About: Symposium review: Intravital imaging of the lactating mammary gland in live mice reveals novel aspects of milk-lipid secretion

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About: Symposium review: Intravital imaging of the lactating mammary gland in live mice reveals novel aspects of milk-lipid secretion Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Symposium review: Intravital imaging of the lactating mammary gland in live mice reveals novel aspects of milk-lipid secretion
Creator	Al, Mather Et, Al ; Mather Et Al ; Mather Et
Source	Elsevier; Medline; PMC
abstract	ABSTRACT Milk fat comprises membrane-coated droplets of neutral lipid, which constitute the predominant source of lipids for survival of the suckling neonate. From the perspective of the dairy industry, they are the basis for the manufacture of butter and essential ingredients in the production of cheese, yogurt, and specialty dairy produce. To provide mechanistic insight into the assembly and secretion of lipid droplets during lactation, we developed novel intravital imaging techniques using transgenic mice, which express fluorescently tagged marker proteins. The number 4 mammary glands were surgically prepared under a deep plane of anesthesia and the exposed glands positioned as a skin flap with intact vascular supply on the stage of a laser-scanning confocal microscope. Lipid droplets were stained by prior exposure of the glands to hydrophobic fluorescent BODIPY (boron-dipyrromethene) dyes and their formation and secretion monitored by time-lapse subcellular microscopy over periods of 1 to 2 h. Droplets were transported to the cell apex by directed (superdiffusive) motion at relatively slow and intermittent rates (0–2 µm/min). Regardless of size, droplets grew by numerous fusion events during transport and as they were budding from the cell enveloped by apical membranes. Surprisingly, droplet secretion was not constitutive but required an injection of oxytocin to induce contraction of the myoepithelium with subsequent release of droplets into luminal spaces. These novel results are discussed in the context of the current paradigm for milk fat synthesis and secretion and as a template for future innovations in the dairy industry.
has issue date	2019-03-31(xsd:dateTime)
bibo:doi	10.3168/jds.2018-15459
bibo:pmid	30471915
has license	els-covid
sha1sum (hex)	2406311fdb93aeb0257bff62ed5800ca5e2bd8ad
schema:url	https://doi.org/10.3168/jds.2018-15459
resource representing a document's title	Symposium review: Intravital imaging of the lactating mammary gland in live mice reveals novel aspects of milk-lipid secretion
has PubMed Central identifier	PMC7094374
has PubMed identifier	30471915
schema:publication	Journal of Dairy Science
resource representing a document's body	covid:2406311fdb93aeb0257bff62ed5800ca5e2bd8ad#body_text
is schema:about of	named entity 'FIGURE' named entity 'TO PROVIDE' named entity 'MEMBRANES' named entity 'FLUORESCENT' named entity 'LIPIDS' named entity 'OXYTOCIN' named entity 'MARKER' named entity 'PRODUCE' named entity 'DAIRY' named entity 'LIPID DROPLETS' named entity 'MICROSCOPY' named entity 'NEUTRAL' named entity 'MYOEPITHELIUM' named entity 'CONTEXT' named entity 'PERSPECTIVE' named entity 'DYES' named entity 'SYNTHESIS' named entity 'CHEESE' named entity 'RESULTS' named entity 'SLOW' named entity 'STEPS' named entity 'PRODUCTION' named entity 'BASIS' named entity 'SURVIVAL' named entity 'PARADIGM' named entity 'FUTURE' named entity 'BODIPY' named entity 'neonate' named entity 'stained' named entity 'lactation' named entity 'butter' named entity 'imaging' named entity 'basal' named entity 'transported' named entity 'number' named entity 'suckling' named entity 'plasma membrane' named entity 'paradigm' named entity 'secretion' named entity 'luminal' named entity 'LIPID DROPLETS' named entity 'SOURCE' named entity 'EXPOSED' named entity 'MOTION' named entity 'USING' named entity 'DROPLET' named entity 'THE CELL' named entity 'BUDDING' named entity 'DEEP' named entity 'TRANSGENIC MICE' named entity 'INTERMITTENT' named entity 'MAMMARY GLANDS' named entity 'PROTEINS' named entity 'ASSEMBLY' named entity 'VASCULAR SUPPLY' named entity 'ESSENTIAL' named entity 'RATES' named entity 'SECRETION' named entity 'INJECTION' named entity 'DURING TRANSPORT' named entity 'EXPRESS' named entity 'STAGE' named entity 'BPM' named entity 'LUMINAL' named entity 'CURRENT' named entity 'APM' named entity 'APICAL PLASMA MEMBRANE' named entity 'PREDOMINANT' named entity 'GFP' named entity 'BODIPY' named entity 'REQUIRED' named entity 'ANESTHESIA' named entity 'QUO' named entity 'SYNTHESIS' named entity 'BUTTER' named entity 'PERIODS' named entity 'PLANE' named entity 'TAGGED' named entity 'LACTATION' named entity 'FORMATION'

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