About: The N-terminal domains of FLASH and Lsm11 form a 2:1 heterotrimer for histone pre-mRNA 3’-end processing

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About: The N-terminal domains of FLASH and Lsm11 form a 2:1 heterotrimer for histone pre-mRNA 3’-end processing Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	The N-terminal domains of FLASH and Lsm11 form a 2:1 heterotrimer for histone pre-mRNA 3’-end processing
Creator	Chou, Chi-Yuan Tong, Liang Lin, Min-Han Dominski, Zbigniew Tripathy, Ashutosh Marzluff, William Shen Aik, Wei
Source	PMC
abstract	Unlike canonical pre-mRNAs, animal replication-dependent histone pre-mRNAs lack introns and are processed at the 3’-end by a mechanism distinct from cleavage and polyadenylation. They have a 3’ stem loop and histone downstream element (HDE) that are recognized by stem-loop binding protein (SLBP) and U7 snRNP, respectively. The N-terminal domain (NTD) of Lsm11, a component of U7 snRNP, interacts with FLASH NTD and these two proteins recruit the histone cleavage complex containing the CPSF-73 endonuclease for the cleavage reaction. Here, we determined crystal structures of FLASH NTD and found that it forms a coiled-coil dimer. Using solution light scattering, we characterized the stoichiometry of the FLASH NTD-Lsm11 NTD complex and found that it is a 2:1 heterotrimer, which is supported by observations from analytical ultracentrifugation and crosslinking.
has issue date	2017-10-11(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0186034
bibo:pmid	29020104
has license	cc-by
sha1sum (hex)	1a52cac849bdb5840d115cf513ec8cc63dc2d56c
schema:url	https://doi.org/10.1371/journal.pone.0186034
resource representing a document's title	The N-terminal domains of FLASH and Lsm11 form a 2:1 heterotrimer for histone pre-mRNA 3’-end processing
has PubMed Central identifier	PMC5636114
has PubMed identifier	29020104
schema:publication	PLoS One
resource representing a document's body	covid:1a52cac849bdb5840d115cf513ec8cc63dc2d56c#body_text
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