About: Characterization of an Immunodominant Epitope in the Endodomain of the Coronavirus Membrane Protein

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About: Characterization of an Immunodominant Epitope in the Endodomain of the Coronavirus Membrane Protein Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Characterization of an Immunodominant Epitope in the Endodomain of the Coronavirus Membrane Protein
Creator	Feng, Li Chen, Jianfei Dong, Hui Shi, Da Shi, Hongyan Zhang, Xin Zhu, Yunnuan Dixon, Linda Graham, Simon
Source	Medline; PMC
abstract	The coronavirus membrane (M) protein acts as a dominant immunogen and is a major player in virus assembly. In this study, we prepared two monoclonal antibodies (mAbs; 1C3 and 4C7) directed against the transmissible gastroenteritis virus (TGEV) M protein. The 1C3 and 4C7 mAbs both reacted with the native TGEV M protein in western blotting and immunofluorescence (IFA) assays. Two linear epitopes, 243YSTEART249 (1C3) and 243YSTEARTDNLSEQEKLLHMV262 (4C7), were identified in the endodomain of the TGEV M protein. The 1C3 mAb can be used for the detection of the TGEV M protein in different assays. An IFA method for the detection of TGEV M protein was optimized using mAb 1C3. Furthermore, the ability of the epitope identified in this study to stimulate antibody production was also evaluated. An immunodominant epitope in the TGEV membrane protein endodomain was identified. The results of this study have implications for further research on TGEV replication.
has issue date	2016-12-10(xsd:dateTime)
bibo:doi	10.3390/v8120327
bibo:pmid	27973413
has license	cc-by
sha1sum (hex)	2ace5853970e68deefd9800fdd135a266237884a
schema:url	https://doi.org/10.3390/v8120327
resource representing a document's title	Characterization of an Immunodominant Epitope in the Endodomain of the Coronavirus Membrane Protein
has PubMed Central identifier	PMC5192388
has PubMed identifier	27973413
schema:publication	Viruses
resource representing a document's body	covid:2ace5853970e68deefd9800fdd135a266237884a#body_text
is schema:about of	named entity 'CHARACTERIZATION' named entity 'M protein' named entity 'immunofluorescence' named entity 'linear' named entity 'mAb' named entity 'membrane protein' named entity 'IMMUNOGEN' named entity 'METHOD' named entity 'PROTEIN ' named entity 'ABILITY' named entity 'WESTERN BLOTTING' named entity 'PREPARED' named entity 'HAVE' named entity 'EVALUATED' named entity 'LINEAR' named entity 'IFA' named entity 'ANTIBODY PRODUCTION' named entity 'DETECTION' named entity 'EPITOPE' named entity 'MEMBRANE' named entity 'CORONAVIRUS' named entity 'MONOCLONAL ANTIBODIES' named entity 'REPLICATION' named entity 'MEMBRANE PROTEIN' named entity 'MAB' named entity 'IMMUNOFLUORESCENCE' named entity 'NATIVE' named entity 'IS A' named entity 'ACTS' named entity 'A MAJOR' named entity 'IDENTIFIED' named entity '1C3' named entity 'MEMBRANE PROTEIN' named entity 'EPITOPES' named entity 'DIFFERENT' named entity 'STUDY' named entity 'DOMINANT' named entity 'STIMULATE' named entity 'TRANSMISSIBLE GASTROENTERITIS VIRUS' named entity 'RESEARCH' named entity 'RESULTS' named entity 'EPITOPE' named entity 'DIRECTED' named entity 'USING' named entity 'USED' named entity 'M PROTEIN' named entity 'VIRUS ASSEMBLY' named entity 'CORONAVIRUS' covid:arg/2ace5853970e68deefd9800fdd135a266237884a named entity 'M protein' named entity 'study' named entity 'reacted' named entity 'mAb' named entity 'evaluated' named entity 'M protein' named entity 'Characterization' named entity 'epitope' named entity 'protein' named entity 'western blotting' named entity 'epitope' named entity 'Immunodominant' named entity 'transmissible gastroenteritis virus' named entity 'coronavirus' named entity 'Coronavirus' named entity 'coronavirus' named entity 'immunofluorescence' named entity 'monoclonal antibodies' named entity 'TGEV' named entity 'mAbs' named entity 'virus' named entity 'epitopes' named entity 'immunogen' named entity 'Epitope'

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