About: Quantitative Fluorescence Quenching on Antibody-conjugated Graphene Oxide as a Platform for Protein Sensing

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About: Quantitative Fluorescence Quenching on Antibody-conjugated Graphene Oxide as a Platform for Protein Sensing Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Quantitative Fluorescence Quenching on Antibody-conjugated Graphene Oxide as a Platform for Protein Sensing
Creator	Li, Weiwei Huang, Ao Yao, Tianming Shi, Shuo
Source	PMC
abstract	We created an immunosensing platform for the detection of proteins in a buffer solution. Our sensing platform relies on graphene oxide (GO) nanosheets conjugated with antibodies to provide quantitative binding sites for analyte proteins. When analyte proteins and standard fluorescein-labelled proteins are competing for the binding sites, the assay exhibits quantitative fluorescence quenching by GO for the fluorescein-labelled proteins as determined by the analyte protein concentration. Because of this mechanism, measured fluorescence intensity from unquenched fluorescein-labelled protein was shown to increase with an increasing analyte protein concentration. As an alternative to the conventional enzyme-linked immunosorbent assay (ELISA), our method does not require an enzyme-linked second antibody for protein recognition and the enzyme for optical signal measurement. Thus, it is beneficial with its low cost and fewer systematic errors caused by the series of antigen-antibody recognition steps in ELISA. Immune globulin G (IgG) was introduced as a model protein to test our method and our results showed that the limit of detection for IgG was 4.67 pmol mL(−1) in the buffer solution. This sensing mechanism could be developed into a promising biosensor for the detection of proteins, which would broaden the spectrum of GO applications in both analytical biochemistry and clinical diagnosis.
has issue date	2017-01-13(xsd:dateTime)
bibo:doi	10.1038/srep40772
bibo:pmid	28084438
has license	cc-by
sha1sum (hex)	2822c9550311df8e6325df17b17cfde48c9ab6ab
schema:url	https://doi.org/10.1038/srep40772
resource representing a document's title	Quantitative Fluorescence Quenching on Antibody-conjugated Graphene Oxide as a Platform for Protein Sensing
has PubMed Central identifier	PMC5233999
has PubMed identifier	28084438
schema:publication	Sci Rep
resource representing a document's body	covid:2822c9550311df8e6325df17b17cfde48c9ab6ab#body_text
is schema:about of	named entity 'QUANTITATIVE' named entity 'IgG' named entity 'measured' named entity 'quantitative' named entity 'provide' named entity 'Graphene' named entity 'SENSING' named entity 'SERIES' named entity 'MEASUREMENT' named entity 'STEPS' named entity 'INTRODUCED' named entity 'LOW' named entity 'FLUORESCENCE INTENSITY' named entity 'TO TEST' named entity 'QUENCHING' named entity 'PROTEINS' named entity 'BENEFICIAL' named entity 'PROTEIN CONCENTRATION' named entity 'RECOGNITION' named entity 'ELISA' named entity 'COULD BE' named entity 'QUANTITATIVE' named entity 'INCREASING' named entity 'ITS' named entity 'BUFFER SOLUTION' named entity 'MECHANISM' named entity 'DETECTION OF PROTEINS' named entity 'SECOND' named entity 'ASSAY' named entity 'ANTIBODY' named entity 'TO PROVIDE' named entity 'MODEL' named entity '921' named entity 'GLOBULIN G' named entity 'CREATED' named entity 'BIOSENSOR' named entity 'FLUORESCEIN' named entity 'ANALYTE' named entity 'SPECTRUM' named entity 'SENSING' named entity 'PROTEIN ' named entity 'DETERMINED BY' named entity 'IGG' named entity 'RESULTS' named entity 'GRAPHENE OXIDE' named entity 'CONJUGATED' named entity 'STANDARD' named entity 'ALTERNATIVE' named entity 'PLATFORM' named entity 'APPLICATIONS' named entity 'CONVENTIONAL' named entity 'ANALYTICAL BIOCHEMISTRY' named entity 'BINDING SITES' named entity 'METHOD' named entity 'MEASURED' named entity 'COST' named entity 'ANTIBODIES' named entity 'ANTIBODY' named entity 'OPEN' named entity 'PROTEIN ' named entity 'GRAPHENE OXIDE' named entity 'FLUORESCENCE' named entity 'DOES NOT' named entity 'CAUSED BY' named entity 'IMMUNE GLOBULIN' named entity 'CLINICAL DIAGNOSIS' named entity 'ENZYME' named entity 'ANTIGEN' named entity 'PROTEIN RECOGNITION' named entity 'LIMIT OF DETECTION' named entity 'PMOL' named entity 'PLATFORM' named entity 'OPTICAL' named entity 'OUR' named entity 'EXHIBITS' named entity 'QUENCHING' named entity 'CONJUGATED' named entity 'ERRORS'

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