About: Grouper interferon-induced transmembrane protein 3 (IFITM3) inhibits the infectivity of iridovirus and nodavirus by restricting viral entry

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About: Grouper interferon-induced transmembrane protein 3 (IFITM3) inhibits the infectivity of iridovirus and nodavirus by restricting viral entry Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Grouper interferon-induced transmembrane protein 3 (IFITM3) inhibits the infectivity of iridovirus and nodavirus by restricting viral entry
Creator	Huang, Xiaohong Huang, Youhua Qin, Qiwei Wang, Liqun Huang, Liwei Huang, Zhang Zhang, Ya Zheng, Jiaying
Source	Elsevier; Medline; PMC
abstract	Interferon-induced transmembrane proteins (IFITMs) have been identified as important host restriction factors in mammals for the control of infection by multiple viruses. However, the antiviral functions of IFITMs against fish viruses remain largely uncertain. In this study, the IFITM3 homolog from orange spotted grouper (EcIFITM3) was cloned and its roles in grouper virus infection were investigated. The full-length cDNA of EcIFITM3 was 737 bp, which was composed of a 16 bp 5′-UTR, a 274 bp 3′-UTR, and a 447 bp ORF. EcIFITM3 encodes a 148-amino-acid polypeptide, which contains five domains, i.e., the N-terminal domain (aa 1–65), TM1 (aa 66–90), the cytoplasmic domain (aa 91–110), TM2 (aa 111–140), and the C-terminal domain (aa 141–148), and shares 78% and 47% identity with IFITM3 of gilthead seabream (Sparus aurata) and human (Homo sapiens), respectively. EcIFITM3 mRNA was detected in 12 tissues of healthy groupers, with the highest expression levels in the head kidney. Additionally, the in vitro mRNA levels of EcIFITM3 were significantly upregulated by infection with Singapore grouper iridovirus (SGIV) or red spotted grouper nervous necrosis virus (RGNNV), or treatment with polyinosinic-polycytidylic acid (poly I:C) or lipopolysaccharide (LPS). Subcellular localization analysis showed that EcIFITM3 was mainly distributed in the cell membrane of grouper cells. In vitro, the ectopic expression of EcIFITM3 inhibited SGIV and RGNNV infection, as demonstrated by the reduced severity of the cytopathic effect, decreased virus production, and low levels of viral mRNA and proteins. Consistently, knockdown of EcIFITM3 by small interfering RNAs (siRNAs) enhanced SGIV and RGNNV replication. EcIFITM3 overexpression and knockdown experiments both suggested that EcIFITM3 inhibits the infection of SGIV and RGNNV by restricting virus entry.
has issue date	2020-06-10(xsd:dateTime)
bibo:doi	10.1016/j.fsi.2020.06.001
bibo:pmid	32531330
has license	no-cc
sha1sum (hex)	22ef897741339fde1df942b41132532a988f9a89
schema:url	https://doi.org/10.1016/j.fsi.2020.06.001
resource representing a document's title	Grouper interferon-induced transmembrane protein 3 (IFITM3) inhibits the infectivity of iridovirus and nodavirus by restricting viral entry
has PubMed Central identifier	PMC7283088
has PubMed identifier	32531330
schema:publication	Fish Shellfish Immunol
resource representing a document's body	covid:22ef897741339fde1df942b41132532a988f9a89#body_text
is schema:about of	named entity 'polypeptide' named entity 'poly' named entity 'reduced' named entity 'remain' named entity 'inhibits' named entity '3'-UTR' named entity 'necrosis' named entity 'experiments' named entity 'cells' named entity 'transmembrane protein' named entity 'inhibits' named entity 'DOI' named entity 'IMPORTANT' named entity 'STUDY' named entity 'TM2' named entity 'INFECTIVITY' named entity 'FUNCTIONS' named entity 'TRANSMEMBRANE PROTEINS' named entity 'GILTHEAD SEABREAM' named entity 'virus' named entity 'virus' named entity 'Subcellular localization' named entity 'lipopolysaccharide' named entity 'antiviral' named entity 'siRNAs' named entity 'encodes' named entity 'mammals' named entity 'mRNA' named entity 'proteins' named entity 'inhibits' named entity 'grouper' named entity 'nodavirus' named entity 'spotted' named entity 'spotted' named entity 'Fish' named entity 'nodavirus' named entity 'mammals' named entity 'antiviral' named entity 'LPS' named entity 'mRNA' named entity 'virus entry' named entity 'IFITM3' named entity 'grouper' named entity 'transmembrane proteins' named entity 'interferon' named entity 'Immunology' named entity 'gilthead seabream' named entity 'C-terminal' named entity '3'-UTR' named entity 'siRNAs' named entity 'virus' named entity 'infection' named entity 'N-terminal' named entity 'upregulated' named entity 'Singapore' named entity 'viruses' named entity 'infection' named entity 'iridovirus' named entity 'transmembrane protein' named entity 'interferon' named entity 'iridovirus' named entity 'cytopathic' named entity 'knockdown' named entity 'IFITM5' named entity 'ImageJ' named entity 'transfected' named entity 'transmembrane domains' named entity 'virus entry' named entity 'LPS' named entity 'qPCR' named entity 'paraformaldehyde' named entity 'Toyobo' named entity 'total protein' named entity 'virus replication'

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