About: A High-Throughput Single-Clone Phage Fluorescence Microwell Immunoassay and Laser-Driven Clonal Retrieval System

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About: A High-Throughput Single-Clone Phage Fluorescence Microwell Immunoassay and Laser-Driven Clonal Retrieval System Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A High-Throughput Single-Clone Phage Fluorescence Microwell Immunoassay and Laser-Driven Clonal Retrieval System
Creator	Chung, Junho Chang, Seohee Ha, Suji Han, Jerome Kim, Junhoi Kim, Soohyun Kwon, Sunghoon Lee, Daewon Lee, Hyunho Song, Seo
Source	PMC
abstract	Phage display is one of the most frequently used platform technologies utilized to screen and select therapeutic antibodies, and has contributed to the development of more than 10 therapeutic antibodies used in the clinic. Despite advantages like efficiency and low cost, it has intrinsic technical limitations, such as the asymmetrical amplification of the library after each round of biopanning, which is regarded as a reason for it yielding a very limited number of antigen binders. In this study, we developed a high-throughput single-clonal screening system comprised of fluorescence immunoassays and a laser-driven clonal DNA retrieval system using microchip technology. Using this system, from a single-chain variable fragment (scFv) library displayed on phages with a complexity of 5.21 × 10(5) harboring random mutations at five amino acid residues, more than 70,000 clones—corresponding to ~14% of the library complexity—were screened, resulting in 78 antigen-reactive scFv sequences with mutations restricted to the randomized residues. Our results demonstrate that this system can significantly reduce the number of biopanning rounds, or even eliminate the need for this process for libraries with lower complexity, providing an opportunity to obtain more diverse clones from the library.
has issue date	2020-03-29(xsd:dateTime)
bibo:doi	10.3390/biom10040517
bibo:pmid	32235304
has license	cc-by
sha1sum (hex)	23c22dd2965a1bab6a093c406116ae576a9582fc
schema:url	https://doi.org/10.3390/biom10040517
resource representing a document's title	A High-Throughput Single-Clone Phage Fluorescence Microwell Immunoassay and Laser-Driven Clonal Retrieval System
has PubMed Central identifier	PMC7226094
has PubMed identifier	32235304
schema:publication	Biomolecules
resource representing a document's body	covid:23c22dd2965a1bab6a093c406116ae576a9582fc#body_text
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