About: A ‘minimal’ approach in design of flavivirus infectious DNA

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About: A ‘minimal’ approach in design of flavivirus infectious DNA Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A ‘minimal’ approach in design of flavivirus infectious DNA
Creator	Cominelli, Fabio Mishin, Vasiliy Yamshchikov, Vladimir
Source	Elsevier; Medline; PMC
abstract	Abstract The ‘infectious DNA’ approach, which is based on in vivo transcription of (+)RNA virus genome cDNA cassettes from eukaryotic promoters in transfected cells, became a popular alternative to the classical scheme in the infectious clone methodology. Its use, however, is often limited by the instability of plasmids due to a transcriptional activity of eukaryotic promoters in Escherichia coli resulting in synthesis of products toxic for the bacterial host. Using a highly unstable representative infectious clone of Japanese encephalitis (JE) flavivirus, we tested a new approach in design of such problematic ‘infectious DNA’ constructs, which is based on minimizing unwanted transcription in the bacterial host. A plasmid containing full genome size JE cDNA under control of the minimal cytomegalovirus (CMV) promoter can be propagated in E. coli with growth and stability characteristics similar to that of constructs controlled by the T7 promoter. Transfection of this plasmid into susceptible cells leads to the establishment of a productive infectious cycle. Reinsertion of the CMV enhancer at the 3′-end of the JE cassette substantially increased the specific infectivity without affecting the stability and growth characteristics of the construct. This approach can be useful when stabilization of infectious clones by modification of a viral cDNA cassette is not the feasible or suitable alternative.
has issue date	2001-12-04(xsd:dateTime)
bibo:doi	10.1016/s0168-1702(01)00371-9
bibo:pmid	11682130
has license	els-covid
sha1sum (hex)	2978685b4cf45c2cdc5e28559cacac625bbf9874
schema:url	https://doi.org/10.1016/s0168-1702%2801%2900371-9
resource representing a document's title	A ‘minimal’ approach in design of flavivirus infectious DNA
has PubMed Central identifier	PMC7127487
has PubMed identifier	11682130
schema:publication	Virus Research
resource representing a document's body	covid:2978685b4cf45c2cdc5e28559cacac625bbf9874#body_text
is schema:about of	named entity 'clone' named entity 'toxic' covid:arg/2978685b4cf45c2cdc5e28559cacac625bbf9874 named entity 'APPROACH' named entity 'CYTOMEGALOVIRUS' named entity 'PROMOTERS' named entity 'CDNA' named entity 'BASED' named entity 'DESIGN' named entity 'BACTERIAL' named entity 'ALTERNATIVE' named entity 'SCHEME' named entity 'METHODOLOGY' named entity 'TRANSCRIPTIONAL ACTIVITY' named entity 'LEADS' named entity 'CASSETTES' named entity 'CMV' named entity 'LIMITED BY' named entity 'CLASSICAL' named entity 'END' named entity 'TRANSFECTION' named entity 'GROWTH' named entity 'VIRAL' named entity 'GENOME' named entity 'E. COLI' named entity 'CONTROLLED BY' named entity 'ESCHERICHIA COLI' named entity 'STABILIZATION' named entity 'ITS' named entity 'INSTABILITY' named entity 'TRANSCRIPTION' named entity 'DUE TO' named entity 'TESTED' named entity 'SIMILAR' named entity 'CHARACTERISTICS' named entity 'EUKARYOTIC' named entity 'CLONES' named entity 'JAPANESE ENCEPHALITIS' named entity 'INFECTIOUS CLONE' named entity 'GROWTH CHARACTERISTICS' named entity 'DNA' named entity 'INFECTIVITY' named entity 'REINSERTION' named entity 'FLAVIVIRUS' named entity 'CELLS' named entity 'INFECTIOUS' named entity 'CYCLE' named entity 'IN VIVO' named entity 'DNA' named entity 'SUSCEPTIBLE' named entity 'APPROACH' named entity 'USING' named entity 'DESIGN' named entity 'SYNTHESIS' named entity 'INFECTIOUS' named entity 'STABILITY' named entity 'SUBSTANTIALLY INCREASED' named entity 'REPRESENTATIVE' named entity 'GENOME SIZE' named entity 'TOXIC' named entity 'UNDER CONTROL' named entity 'PLASMIDS' named entity 'USEFUL' named entity 'SUITABLE' named entity 'FEASIBLE' named entity 'SPECIFIC' named entity 'ESTABLISHMENT' named entity 'PRODUCTS' named entity 'PRODUCTIVE' named entity 'MODIFICATION' named entity 'PROMOTER' named entity 'PLASMID' named entity 'AFFECTING' named entity 'PROBLEMATIC' named entity 'USE' named entity 'HIGHLY' named entity 'ENHANCER' named entity 'FLAVIVIRUS' named entity 'CONTAINING'

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