About: Production of specific IgY antibody to the recombinant FanC protein produced in Escherichia coli

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About: Production of specific IgY antibody to the recombinant FanC protein produced in Escherichia coli Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Production of specific IgY antibody to the recombinant FanC protein produced in Escherichia coli
Creator	Haghparast, Alireza Nasiri, Khadijeh Nassiri, Mohammadreza Tahmoorespur, Mojtaba Zibaee, Saeed
Source	Medline; PMC
abstract	OBJECTIVE(S): Enterotoxigenic Escherichia coli (ETEC) strains are one of the primary causes of diarrhea in newborn calves and in humans, pigs, and sheep. IgY technology has been identified as a promising alternative to generating a mass amount of specific antibody for use in immunotherapy and immunodiagnostics. The purpose of this study was to produce specific antibody by egg yolk antibody (IgY) to recombinant FanC protein from ETEC. MATERIALS AND METHODS: FanC (K99) gene was amplified from ETEC by specific primers and polymerase chain reaction. The gene was cloned and subcloned into pTZ57R/T and pET32a (+) vectors, respectively. Recombinant vector was transferred into E. coli BL21 CodonPlus (DE3). Protein expression was investigated by 1 mM IPTG induction. Hens were immunized by the purified recombinant FanC protein. The activity and specificity of the IgY antibody were detected by dot-blotting, Western blotting, and indirect ELISA. RESULTS: We obtained FanC specific IgYs by immunizing the hens with the recombinant FanC protein. The anti-FanC IgY showed binding specifically to the FanC protein of ETEC. CONCLUSION: The results emphasize that specific IgY against the recombinant FanC protein could be recommended as a candidate for passive immunization against ETEC infection in animals and humans.
has issue date	2016-08-01(xsd:dateTime)
bibo:pmid	27746871
has license	cc-by-nc-sa
sha1sum (hex)	2024ea5609babbd8769ee9f1898e825306b49cfe
resource representing a document's title	Production of specific IgY antibody to the recombinant FanC protein produced in Escherichia coli
has PubMed Central identifier	PMC5048125
has PubMed identifier	27746871
schema:publication	Iran J Basic Med Sci
resource representing a document's body	covid:2024ea5609babbd8769ee9f1898e825306b49cfe#body_text
is schema:about of	named entity 'ANTIBODY' named entity 'WESTERN BLOTTING' named entity 'ACTIVITY' named entity 'STUDY' named entity 'STRAINS' named entity 'INDUCTION' named entity 'PRIMERS' named entity 'IGY' named entity 'SPECIFIC ANTIBODY' named entity 'EGG YOLK' named entity 'IMMUNIZED' named entity 'PIGS' named entity 'RECOMBINANT' named entity 'E. coli' named entity 'promising' named entity 'recombinant' named entity 'recombinant' named entity 'specific' named entity 'produced' named entity 'IgY' named entity 'PROTEIN ' named entity 'FANC' named entity 'CLONED' named entity 'OBTAINED' named entity 'NEWBORN' named entity 'E. COLI' named entity 'A MASS' named entity 'IGY' named entity 'RECOMBINANT' named entity 'ENTEROTOXIGENIC ESCHERICHIA COLI' named entity 'HENS' named entity 'IDENTIFIED' named entity 'RECOMBINANT VECTOR' named entity 'BLOTTING' named entity 'ANTIBODY' named entity 'GENE' named entity 'CAUSES' named entity 'RESULTS' named entity 'IPTG' named entity 'PROTEIN' named entity 'AMPLIFIED' named entity 'POLYMERASE CHAIN REACTION' named entity 'BINDING' named entity 'DIARRHEA' named entity 'SPECIFIC' named entity 'DETECTED' named entity 'MATERIALS' named entity 'METHODS' named entity 'IMMUNOTHERAPY' named entity 'BL21' named entity 'FANC' named entity 'DOT' named entity 'IMMUNODIAGNOSTICS' named entity 'PRIMARY' named entity 'TO PRODUCE' named entity 'PRODUCTION' named entity 'SPECIFIC' named entity 'ESCHERICHIA COLI' named entity 'CALVES' named entity 'PROTEIN ' named entity 'SHEEP' named entity 'INDIRECT' named entity 'ALTERNATIVE' named entity 'PURIFIED' named entity 'USE' named entity 'HUMANS' named entity 'TRANSFERRED' named entity 'GENERATING' named entity 'TECHNOLOGY' named entity '1 MM' named entity 'INVESTIGATED' named entity 'SPECIFICITY' named entity 'PROTEIN EXPRESSION' named entity 'VECTORS' named entity 'PURPOSE' named entity 'ONE OF' named entity 'ELISA' covid:arg/2024ea5609babbd8769ee9f1898e825306b49cfe named entity 'investigated'

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