About: Competing endogenous RNA network profiling reveals novel host dependency factors required for MERS-CoV propagation

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About: Competing endogenous RNA network profiling reveals novel host dependency factors required for MERS-CoV propagation Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Competing endogenous RNA network profiling reveals novel host dependency factors required for MERS-CoV propagation
Creator	Chan, Jasper Fuk-Woo Yuen, Kwok-Yung Zhang, Xi Chu, Hin Wang, Yixin Yang, Dong Zhu, Zheng Shuai, Huiping Wen, Lei Yuan, Shuofeng Yin, Feifei Hou, Yuxin
Source	Medline; PMC
abstract	Circular RNAs (circRNAs) are an integral component of the host competitive endogenous RNA (ceRNA) network. These noncoding RNAs are characterized by their unique splicing reactions to form covalently closed loop structures and play important RNA regulatory roles in cells. Recent studies showed that circRNA expressions were perturbed in viral infections and circRNAs might serve as potential antiviral targets. We investigated the host ceRNA network changes and biological relevance of circRNAs in human lung adenocarcinoma epithelial (Calu-3) cells infected with the highly pathogenic Middle East respiratory syndrome coronavirus (MERS-CoV). A total of ≥49337 putative circRNAs were predicted. Among the 7845 genes which generated putative circRNAs, 147 (1.9%) of them each generated ≥30 putative circRNAs and were involved in various biological, cellular, and metabolic processes, including viral infections. Differential expression (DE) analysis showed that the proportion of DE circRNAs significantly (P < 0.001) increased at 24 h-post infection. These DE circRNAs were clustered into 4 groups according to their time-course expression patterns and demonstrated inter-cluster and intra-cluster variations in the predicted functions of their host genes. Our comprehensive circRNA-miRNA-mRNA network identified 7 key DE circRNAs involved in various biological processes upon MERS-CoV infection. Specific siRNA knockdown of two selected DE circRNAs (circFNDC3B and circCNOT1) significantly reduced MERS-CoV load and their target mRNA expression which modulates various biological pathways, including the mitogen-activated protein kinase (MAPK) and ubiquitination pathways. These results provided novel insights into the ceRNA network perturbations, biological relevance of circRNAs, and potential host-targeting antiviral strategies for MERS-CoV infection.
has issue date	2020-03-30(xsd:dateTime)
bibo:doi	10.1080/22221751.2020.1738277
bibo:pmid	32223537
has license	cc-by
sha1sum (hex)	1cd1be88f8787d09aaf55dcc91418401b9965c72
schema:url	https://doi.org/10.1080/22221751.2020.1738277
resource representing a document's title	Competing endogenous RNA network profiling reveals novel host dependency factors required for MERS-CoV propagation
has PubMed Central identifier	PMC7170352
has PubMed identifier	32223537
schema:publication	Emerg Microbes Infect
resource representing a document's body	covid:1cd1be88f8787d09aaf55dcc91418401b9965c72#body_text
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