonucleotide array-in-well hybridization assay using novel up-converting phosphor reporter technology was applied for genotyping clinically relevant human adenovirus types. A total of 231 adenovirus-positive respiratory, ocular swab, stool and other specimens from 219 patients collected between April 2010 and April 2011 were included in the study. After a real-time PCR amplification targeting the adenovirus hexon gene, the array-in-well assay identified the presence of B03 (n = 122; 57.5% of patients), E04 (29; 13.7%), C02 (21; 9.9%), D37 (14; 6.6%), C01 (12; 5.7%), C05 (5; 2.4%), D19 (4; 1.9%), C06 (2; 0.9%), D08 (1; 0.5%), A31 (1; 0.5%) and F41 (1; 0.5%) genotypes among the clinical sample panel. The typing result was obtained for all specimens that could be amplified (n = 223; 97%), and specificity of the typing was confirmed by sequencing specimens representing each of the different genotypes. No hybridization signal was obtained in adenovirus-negative specimens or specimens with other viruses (n = 30). The array-in-well hybridization assay has great potential as a rapid and multiplex platform for the typing of clinically relevant human adenovirus genotypes in different specimen types.
has issue date	2014-12-13(xsd:dateTime)
bibo:doi	10.1111/j.1469-0691.2012.03926.x
bibo:pmid	22712766
has license	no-cc
sha1sum (hex)	2ad01e32b5e2293e6c2e87f5bfa2b1cff45b6e7a
schema:url	https://doi.org/10.1111/j.1469-0691.2012.03926.x
resource representing a document's title	Genotyping of clinically relevant human adenoviruses by array-in-well hybridization assay
has PubMed Central identifier	PMC7129513
has PubMed identifier	22712766
schema:publication	Clin Microbiol Infect
resource representing a document's body	covid:2ad01e32b5e2293e6c2e87f5bfa2b1cff45b6e7a#body_text
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