About: A sensitive array-based assay for identifying multiple TMPRSS2:ERG fusion gene variants

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About: A sensitive array-based assay for identifying multiple TMPRSS2:ERG fusion gene variants Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A sensitive array-based assay for identifying multiple TMPRSS2:ERG fusion gene variants
Creator	Liu, Yu-Tsueng Lu, Qing Christensen, Kimberly Downs, Tracy Lin, Chunrun Nunez, Esperanza Wang-Rodriguez, Jessica Carson, Dennis
Source	PMC
abstract	Studies of gene fusions in solid tumors are not as extensive as in hematological malignancies due to several technical and analytical problems associated with tumor heterogeneity. Nevertheless, there is a growing interest in the role of fusion genes in common epithelial tumors after the discovery of recurrent TMPRSS2:ETS fusions in prostate cancer. Among all of the reported fusion partners in the ETS gene family, TMPRSS2:ERG is the most prevalent one. Here, we present a simple and sensitive microarray-based assay that is able to simultaneously determine multiple fusion variants with a single RT–PCR in impure RNA specimens. The assay detected TMPRSS2:ERG fusion transcripts with a detection sensitivity of <10 cells in the presence of more than 3000 times excess normal RNA, and in primary prostate tumors having no >1% of cancer cells. The ability to detect multiple transcript variants in a single assay is critically dependent on both the primer and probe designs. The assay should facilitate clinical and basic studies for fusion gene screening in clinical specimens, as it can be readily adapted to include multiple gene loci.
has issue date	2008-09-15(xsd:dateTime)
bibo:doi	10.1093/nar/gkn585
bibo:pmid	18794177
has license	cc-by-nc
sha1sum (hex)	1a7676018fd73150a122c2afb1362324dbab4918
schema:url	https://doi.org/10.1093/nar/gkn585
resource representing a document's title	A sensitive array-based assay for identifying multiple TMPRSS2:ERG fusion gene variants
has PubMed Central identifier	PMC2582611
has PubMed identifier	18794177
schema:publication	Nucleic Acids Res
resource representing a document's body	covid:1a7676018fd73150a122c2afb1362324dbab4918#body_text
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