About: Development of an Immunochromatographic Strip for Rapid Detection of Canine Adenovirus

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About: Development of an Immunochromatographic Strip for Rapid Detection of Canine Adenovirus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Development of an Immunochromatographic Strip for Rapid Detection of Canine Adenovirus
Creator	Ge, Jinying Cai, Xuehui Wang, Shujie An, Tongqing Duan, Guixin Li, Xi Wen, Yongjun Yang, Kongbin Kroon, Erna Alexandre, India Machado, De Sun, Mingxia
topic	covid:1d105041da2d3a6e6e16cd0dce1f0a672066d407#this
Source	Medline; PMC
abstract	Although canine adenovirus (CAdV) is highly prevalent in dogs, there is currently a lack of a quick diagnostic method. In this study, we developed a rapid immunochromatographic strip (ICS) assay using colloidal gold coupled to CAdV-2-specific monoclonal antibodies (mAbs). BALB/c mice were immunized with a purified CAdV-2 suspension, and four mAbs (belonging to two different epitopes) were generated and designated as 2C1, 7D7, 10D1, and 4G1. Western blot and protein spectral analysis indicated that the hexon protein of CAdV-2 recognized all four mAbs. The colloidal gold-coupled 7D7 and 2C1 mAbs were chosen for inclusion in the rapid ICS assay. The optimal concentrations of the coating antibody (2C1), the capture antibody (7D7), and the goat anti-mouse antibody were 1.0 mg/ml, 10 μg/ml, and 2.0 mg/ml, respectively. The limit of detection was approximately 2.0 × 10(2) tissue culture infective dose (TCID(50))/ml. Other common canine viruses were tested to evaluate the specificity of the ICS, and positive results were observed for only CAdV-1 and CAdV-2. The ICS test was conducted on 360 samples to detect CAdV, and the results were compared with those of polymerase chain reaction (PCR) tests. The ICS test was found to be a sufficiently sensitive and specific detection method for the convenient and rapid detection of CAdV.
has issue date	2019-12-11(xsd:dateTime)
bibo:doi	10.3389/fmicb.2019.02882
bibo:pmid	31921060
has license	cc-by
sha1sum (hex)	1d105041da2d3a6e6e16cd0dce1f0a672066d407
schema:url	https://doi.org/10.3389/fmicb.2019.02882
resource representing a document's title	Development of an Immunochromatographic Strip for Rapid Detection of Canine Adenovirus
has PubMed Central identifier	PMC6917642
has PubMed identifier	31921060
schema:publication	Front Microbiol
resource representing a document's body	covid:1d105041da2d3a6e6e16cd0dce1f0a672066d407#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/1d105041da2d3a6e6e16cd0dce1f0a672066d407
is schema:about of	named entity 'evaluate' named entity 'rapid' named entity 'mAbs' named entity 'The limit' named entity 'DOGS' named entity 'ASSAY' named entity 'COMPARED' named entity 'CONVENIENT' named entity 'ICS' named entity 'SAMPLES' named entity 'INFECTIVE DOSE' named entity 'SPECIFICITY' named entity 'COATING' named entity 'DIFFERENT' named entity 'EPITOPES' named entity 'CANINE' named entity 'TISSUE CULTURE' named entity 'DESIGNATED' named entity 'MOUSE' named entity 'DETECTION METHOD' named entity 'POLYMERASE CHAIN REACTION' named entity 'COMMON' named entity 'PREVALENT' named entity 'PURIFIED' named entity 'GOAT' named entity '360' named entity 'DETECTION' named entity 'PROTEIN ' named entity 'USING' named entity 'MONOCLONAL ANTIBODIES' named entity 'BALB' named entity 'LACK' named entity 'OPTIMAL' named entity 'CANINE ADENOVIRUS' named entity 'VIRUSES' named entity 'DIAGNOSTIC METHOD' named entity 'RAPID' named entity 'WESTERN BLOT' named entity 'COUPLED' named entity 'MICE' named entity 'DETECTION' named entity 'RAPID' named entity 'TO DETECT' named entity 'LIMIT OF DETECTION' named entity 'CAPTURE' named entity 'SENSITIVE' named entity 'CITATION' named entity 'FOUND' named entity 'CANINE ADENOVIRUS' named entity 'STRIP' named entity 'DEVELOPMENT' named entity 'COLLOIDAL GOLD' named entity 'HIGHLY' named entity 'ANTIBODY' named entity 'CONCENTRATIONS' named entity 'MG%' named entity 'IMMUNIZED' named entity 'SPECIFIC' named entity 'TESTED' named entity 'CURRENTLY' named entity 'GENERATED' named entity 'SPECTRAL ANALYSIS' named entity 'TEST' named entity 'POSITIVE' named entity 'HEXON PROTEIN' named entity 'APPROXIMATELY' named entity 'OBSERVED' named entity 'SUSPENSION'

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