About: Simultaneous Detection and Differentiation of Highly Virulent and Classical Chinese-Type Isolation of PRRSV by Real-Time RT-PCR

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About: Simultaneous Detection and Differentiation of Highly Virulent and Classical Chinese-Type Isolation of PRRSV by Real-Time RT-PCR Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Simultaneous Detection and Differentiation of Highly Virulent and Classical Chinese-Type Isolation of PRRSV by Real-Time RT-PCR
Creator	Chen, Yaosheng Gao, Jintao He, Zuyong Liu, Xiaohong Mo, Delin Wang, Liangliang Xiao, Shuqi
Source	PMC
abstract	Porcine reproductive and respiratory syndrome (PRRS) is a leading disease in pig industry worldwide and can result in serious economic losses each year. The PRRS epidemic situation in China has been very complicated since the unprecedented large-scale highly pathogenic PRRS (HP-PRRS) outbreaks in 2006. And now the HP-PRRS virus (HP-PRRSV) and classical North American type PRRSV strains have coexisted in China. Rapid differential detection of the two strains of PRRSV is very important for effective PRRS control. The real-time RT-PCR for simultaneous detection and differentiation of HP-PRRSV and PRRSV by using both SYBR Green and TaqMan probes was developed and validated. Both assays can be used for rapid detection and strain-specific identification of HP-PRRSV and PRRSV. However, the TaqMan probe method had the highest detection rate whereas the conventional RT-PCR was the lowest. The real-time RT-PCR developed based on SYBR Green and TaqMan probe could be used for simultaneous detection and differentiation of HP-PRRSV and PRRSV in China, which will benefit much the PRRS control and research.
has issue date	2014-06-12(xsd:dateTime)
bibo:doi	10.1155/2014/809656
bibo:pmid	25114934
has license	cc-by
sha1sum (hex)	18fb0cf6fba22d9478d4f5774f37f6b6bf09f3b0
schema:url	https://doi.org/10.1155/2014/809656
resource representing a document's title	Simultaneous Detection and Differentiation of Highly Virulent and Classical Chinese-Type Isolation of PRRSV by Real-Time RT-PCR
has PubMed Central identifier	PMC4119655
has PubMed identifier	25114934
schema:publication	J Immunol Res
resource representing a document's body	covid:18fb0cf6fba22d9478d4f5774f37f6b6bf09f3b0#body_text
is schema:about of	named entity 'real-time' named entity 'disease' named entity 'differentiation' named entity 'Rapid' named entity 'conventional' named entity 'Isolation' named entity 'DIFFERENTIATION' named entity 'TYPE' named entity 'CLASSICAL' covid:arg/18fb0cf6fba22d9478d4f5774f37f6b6bf09f3b0 named entity 'VIRULENT' named entity 'PROBES' named entity 'PATHOGENIC' named entity 'VERY IMPORTANT' named entity 'LEADING' named entity 'RT-PCR' named entity 'GREEN ' named entity 'VALIDATED' named entity 'SITUATION' named entity 'TYPE' named entity 'TAQMAN' named entity 'COMPLICATED' named entity 'ECONOMIC' named entity 'PRRS' named entity 'WORLDWIDE' named entity 'BASED' named entity 'SYBR GREEN' named entity 'IDENTIFICATION' named entity 'CHINA' named entity 'RAPID' named entity 'CHINESE' named entity 'PRRSV' named entity 'COULD BE' named entity 'HAVE' named entity 'RATE' named entity 'NORTH AMERICAN' named entity 'USING' named entity 'DISEASE' named entity 'MUCH' named entity 'YEAR' named entity 'REAL-TIME RT-PCR' named entity 'BENEFIT' named entity 'SIMULTANEOUS' named entity 'ISOLATION' named entity 'VIRUS' named entity 'USED' named entity 'INDUSTRY' named entity 'PROBE' named entity 'STRAINS' named entity 'SIMULTANEOUS' named entity 'CONTROL' named entity 'CLASSICAL' named entity 'RESEARCH' named entity 'PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME' named entity 'HIGHLY' named entity 'DIFFERENTIATION' named entity 'RESULT' named entity 'EFFECTIVE' named entity 'DETECTION' named entity 'METHOD' named entity 'REAL-TIME RT-PCR' named entity 'DETECTION' named entity 'IS A' named entity 'DIFFERENTIAL' named entity 'PRRSV' named entity 'PIG' named entity 'CONVENTIONAL' named entity 'OUTBREAKS' named entity 'EPIDEMIC' named entity 'LOWEST' named entity 'HIGHEST' named entity 'HIGHLY' named entity 'effective' named entity 'assays' named entity 'detection'

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