About: Development of a Sensitive Real-Time Fast-qPCR Based on SYBR(®) Green for Detection and Quantification of Chicken Parvovirus (ChPV)

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About: Development of a Sensitive Real-Time Fast-qPCR Based on SYBR(®) Green for Detection and Quantification of Chicken Parvovirus (ChPV) Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Development of a Sensitive Real-Time Fast-qPCR Based on SYBR(®) Green for Detection and Quantification of Chicken Parvovirus (ChPV)
Creator	Astolfi-Ferreira, Claudete Ferreira, Antonio Nuñez, Luis De La Torre, David Buim, Chaible, Lucas Dagli, Zaidan Id, Marcos Id, Silvana Lucia, Maria Murakami, Alexandre Santander-Parra,
Source	Medline; PMC
abstract	Many viruses have been associated with runting and stunting syndrome (RSS). These viral infections mainly affect young chickens, causing apathy, depression, ruffled feathers, cloacal pasting, and diarrhea. Chicken Parvovirus (ChPV) is such an infection and has been detected in chickens showing signs of enteric diseases worldwide. Therefore, the present study aims to develop a sensitive real-time fast-qPCR assay based on SYBR(®) Green for detection and quantification of ChPV. A 561-bp non-structural (NS) gene was amplified and cloned, and a pair of primers was designed based on conserved nucleotide sequences on the NS gene of ChPV, the intercalating DNA reagent SYBR(®) Green was employed, and the Fast mode of a thermocycler was used. The assay detects 10(9) to 10(1) copies of the genome (CG). The limit of detection (LoD) was estimated to five CG, and the limit of quantification (LoQ) was estimated at ten CG. The standard curve efficiency was 101.94%, and the melting curve showed a unique clean peak and a melting temperature of 79.3 °C. The assay was specific to amplify the ChPV NS gene, and no amplification was shown from other viral genomes or in the negative controls. A total of 141 samples were tested using the assay, of which 139 samples were found positive. The highest CG value of ChPV was 5.7 × 10(6) CG/uL of DNA without apparent clinical signs of enteric disturbance, and 4.6 × 10(6) CG/uL DNA were detected in chickens with RSS.
has issue date	2018-07-25(xsd:dateTime)
bibo:doi	10.3390/vetsci5030069
bibo:pmid	30044371
has license	cc-by
sha1sum (hex)	1c8b4359eb1664ace34491b269090856fced85c9
schema:url	https://doi.org/10.3390/vetsci5030069
resource representing a document's title	Development of a Sensitive Real-Time Fast-qPCR Based on SYBR(®) Green for Detection and Quantification of Chicken Parvovirus (ChPV)
has PubMed Central identifier	PMC6163237
has PubMed identifier	30044371
schema:publication	Vet Sci
resource representing a document's body	covid:1c8b4359eb1664ace34491b269090856fced85c9#body_text
is schema:about of	named entity 'DNA' named entity 'infection' named entity 'chickens' named entity 'tested' named entity 'unique' named entity 'copies' named entity 'detects' named entity 'amplify' covid:arg/1c8b4359eb1664ace34491b269090856fced85c9 named entity 'primers' named entity 'real-time' named entity 'apparent' named entity 'quantification' named entity 'SYBR' named entity 'depression' named entity 'feathers' named entity 'employed' named entity 'detected' named entity 'standard curve' named entity 'assay' named entity 'controls' named entity 'diseases' named entity 'enteric diseases' named entity 'qPCR' named entity 'apathy' named entity 'assay' named entity 'RSS' named entity 'limit of detection' named entity 'clinical signs' named entity 'nucleotide sequences' named entity 'Chicken' named entity 'qPCR' named entity '2.1' named entity 'qPCR' named entity 'highly conserved' named entity '3, 4' named entity 'genomes' named entity 'genotypes' named entity 'United States' named entity 'PCR' named entity 'states of Brazil' named entity 'VP1' named entity 'conserved region' named entity 'stunting' named entity 'United States' named entity 'LoQ' named entity 'Carlsbad, CA' named entity 'China' named entity 'chicken' named entity 'genome' named entity 'Korea' named entity '88.9' named entity 'sanitation' named entity 'recombinant' named entity 'gene' named entity 'negative stain' named entity 'GenBank' named entity 'Croatia' named entity 'PBS' named entity 'denaturation' named entity 'Poland' named entity 'Croatia' named entity 'PCR amplification' named entity 'qPCR' named entity 'Thermo Fisher Scientific' named entity 'Thermo Fisher' named entity 'thymus' named entity 'Geneious' named entity 'gene' named entity 'clinical signs'

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