About: SYBR Green real-time reverse transcription-polymerase chain reaction assay for the generic detection of coronaviruses

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: SYBR Green real-time reverse transcription-polymerase chain reaction assay for the generic detection of coronaviruses Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	SYBR Green real-time reverse transcription-polymerase chain reaction assay for the generic detection of coronaviruses
Creator	Belák, S Berg, M Blomberg, J Escutenaire, S Isaksson, M Klingeborn, B Mohamed, N Thorén, P
Source	Medline; PMC
abstract	Coronaviruses are etiologic agents of respiratory and enteric diseases in humans and in animals. In this study, a one-step real-time reverse transcription-polymerase chain reaction (RT-PCR) assay based on SYBR Green chemistry and degenerate primers was developed for the generic detection of coronaviruses. The primers, designed in the open reading frame 1b, enabled the detection of 32 animal coronaviruses including strains of canine coronavirus, feline coronavirus, transmissible gastroenteritis virus (TGEV), bovine coronavirus (BCoV), murine hepatitis virus (MHV) and infectious bronchitis virus (IBV). A specific amplification was also observed with the human coronaviruses (HCoV) HCoV-NL63, HCoV-OC43, HCoV-229E and severe acute respiratory syndrome coronavirus (SARS-CoV). The real-time RT-PCR detected down to 10 cRNA copies from TGEV, BCoV, SARS-CoV and IBV. In addition, the assay exhibited a high sensitivity and specificity on clinical samples from different animal species. The developed assay represents a potential tool for laboratory diagnostics and for detecting still uncharacterized coronaviruses.
has issue date	2006-08-28(xsd:dateTime)
bibo:doi	10.1007/s00705-006-0840-x
bibo:pmid	16941059
has license	no-cc
sha1sum (hex)	241e769da8cbe2dcd7c53c3a6656a04cb8fe678d
schema:url	https://doi.org/10.1007/s00705-006-0840-x
resource representing a document's title	SYBR Green real-time reverse transcription-polymerase chain reaction assay for the generic detection of coronaviruses
has PubMed Central identifier	PMC7087200
has PubMed identifier	16941059
schema:publication	Arch Virol
resource representing a document's body	covid:241e769da8cbe2dcd7c53c3a6656a04cb8fe678d#body_text
is schema:about of	named entity 'agents' named entity 'enabled' named entity 'detection' named entity 'detection' named entity 'reverse' named entity 'assay' named entity 'RESPIRATORY' named entity 'HCOV-229E' named entity 'HCOV-NL63' named entity 'PRIMERS' named entity 'OPEN READING FRAME' named entity 'DIFFERENT' named entity 'STEP' named entity 'REAL-TIME RT-PCR' named entity 'POTENTIAL' named entity 'STRAINS' named entity 'HUMANS' named entity 'TRANSMISSIBLE GASTROENTERITIS VIRUS' named entity 'DETECTION' named entity 'DEGENERATE' named entity 'MURINE HEPATITIS VIRUS' covid:arg/241e769da8cbe2dcd7c53c3a6656a04cb8fe678d named entity 'SYBR GREEN' named entity 'POLYMERASE CHAIN REACTION ASSAY' named entity 'REAL-TIME' named entity 'ENABLED' named entity 'LABORATORY' named entity 'CHAIN REACTION' named entity 'DETECTING' named entity 'GENERIC' named entity 'DETECTION' named entity 'CORONAVIRUSES' named entity 'GREEN ' named entity 'DETECTED' named entity 'FELINE CORONAVIRUS' named entity 'COPIES' named entity 'ANIMALS' named entity 'ANIMAL' named entity 'HCOV-OC43' named entity 'DIAGNOSTICS' named entity 'SARS-COV' named entity 'SAMPLES' named entity 'BOVINE CORONAVIRUS' named entity 'CANINE CORONAVIRUS' named entity 'GENERIC' named entity 'REVERSE TRANSCRIPTION' named entity 'STUDY' named entity 'HIGH SENSITIVITY' named entity 'HUMAN' named entity 'SPECIFIC' named entity 'AMPLIFICATION' named entity 'INCLUDING' named entity 'ASSAY' named entity 'CRNA' named entity 'SENSITIVITY AND SPECIFICITY' named entity 'CORONAVIRUSES' named entity 'ENTERIC' named entity 'PCR' named entity 'TOOL' named entity 'SYBR GREEN' named entity 'REVERSE' named entity 'ADDITION' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME CORONAVIRUS' named entity 'REAL-TIME' named entity 'OBSERVED' named entity 'DISEASES' named entity 'CLINICAL' named entity 'ANIMAL SPECIES' named entity 'INFECTIOUS BRONCHITIS VIRUS' named entity 'BASED' named entity 'GREEN CHEMISTRY' named entity 'diseases' named entity 'potential' named entity 'detecting'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software