About: Mismatch Extension During Strong Stop Strand Transfer and Minimal Homology Requirements for Replicative Template Switching During Moloney Murine Leukemia Virus Replication

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About: Mismatch Extension During Strong Stop Strand Transfer and Minimal Homology Requirements for Replicative Template Switching During Moloney Murine Leukemia Virus Replication Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Mismatch Extension During Strong Stop Strand Transfer and Minimal Homology Requirements for Replicative Template Switching During Moloney Murine Leukemia Virus Replication
Creator	Marr, Sharon Telesnitsky, Alice
Source	Elsevier; Medline; PMC
abstract	Abstract Reverse transcription requires two replicative template switches, called minus and plus strand strong stop transfer, and can include additional, recombinogenic switches. Donor and acceptor template homology facilitates both replicative and recombinogenic transfers, but homology-independent determinants may also contribute. Here, improved murine leukemia virus-based assays were established and the effects of varying extents of mismatches and complementarity between primer and acceptor template regions were assessed. Template switch accuracy was addressed by examining provirus structures, and efficiency was measured using a competitive titer assay. The results demonstrated that limited mismatch extension occurred readily during both minus and plus strand transfer. A strong bias for correct targeting to the U3/R junction and against use of alternate regions of homology was observed during minus strand transfer. Transfer to the U3/R junction was as accurate with five bases of complementarity as it was with an intact R, and as few as 3nt targeted transfer to a limited extent. In contrast, 12 base recombinogenic acceptors were utilized poorly and no accurate switch was observed when recombination acceptors retained only five bases of complementarity. These findings confirm that murine leukemia virus replicative and recombinogenic template switches differ in homology requirements, and support the notion that factors other than primer–template complementarity may contribute to strong stop acceptor template recognition.
has issue date	2003-07-18(xsd:dateTime)
bibo:doi	10.1016/s0022-2836(03)00597-7
bibo:pmid	12850138
has license	els-covid
sha1sum (hex)	2918a43791587306be37d4e23f804c1efdbdd8fa
schema:url	https://doi.org/10.1016/s0022-2836%2803%2900597-7
resource representing a document's title	Mismatch Extension During Strong Stop Strand Transfer and Minimal Homology Requirements for Replicative Template Switching During Moloney Murine Leukemia Virus Replication
has PubMed Central identifier	PMC7173232
has PubMed identifier	12850138
schema:publication	Journal of Molecular Biology
resource representing a document's body	covid:2918a43791587306be37d4e23f804c1efdbdd8fa#body_text
is schema:about of	named entity 'extent' named entity 'contrast' named entity 'murine' named entity 'targeting' named entity 'notion' named entity 'bases' named entity 'Template' named entity 'REGIONS' named entity 'BIAS' named entity 'PLUS STRAND' named entity 'EXAMINING' named entity 'extension' named entity 'measured' named entity 'called' named entity 'provirus' named entity 'assays' named entity 'transfection' named entity 'viral RNA' named entity 'RSV' named entity 'cross talk' named entity 'TM3' named entity 'minus strand' named entity 'Plasmids' named entity 'plasmids' named entity 'viral particles' named entity 'murine leukemia virus' named entity 'leukemia' named entity 'bases' named entity 'additional' named entity 'complementarity' named entity 'confirm' named entity 'minus strand' named entity 'recombinogenic' named entity 'recombinogenic' named entity 'strand' named entity 'proviral' named entity 'wild-type' named entity 'reverse transcription' named entity 'subcloned' named entity 'recombination' named entity 'intron' named entity 'polybrene' named entity 'PCR' named entity 'RNAs' named entity 'pUC' named entity 'titer' named entity 'cell line' named entity 'digestion' named entity 'Puromycin' named entity 'minus strand' named entity 'recombinogenic' named entity 'LTR' named entity 'genome' named entity 'LTRs' named entity 'viral RNA' named entity 'minus strand' named entity 'vector' named entity 'recombinogenic' named entity 'HIV-1' named entity 'LTR' named entity 'LTR' named entity 'transfection' named entity 'T4 polynucleotide kinase' named entity 'transfection' named entity 'RNAs' named entity 'RNA' named entity 'Figure 8' named entity 'vector' named entity 'homology' named entity 'viral genomes' named entity 'genome' named entity 'PCR' named entity 'plasmid' named entity 'vector' named entity 'ectopic site' named entity 'LTR' named entity 'MLV' named entity 'minus strand' named entity 'genetic elements' named entity 'restriction digestion'

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