About: Discovery and Characterization of ZUFSP/ZUP1, a Distinct Deubiquitinase Class Important for Genome Stability

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About: Discovery and Characterization of ZUFSP/ZUP1, a Distinct Deubiquitinase Class Important for Genome Stability Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Discovery and Characterization of ZUFSP/ZUP1, a Distinct Deubiquitinase Class Important for Genome Stability
Creator	Kulathu, Yogesh Ahel, Ivan Gibbs-Seymour, Ian Kwasna, Dominika Natarajan, Jayaprakash Arif, Syed Rehman, Abdul
Source	PMC
abstract	Deubiquitinating enzymes (DUBs) are important regulators of ubiquitin signaling. Here, we report the discovery of deubiquitinating activity in ZUFSP/C6orf113. High-resolution crystal structures of ZUFSP in complex with ubiquitin reveal several distinctive features of ubiquitin recognition and catalysis. Our analyses reveal that ZUFSP is a novel DUB with no homology to any known DUBs, leading us to classify ZUFSP as the seventh DUB family. Intriguingly, the minimal catalytic domain does not cleave polyubiquitin. We identify two ubiquitin binding domains in ZUFSP: a ZHA (ZUFSP helical arm) that binds to the distal ubiquitin and an atypical UBZ domain in ZUFSP that binds to polyubiquitin. Importantly, both domains are essential for ZUFSP to selectively cleave K63-linked polyubiquitin. We show that ZUFSP localizes to DNA lesions, where it plays an important role in genome stability pathways, functioning to prevent spontaneous DNA damage and also promote cellular survival in response to exogenous DNA damage.
has issue date	2018-04-05(xsd:dateTime)
bibo:doi	10.1016/j.molcel.2018.02.023
bibo:pmid	29576527
has license	cc-by
sha1sum (hex)	1e4ac3a8108d17c0034aa38040a99be38819c12e
schema:url	https://doi.org/10.1016/j.molcel.2018.02.023
resource representing a document's title	Discovery and Characterization of ZUFSP/ZUP1, a Distinct Deubiquitinase Class Important for Genome Stability
has PubMed Central identifier	PMC5896202
has PubMed identifier	29576527
schema:publication	Mol Cell
resource representing a document's body	covid:1e4ac3a8108d17c0034aa38040a99be38819c12e#body_text
is schema:about of	named entity 'cleaves' named entity 'mechanisms' named entity 'Important' named entity 'Graphical' named entity 'survival' named entity 'binding' named entity 'Abstract' named entity 'genome instability' named entity 'Genome Stability' named entity 'glycerol' named entity 'replication stress' named entity 'cloned' named entity 'DNA damage' named entity 'UFM1' named entity 'DSB' named entity 'zebrafish' named entity 'camptothecin' named entity 'DNA lesions' named entity 'C2H2' named entity 'doxycycline' named entity 'binding sites' named entity 'HPO' named entity 'biochemical' named entity 'Confocal microscopy' named entity 'alpha helix' named entity 'hydrogen bonding' named entity 'RMSD' named entity 'distal' named entity 'C360' named entity 'nuclease' named entity 'metalloproteases' named entity 'UFM1' named entity 'D512' named entity 'Silver stain' named entity 'bovine' named entity 'peptidase' named entity 'flash frozen' named entity 'cell cycle' named entity 'human disease' named entity 'N-terminal' named entity 'molecule' named entity 'NP-40' named entity 'immunoprecipitation' named entity 'benzamidine' named entity 'chicken' named entity 'catalytic domain' named entity 'molar ratio' named entity 'motif' named entity 'resin' named entity 'digestion' named entity 'replication stress' named entity 'catalytic domain' named entity '53BP1' named entity 'RPA1' named entity 'phenol' named entity 'distal' named entity 'leucine' named entity 'Sequence alignment' named entity 'PCNA' named entity 'aliphatic' named entity 'clastogen' named entity 'RPA3' named entity 'sonicated' named entity 'wild-type' named entity 'response to DNA damage' named entity 'RPA2' named entity 'protein' named entity 'DNA Polymerase' named entity 'DNA repair' named entity 'Dionex' named entity 'mass spectrometry' named entity 'genome stability' named entity 'immunostaining' named entity 'irradiation' named entity 'Thermo Fisher'

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