About: s-RT-MELT for rapid mutation scanning using enzymatic selection and real time DNA-melting: new potential for multiplex genetic analysis

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About: s-RT-MELT for rapid mutation scanning using enzymatic selection and real time DNA-melting: new potential for multiplex genetic analysis Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	s-RT-MELT for rapid mutation scanning using enzymatic selection and real time DNA-melting: new potential for multiplex genetic analysis
Creator	Li, Jin Wang, Lilin Berbeco, Ross Distel, Robert Jä Nne, Pasi Makrigiorgos, G
topic	covid:281b4272005dbc998e36b034b7fb528c9ba038cd#this
Source	PMC
abstract	The rapidly growing understanding of human genetic pathways, including those that mediate cancer biology and drug response, leads to an increasing need for extensive and reliable mutation screening on a population or on a single patient basis. Here we describe s-RT-MELT, a novel technology that enables highly expanded enzymatic mutation scanning in human samples for germline or low-level somatic mutations, or for SNP discovery. GC-clamp-containing PCR products from interrogated and wild-type samples are hybridized to generate mismatches at the positions of mutations over one or multiple sequences in-parallel. Mismatches are converted to double-strand breaks using a DNA endonuclease (Surveyor™) and oligonucleotide tails are enzymatically attached at the position of mutations. A novel application of PCR enables selective amplification of mutation-containing DNA fragments. Subsequently, melting curve analysis, on conventional or nano-technology real-time PCR platforms, detects the samples that contain mutations in a high-throughput and closed-tube manner. We apply s-RT-MELT in the screening of p53 and EGFR mutations in cell lines and clinical samples and demonstrate its advantages for rapid, multiplexed mutation scanning in cancer and for genetic variation screening in biology and medicine.
has issue date	2007-06-01(xsd:dateTime)
bibo:doi	10.1093/nar/gkm403
bibo:pmid	17545195
has license	cc-by-nc
sha1sum (hex)	281b4272005dbc998e36b034b7fb528c9ba038cd
schema:url	https://doi.org/10.1093/nar/gkm403
resource representing a document's title	s-RT-MELT for rapid mutation scanning using enzymatic selection and real time DNA-melting: new potential for multiplex genetic analysis
has PubMed Central identifier	PMC1919510
has PubMed identifier	17545195
schema:publication	Nucleic Acids Res
resource representing a document's body	covid:281b4272005dbc998e36b034b7fb528c9ba038cd#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/281b4272005dbc998e36b034b7fb528c9ba038cd
is schema:about of	named entity 'SELECTIVE' named entity 'APPLY' named entity 'EGFR' named entity 'CLOSED' named entity 'DNA' named entity 'REAL-TIME PCR ' named entity 'DRUG RESPONSE' named entity 'LEVEL' named entity 'EXPANDED ' named entity 'INCREASING' named entity 'NOVEL' named entity 'SAMPLES' named entity 'POSITION' named entity 'CANCER' named entity 'POPULATION' named entity 'HIGH' named entity 'GENETIC VARIATION' named entity 'NEED FOR' named entity 'BASIS' named entity 'POSITIONS' named entity 'AMPLIFICATION' named entity 'HERE' named entity 'extensive' named entity 'basis' named entity 'leads' named entity 'hybridized' named entity 'wild-type' named entity 'cancer' named entity 'genetic analysis' named entity 'USING' named entity 'NEW' named entity 'A DNA' named entity 'MUTATION' named entity 'CLINICAL' named entity 'PATHWAYS' named entity 'RAPIDLY' named entity 'TECHNOLOGY' named entity 'MELT' named entity 'MUTATION' named entity 'MULTIPLEX' named entity 'RAPID' named entity 'HUMAN' named entity 'STRAND BREAKS' named entity 'APPLICATION' named entity 'LEADS' named entity 'MEDIATE' named entity 'ENZYMATIC' named entity 'SCANNING' named entity 'GENETIC ANALYSIS' named entity 'REAL TIME' named entity 'MELTING' named entity 'USING' named entity 'HUMAN GENETIC' named entity 'PARALLEL' named entity 'GERMLINE' named entity 'UNDERSTANDING' named entity 'HIGHLY' named entity 'NANO-' named entity 'TAILS' named entity 'MULTIPLE SEQUENCES' named entity 'INCLUDING' named entity 'PATIENT' named entity 'ENZYMATIC' named entity 'ITS' named entity 'FRAGMENTS' named entity 'MELTING CURVE ANALYSIS' named entity 'OLIGONUCLEOTIDE' named entity 'THROUGHPUT' named entity 'SOMATIC MUTATIONS' named entity 'MEDICINE' named entity 'MELT' named entity 'POTENTIAL' named entity 'DNA' named entity 'RAPID'

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