About: 16S ribosomal RNA sequencing reveals a modulation of intestinal microbiome and immune response by dietary L-theanine supplementation in broiler chickens

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About: 16S ribosomal RNA sequencing reveals a modulation of intestinal microbiome and immune response by dietary L-theanine supplementation in broiler chickens Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	16S ribosomal RNA sequencing reveals a modulation of intestinal microbiome and immune response by dietary L-theanine supplementation in broiler chickens
Creator	Saeed, Muhammad Chao, Sun Qian, Ren Tiantian, Zhang Yatao, Xu
Source	PMC
abstract	Despite the availability of abundant literature on green tea, studies on the use of L-theanine (an amino acid found only in green tea) as a feed additive in poultry especially broiler are limited. So, this study was conducted to explore the effects of L-theanine on the intestinal microbiome and immune response in a broiler. A total of 400-d-old chicks were randomly divided into four treatment groups (A, B, C, and D) using a complete randomized design. Treatments were as follows: A, control (basal diet); B, basal diet + 100 mg L-theanine/kg diet; C, basal diet + 200 mg L-theanine/kg diet; and D, basal diet + 300 mg L-theanine/kg diet. Mucosal samples from ileum and jejunum of broiler chicken were extracted at 21 and 42 d of age. Extraction of genomic DNA was followed by amplification of V3 and V4 hypervariable regions of 16S ribosomal RNA. After Illumina sequencing, results revealed that treatment with L-theanine significantly increased the population of Lactobacillus in ileum and jejunum as compared to a control group, but the higher population was observed in jejunum at both 21 and 42 d of age. The overall diversity of the jejunum microbiome in the treatment group was significantly lower than that of the ileum and control group (P < 0.05). Results of this study revealed that mRNA expression of TLRs (TLR-2 and TLR-4) and cytokines (TNF-α, IFN-γ, and IL-2) was decreased in response to treatment with L-theanine. Moreover, the negative correlation of abundance of Lactobacillus was observed with expression of IL-2 and IFN-γ in the intestine and these effects were highly significant (P < 0.01). In summary, our finding revealed that dietary supplementation of L-theanine exhibited a positive influence on intestinal bacteria by supporting beneficial microbes like Lactobacillus while decreasing harmful microbes like Clostridium.
has issue date	2018-08-30(xsd:dateTime)
bibo:doi	10.3382/ps/pey394
bibo:pmid	30169691
has license	no-cc
sha1sum (hex)	1db02ff76c1ebaa9aa5ed0a472c069f81174b77b
schema:url	https://doi.org/10.3382/ps/pey394
resource representing a document's title	16S ribosomal RNA sequencing reveals a modulation of intestinal microbiome and immune response by dietary L-theanine supplementation in broiler chickens
has PubMed Central identifier	PMC7107316
has PubMed identifier	30169691
schema:publication	Poult Sci
resource representing a document's body	covid:1db02ff76c1ebaa9aa5ed0a472c069f81174b77b#body_text
is schema:about of	named entity 'DIETARY' named entity '16S ribosomal RNA' named entity 'Lactobacillus' named entity 'ribosomal RNA' named entity 'RNA sequencing' named entity 'RIBOSOMAL RNA' named entity 'HIGHLY SIGNIFICANT' named entity 'CHICKS' named entity 'LITERATURE' named entity 'THESE' named entity 'HARMFUL' named entity 'COMPLETE' named entity 'REGIONS' named entity 'TLR-4' named entity 'ABUNDANCE' named entity 'BROILER' named entity 'SAMPLES' named entity 'TREATMENT GROUP' named entity 'STUDY' named entity 'EFFECTS' named entity '200' named entity 'RANDOMIZED DESIGN' named entity 'MRNA EXPRESSION' named entity 'BACTERIA ' named entity 'OBSERVED' named entity 'OLD' named entity 'IFN' named entity 'AGE' named entity 'FOLLOWS' named entity 'ILLUMINA SEQUENCING' named entity 'DECREASED' named entity 'EXPRESSION' named entity 'FEED ADDITIVE' named entity 'COMPARED' named entity 'REVEALED' named entity 'GENOMIC DNA' named entity 'FINDING' named entity 'DIVIDED' named entity 'LIKE' named entity '28P' named entity 'RESULTS' named entity 'CLOSTRIDIUM' named entity 'INCREASED' named entity 'TREATMENTS' named entity '28A' named entity 'FOLLOWED BY' named entity 'SUMMARY' named entity 'INTESTINE' named entity 'INTESTINAL MICROBIOME' named entity 'RNA SEQUENCING' named entity 'HIGHER' named entity 'FOUND' named entity 'AVAILABILITY OF' named entity 'L-THEANINE' named entity 'GREEN TEA' named entity '16S RIBOSOMAL RNA' named entity 'LOWER' named entity 'DIVERSITY' named entity 'LIMITED' named entity 'POPULATION' named entity '0.05' named entity 'USING' named entity 'ILEUM' named entity 'TREATMENT' named entity 'RESPONSE TO TREATMENT' named entity 'BUT' named entity 'BROILER CHICKEN' named entity 'USE OF' named entity 'AMINO ACID' named entity 'CONTROL GROUP' named entity 'MODULATION' named entity 'OVERALL' named entity 'EXTRACTION' named entity 'L-THEANINE' named entity '300' named entity 'BROILER' named entity 'IMMUNE RESPONSE'

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