About: Cytokine pre-activation of cryopreserved xenogeneic-free human mesenchymal stromal cells enhances resolution and repair following ventilator-induced lung injury potentially via a KGF-dependent mechanism

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About: Cytokine pre-activation of cryopreserved xenogeneic-free human mesenchymal stromal cells enhances resolution and repair following ventilator-induced lung injury potentially via a KGF-dependent mechanism Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Cytokine pre-activation of cryopreserved xenogeneic-free human mesenchymal stromal cells enhances resolution and repair following ventilator-induced lung injury potentially via a KGF-dependent mechanism
Creator	Laffey, John Horie, Shahd O'toole, Daniel Barry, Frank Gaynard, Sean Murphy, Mary Scully, Michael
Source	PMC
abstract	BACKGROUND: Human mesenchymal stem/stromal cells (hMSCs) represent a promising therapeutic strategy for ventilator-induced lung injury (VILI) and acute respiratory distress syndrome. Translational challenges include restoring hMSC efficacy following cryopreservation, developing effective xenogeneic-free (XF) hMSCs and establishing true therapeutic potential at a clinically relevant time point of administration. We wished to determine whether cytokine pre-activation of cryopreserved, bone marrow-derived XF-hMSCs would enhance their capacity to facilitate injury resolution following VILI and elucidate mechanisms of action. METHODS: Initially, in vitro studies examined the potential for the secretome from cytokine pre-activated XF-hMSCs to attenuate pulmonary epithelial injury induced by cyclic mechanical stretch. Later, anaesthetised rats underwent VILI and, 6 h following injury, were randomized to receive 1 × 10(7) XF-hMSC/kg that were (i) naive fresh, (ii) naive cryopreserved, (iii) cytokine pre-activated fresh or (iv) cytokine pre-activated cryopreserved, while control animals received (v) vehicle. The extent of injury resolution was measured at 24 h after injury. Finally, the role of keratinocyte growth factor (KGF) in mediating the effect of pre-activated XF-hMSCs was determined in a pulmonary epithelial wound repair model. RESULTS: Pre-activation enhanced the capacity of the XF-hMSC secretome to decrease stretch-induced pulmonary epithelial inflammation and injury. Both pre-activated fresh and cryopreserved XF-hMSCs enhanced resolution of injury following VILI, restoring oxygenation, improving lung compliance, reducing lung leak and improving resolution of lung structural injury. Finally, the secretome of pre-activated XF-hMSCs enhanced epithelial wound repair, in part via a KGF-dependent mechanism. CONCLUSIONS: Cytokine pre-activation enhanced the capacity of cryopreserved, XF-hMSCs to promote injury resolution following VILI, potentially via a KGF-dependent mechanism.
has issue date	2020-02-05(xsd:dateTime)
bibo:doi	10.1186/s40635-020-0295-5
bibo:pmid	32025852
has license	cc-by
sha1sum (hex)	229c14218c264e64c209ba8022c636d52fe8f279
schema:url	https://doi.org/10.1186/s40635-020-0295-5
resource representing a document's title	Cytokine pre-activation of cryopreserved xenogeneic-free human mesenchymal stromal cells enhances resolution and repair following ventilator-induced lung injury potentially via a KGF-dependent mechanism
has PubMed Central identifier	PMC7002627
has PubMed identifier	32025852
schema:publication	Intensive Care Med Exp
resource representing a document's body	covid:229c14218c264e64c209ba8022c636d52fe8f279#body_text
is schema:about of	named entity 'injury' named entity 'mechanical stretch' named entity 'Results' named entity 'wound repair' named entity 'receive' named entity 'ACTIVATED' named entity 'ENHANCED' named entity 'METHODS' named entity 'EFFECTIVE' named entity 'DEPENDENT' named entity 'FRESH' named entity 'ACTION' named entity 'HMSC' named entity 'REDUCING' named entity 'DEVELOPING' named entity 'EFFICACY' named entity 'CELLS' named entity 'developing' named entity 'Both' named entity 'efficacy' named entity 'represent' named entity 'Human' named entity 'measured' named entity 'fresh' named entity 'therapeutic' named entity 'growth factor' named entity 'epithelial' named entity 'therapeutic' named entity 'VILI' named entity 'mesenchymal' named entity 'potential' named entity 'promising' named entity 'cryopreserved' named entity 'time point' named entity 'mechanism' named entity 'mesenchymal' named entity 'KGF' named entity 'epithelial' named entity 'wound repair' named entity 'cryopreserved' named entity 'lung' named entity 'VILI' named entity 'KGF' named entity 'VILI' named entity 'keratinocyte growth factor' named entity 'bone marrow' named entity 'acute respiratory distress syndrome' named entity 'injury' named entity 'cryopreservation' named entity 'hypoxia' named entity 'cryopreserved' named entity 'cryopreserved' named entity 'cryopreserved' named entity 'MSCs' named entity 'lung' named entity 'fibroblasts' named entity 'xenogeneic' named entity 'luciferase' named entity 'anaesthesia' named entity 'cryopreserved' named entity 'cryopreservation' named entity 'Burlington, NC' named entity 'virus' named entity 'immunogenicity' named entity 'interferon-γ' named entity 'cytokine' named entity 'microenvironment' named entity 'Cytokine' named entity 'Acute respiratory distress syndrome' named entity 'xenogeneic' named entity 'cryopreserved' named entity 'Laryngoscopy' named entity 'NF-κB' named entity 'Cytokine' named entity 'intravenous access'

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