About: DDX1 is an RNA-dependent ATPase Involved in HIV-1 Rev Function and Virus Replication

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	DDX1 is an RNA-dependent ATPase Involved in HIV-1 Rev Function and Virus Replication
Creator	Ambrus-Aikelin, Geza Carmel, Andrew Edgcomb, Stephen Gerace, Larry Naji, Souad Reyes, Jason Saphire, Andrew Williamson, James
Source	Elsevier; Medline; PMC
abstract	The HIV-1 Rev protein is essential for the virus because it promotes nuclear export of alternatively-processed mRNAs, and Rev is also linked to translation of viral mRNAs and genome encapsidation. Previously, the human DEAD-box helicase DDX1 was suggested to be involved in Rev functions, but this relationship is not well understood. Biochemical studies of DDX1 and its interactions with Rev and model RNA oligonucleotides were carried out to investigate the molecular basis for association of these components. A combination of gel-filtration chromatography and circular dichroism spectroscopy demonstrated that recombinant DDX1 expressed in E. coli is a well-behaved folded protein. Binding assays using fluorescently-labeled Rev and cell-based immunoprecipitation analysis confirmed a specific RNA-independent DDX1-Rev interaction. Additionally, DDX1 was shown to be an RNA-activated ATPase, wherein Rev-bound RNA was equally effective at stimulating ATPase activity as protein-free RNA. Gel mobility shift assays further demonstrated that DDX1 forms complexes with Rev-bound RNA. RNA silencing of DDX1 provided strong evidence that DDX1 is required for both Rev activity and HIV production from infected cells. Collectively, these studies demonstrate a clear link between DDX1 and HIV-1 Rev in cell based assays of HIV-1 production, and provide the first demonstration that recombinant DDX1 binds Rev and RNA, and has RNA dependent catalytic activity.
has issue date	2012-01-01(xsd:dateTime)
bibo:doi	10.1016/j.jmb.2011.10.032
bibo:pmid	22051512
has license	green-oa
sha1sum (hex)	231377128610c5776532830eb8eb8d79f17fb120
schema:url	https://doi.org/10.1016/j.jmb.2011.10.032
resource representing a document's title	DDX1 is an RNA-dependent ATPase Involved in HIV-1 Rev Function and Virus Replication
has PubMed Central identifier	PMC3249508
has PubMed identifier	22051512
schema:publication	Journal of Molecular Biology
resource representing a document's body	covid:231377128610c5776532830eb8eb8d79f17fb120#body_text
is schema:about of	named entity 'well-behaved' named entity 'equally' named entity 'demonstration' named entity 'provide' named entity 'HIV' named entity 'complexes' named entity 'carried' named entity 'RNA' named entity 'Biochemical' named entity 'PROVIDE' named entity 'EXPRESSED' named entity 'COMPONENTS' named entity 'VIRUS REPLICATION' named entity 'BASED' named entity 'NOT WELL' named entity 'MODEL' named entity 'FILTRATION' named entity 'FORMS' named entity 'REV PROTEIN' named entity 'INTERACTIONS' named entity 'MRNAS' named entity 'FREE' named entity 'FOLDED' named entity 'TO INVESTIGATE' named entity 'USING' named entity 'BOUND' covid:arg/231377128610c5776532830eb8eb8d79f17fb120 named entity 'BINDS' named entity 'DEAD-BOX' named entity 'DEMONSTRATION' named entity 'VIRAL' named entity 'ACTIVITY' named entity 'HIV-1' named entity 'LABELED' named entity 'EVIDENCE' named entity 'CLEAR' named entity 'GENOME' named entity 'E. COLI' named entity 'STIMULATING' named entity 'THESE' named entity 'CONFIRMED' named entity 'HIV' named entity 'TRANSLATION' named entity 'INTERACTION' named entity 'ENCAPSIDATION' named entity 'MOLECULAR BASIS' named entity 'CHROMATOGRAPHY' named entity 'ESSENTIAL' named entity 'PRODUCTION' named entity 'COMBINATION' named entity 'ANALYSIS' named entity 'LINK' named entity 'FUNCTIONS' named entity 'RNA-DEPENDENT ATPASE' named entity 'HIV-1' named entity 'RNA SILENCING' named entity 'DDX1' named entity 'FUNCTION' named entity 'IMMUNOPRECIPITATION' named entity 'STRONG' named entity 'REV' named entity 'COMPLEXES' named entity 'INFECTED' named entity 'ATPASE ACTIVITY' named entity 'INVOLVED' named entity 'STUDIES' named entity 'BINDING ASSAYS' named entity 'EFFECTIVE' named entity 'BUT' named entity 'SPECIFIC' named entity 'CIRCULAR DICHROISM SPECTROSCOPY' named entity 'DEPENDENT' named entity 'VIRUS' named entity 'ATPASE'

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