About: The design and application of DNA chips for early detection of SARS-CoV from clinical samples

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	The design and application of DNA chips for early detection of SARS-CoV from clinical samples
Creator	Zhang, Yan An, Shuang Cheng, Jing Cheng, Yun Guo, Lan Guo, Yong Hung, Tao Jiang, Di Li, Ze Li, Zhe Liu, Ou Rong, Li Ruan, Li Wang, Can Wang, Dong Zhang, Liang Zhang, Qiong Zheng, Zhong Wang, Jian-Wei Tao, Sheng-Ce Ren, Li-Li Du, Jian-Yu Fan, Xiao-Dong Gao, Hua-Fang Liu, Yan-Hua Lü, Zhan-Xiu Shan, Quan-Zhong Wang, Yan-Bin Yang, Hua-Wei Yang, Ren-Quan Zhang, Zhi-Wei Zhao, Chuan-Zan Zhao, Yan-Jun Zhou, Yi-Ming Zhu, Pei-Xuan Zuo, Huan-Cong
source	Elsevier; Medline; PMC
abstract	BACKGROUND: SARS coronavirus has been identified as the cause of severe acute respiratory syndrome (SARS). Few tests allow confirmation or exclusion of SARS within the first few days of infection. A gene chip is a useful tool for the study of microbial infections mainly for its capability of performing multi-target analysis in a single test. OBJECTIVES: Investigate the possibility of early detection of SARS virus from clinical samples using the gene chip-based method. STUDY DESIGN: We purified RNA from SARS-CoV obtained from routinely collected peripheral blood and sputum samples of 34 patients who had been identified as probable SARS patients by following the interim U.S. case definition. Four segments of the SARS-CoV were amplified using reverse transcription-nested PCR and the products examined using the 70-mer gene chips for SARS-CoV detection. RESULTS: A blind-test of both peripheral blood and sputum specimens lead to the positive detection of SARS-CoV in 31 out of 34 patients. SARS-CoV was not found in peripheral blood or sputum specimens from three patients. Two of the 34 patients were only 3 days post-onset of symptoms and were subsequently confirmed to be SARS positive. Our results indicate that the gene chip-based molecular test is specific for SARS-CoV and allows early detection of patients with SARS with detection rate about 8% higher than the single PCR test when the sputum sample is available.
has issue date	2005-01-19(xsd:dateTime)
bibo:doi	10.1016/j.jcv.2004.10.019
bibo:pmid	15911427
has license	no-cc
sha1sum (hex)	25cc93bafacf163c6e315809b41ef6d814c15b15
schema:url	https://doi.org/10.1016/j.jcv.2004.10.019
resource representing a document's title	The design and application of DNA chips for early detection of SARS-CoV from clinical samples
has PubMed Central identifier	PMC7108259
has PubMed identifier	15911427
schema:publication	J Clin Virol
resource representing a document's body	covid:25cc93bafacf163c6e315809b41ef6d814c15b15#body_text
is schema:about of	named entity 'MULTI' named entity 'COLLECTED' named entity 'EXCLUSION OF' named entity 'BLIND' named entity 'A GENE' named entity 'NESTED PCR ' named entity 'USEFUL' named entity 'TEST' named entity 'ABOUT' named entity 'AMPLIFIED' named entity 'PROBABLE' named entity 'PRODUCTS' named entity 'METHOD' named entity 'GENE CHIP' named entity 'POST' named entity 'USING' named entity 'OBJECTIVES' named entity 'INFECTION' named entity 'test' named entity 'gene' named entity 'gene' named entity 'reverse' named entity 'gene' named entity 'SARS-CoV' named entity 'SPUTUM SAMPLE' named entity 'POSSIBILITY OF' named entity 'CASE DEFINITION' named entity 'CONFIRMATION' named entity 'INTERIM' named entity 'POSITIVE' named entity 'OUR' named entity 'SINGLE' named entity 'RESULTS' named entity 'APPLICATION' named entity 'CLINICAL' named entity 'TARGET' named entity 'U.S.' named entity 'ANALYSIS' named entity 'PATIENTS' named entity 'BASED' named entity 'RATE' named entity 'MER' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME' named entity 'SPECIMENS' named entity 'BACKGROUND' named entity 'IS A'

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