About: Computational prediction and identification of dengue virus-specific CD4+ T-cell epitopes

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About: Computational prediction and identification of dengue virus-specific CD4+ T-cell epitopes Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Computational prediction and identification of dengue virus-specific CD4+ T-cell epitopes
Creator	Fang, Dan-Yun Jiang, Li-Fang Yan, Hui-Jun Zhou, Jun-Mei Wen, Jin-Sheng
Source	Elsevier; Medline; PMC
abstract	Abstract In this study, we tried to identify dengue virus-specific CD4+ T-cell epitopes, which can induce PBMC (peripheral blood mononuclear cells) isolated from DF convalescent patients (dengue virus type 1 infection) to secrete IFN-γ. PBMC of DF convalescent patients were stimulated in vitro with dengue virus-derived peptides, which were prepared based on the prediction of dengue virus-specific CD4+ T-cell epitopes by using RANKpep online software. Subsequently, the frequency of IFN-γ producing T cells and percentage of IFN-γ+ CD4+ T cells were measured by using ELISPOT assay and ICS assay (intracellular cytokine straining), respectively. The positive response of PBMC by ELISPOT showed that the numbers of SFC (spots forming cells) ranged from 50 to 310 SFC/1×106 PBMC. The positive response of PBMC by ICS assay showed that the percentage of IFN-γ+ CD4+ T cells ranged from 0.03 to 0.27%. As a result, C45-57 (KLVMAFIAFLRFL), E396-408 (SSIGKMFEATARG), NS323-35 (YRILQRGLLGRSQ), and NS3141-155 (NREGKIVGLYGNGVV) were identified as dengue virus-specific CD4+ T-cell epitopes.
has issue date	2008-03-31(xsd:dateTime)
bibo:doi	10.1016/j.virusres.2007.10.010
bibo:pmid	18061300
has license	els-covid
sha1sum (hex)	19a5e1a53b1a9d36e3e1956da3c1c184a24979af
schema:url	https://doi.org/10.1016/j.virusres.2007.10.010
resource representing a document's title	Computational prediction and identification of dengue virus-specific CD4+ T-cell epitopes
has PubMed Central identifier	PMC7114202
has PubMed identifier	18061300
schema:publication	Virus Research
resource representing a document's body	covid:19a5e1a53b1a9d36e3e1956da3c1c184a24979af#body_text
is schema:about of	named entity 'DENGUE VIRUS' named entity 'patients' named entity 'measured' named entity 'epitopes' named entity 'prediction' named entity 'induce' named entity 'assay' named entity 'epitopes' named entity 'virus-specific' named entity 'ASSAY' named entity 'PRODUCING' named entity 'NUMBERS' named entity 'ELISPOT' named entity 'MONONUCLEAR CELLS' named entity 'MEASURED' named entity 'PEPTIDES' named entity 'STIMULATED' covid:arg/19a5e1a53b1a9d36e3e1956da3c1c184a24979af named entity '396' named entity '310' named entity 'PERCENTAGE' named entity 'SECRETE' named entity '90%' named entity '408' named entity 'SFC' named entity 'CYTOKINE ' named entity 'CD4' named entity 'EPITOPES' named entity 'T-CELL' named entity 'FREQUENCY' named entity 'BASED' named entity 'IDENTIFICATION' named entity 'SOFTWARE' named entity 'ISOLATED' named entity 'PATIENTS' named entity 'PBMC' named entity 'PREDICTION' named entity 'EPITOPES' named entity 'T-CELL' named entity 'CD4' named entity 'COMPUTATIONAL' named entity '0.03' named entity 'RESULT' named entity 'STRAINING' named entity 'STUDY' named entity 'SPECIFIC' named entity 'PREPARED' named entity '141' named entity 'VIRUS TYPE' named entity 'IN VITRO' named entity 'ONLINE' named entity 'FORMING' named entity 'IDENTIFIED' named entity 'USING' named entity 'DERIVED' named entity 'POSITIVE' named entity 'RESPONSE' named entity 'ELISPOT ASSAY' named entity 'TO IDENTIFY' named entity 'INFECTION' named entity 'IFN' named entity 'T CELLS' named entity 'PREDICTION' named entity 'INDUCE' named entity 'CELLS' named entity 'TYPE 1' named entity 'NS3' named entity 'CONVALESCENT' named entity 'DENGUE VIRUS' named entity 'ICS' named entity '2F1' named entity 'SPECIFIC' named entity 'BLOOD' named entity 'T-cell' named entity 'T-cell' named entity 'dengue' named entity 'IFN'

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