About: Proteomic analysis of Pteropus alecto kidney cells in response to the viral mimic, Poly I:C

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Proteomic analysis of Pteropus alecto kidney cells in response to the viral mimic, Poly I:C
Creator	Bacic, Antony Ford, Kris Mok, Lawrence Shiell, Brian Michalski, Wojtek Wynne, James
topic	covid:27373ff091fe4a81ee4d6bfc09ece3995080c2ea#this
source	Medline; PMC
abstract	BACKGROUND: Bats are recognised as an important reservoir for a number of highly pathogenic zoonotic viruses. While many of these viruses cause severe and often fatal disease in humans, bats are able to coexist with these viruses without clinical signs of disease. The mechanism conferring this antiviral response is not fully understood. Here, we investigated the differential protein expression of immortalised Pteropus alecto kidney cells (PaKiT03) following transfection with the viral mimic, Poly I:C. Two complementary proteomic approaches, difference gel electrophoresis (DIGE) and isobaric tagging for relative and absolute quantitation (iTRAQ) were used to quantify changes in protein expression following Poly I:C stimulation at 4, 8 and 20 hr post treatment (hpt). RESULTS: The expression of ISG54 gene, a known responder to virus infection and Poly I:C treatment, was significantly induced in transfected cells compared with mock-transfected cells. Through iTRAQ analysis we show that Poly I:C up-regulates key glycolytic enzymes at 4 hpt within PaKiT03 cells. In contrast, at 20 hpt PaKiT03 cells down-regulated ribosomal subunit proteins. The analysis with DIGE of Poly I:C transfected PaKiT03 cells showed over 215 individual spots differentially regulated, however only 25 spots could be unambiguously identified by LC-MS/MS. Immunoblotting confirmed the up-regulation of Eno1 and Tpi1 in PaKiT03 cells following Poly I:C transfection. A comparison with human cells (HEK293T and HeLa) and one additional bat cell line (PaLuT02), demonstrated that glycolytic pathways are also induced in these cell types, but at different intensities. CONCLUSION: The two techniques, DIGE and iTRAQ identified largely overlapping sets of differentially expressed proteins, however DIGE unambiguously identified significantly less proteins than iTRAQ. Poly I:C induced a rapid metabolic shift towards glycolysis within the PaKiT03 cells at 4 hpt, presumably as a consequence of increased energy requirements. On the other hand ribosomal subunit proteins were seen as down-regulated by iTRAQ, these proteins may be the limiting factors in the translational machinery available for virus replication. This study provides new insight into the antiviral response of bat cells, highlighting the importance of energy metabolism. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12953-015-0081-6) contains supplementary material, which is available to authorized users.
has issue date	2015-11-02(xsd:dateTime)
bibo:doi	10.1186/s12953-015-0081-6
bibo:pmid	26535029
has license	cc-by
sha1sum (hex)	27373ff091fe4a81ee4d6bfc09ece3995080c2ea
schema:url	https://doi.org/10.1186/s12953-015-0081-6
resource representing a document's title	Proteomic analysis of Pteropus alecto kidney cells in response to the viral mimic, Poly I:C
has PubMed Central identifier	PMC4630911
has PubMed identifier	26535029
schema:publication	Proteome Sci
resource representing a document's body	covid:27373ff091fe4a81ee4d6bfc09ece3995080c2ea#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/27373ff091fe4a81ee4d6bfc09ece3995080c2ea
is schema:about of	named entity 'iTRAQ' named entity 'quantify' named entity 'highly' named entity 'zoonotic' named entity 'cells' named entity 'Pteropus' named entity 'CAUSE' named entity 'BATS' named entity 'BACKGROUND' named entity 'TRANSFECTION' named entity 'ABSOLUTE QUANTITATION' named entity 'QUANTIFY' named entity 'IMPORTANT' named entity 'PROTEOMIC' named entity 'HUMANS' named entity 'MIMIC' named entity 'STIMULATION' named entity 'CELLS' named entity 'UNDERSTOOD' named entity 'VIRUSES' named entity 'DIFFERENCE GEL ELECTROPHORESIS' named entity 'PTEROPUS ALECTO' named entity 'PROTEIN EXPRESSION' named entity 'INVESTIGATED' named entity 'RESPONSE TO' named entity 'POLY I' named entity 'PATHOGENIC' named entity 'FULLY' named entity 'ANTIVIRAL RESPONSE' named entity 'APPROACHES' named entity 'MIMIC' named entity 'CELLS' named entity 'KIDNEY' named entity 'HERE' named entity 'VIRAL' named entity 'PTEROPUS ALECTO' named entity 'RESERVOIR' named entity 'USED' named entity 'NUMBER OF' named entity 'THESE' named entity 'KIDNEY' named entity 'FATAL DISEASE' named entity 'POLY I' named entity 'POST TREATMENT' named entity 'TAGGING' named entity 'HIGHLY' named entity 'DIFFERENTIAL' named entity 'RELATIVE' named entity 'VIRAL' named entity 'PROTEOMIC ANALYSIS' named entity 'ZOONOTIC' named entity 'CHANGES' named entity 'DISEASE' named entity 'SEVERE' named entity 'FOLLOWING' named entity 'MECHANISM' covid:arg/27373ff091fe4a81ee4d6bfc09ece3995080c2ea named entity 'transfection' named entity 'disease' named entity 'alecto' named entity 'viruses' named entity 'protein' named entity 'Proteomic' named entity 'Poly I:C' named entity 'fatal disease' named entity 'zoonotic' named entity 'Proteomic analysis' named entity 'expression' named entity 'transfection' named entity 'human cell lines' named entity 'liquid chromatography-mass spectrometry' named entity 'Pgk1' named entity 'IgG' named entity 'down-regulated'

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