About: Reverse transcription real-time PCR assays for detection and quantification of Borna disease virus in diseased hosts

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Reverse transcription real-time PCR assays for detection and quantification of Borna disease virus in diseased hosts Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Reverse transcription real-time PCR assays for detection and quantification of Borna disease virus in diseased hosts
Creator	Ackermann, M Ehrensperger, F Hilbe, M Puorger, M Schindler, A Vo¨gtlin, A Zlinszky, K
Source	Elsevier; Medline; PMC
abstract	Abstract Borna disease is a severe, immunopathological disorder of the central nervous system caused by the infection with the Borna disease virus (BDV). The detection of BDV in diseased animals, mainly sheep and horses, is achieved by histological, immunohistochemical and serological approaches and/or PCR-based technologies. In the present study, reverse transcription, real-time PCR assays were established for the detection of BDV in the brain tissue from sheep and horses, using loci for the p40 (nucleoprotein) and the p24 (phosphoprotein) genes. The PCRs were equally specific and sensitive, detecting 10 target molecules per reaction and one BDV-infected cell among 106 non-infected cells. In tissues from BDV-diseased sheep and horses, the p24 target was detected at higher abundance than for p40. Therefore, the p24 test is suggested to be of higher value in the diagnostic laboratory. However, both assays should be useful for addressing questions in pathogenesis and for detecting BDV reservoirs in endemic areas.
has issue date	2007-02-28(xsd:dateTime)
bibo:doi	10.1016/j.mcp.2006.08.001
bibo:pmid	17014984
has license	els-covid
sha1sum (hex)	185dd95e212398394200f18a0e93ca1f81c58700
schema:url	https://doi.org/10.1016/j.mcp.2006.08.001
resource representing a document's title	Reverse transcription real-time PCR assays for detection and quantification of Borna disease virus in diseased hosts
has PubMed Central identifier	PMC7127217
has PubMed identifier	17014984
schema:publication	Molecular and Cellular Probes
resource representing a document's body	covid:185dd95e212398394200f18a0e93ca1f81c58700#body_text
is schema:about of	named entity 'REVERSE TRANSCRIPTION' named entity 'RESERVOIRS' named entity 'ADDRESSING' named entity 'DISEASED' named entity 'DETECTION' named entity 'SENSITIVE' named entity 'GENES' named entity 'P40' named entity 'CELLS' named entity 'SHEEP' named entity 'HIGHER' named entity 'CAUSED BY' named entity 'ABUNDANCE' named entity 'BASED' named entity 'BDV' named entity 'IS A' named entity 'SEVERE' named entity 'HORSES' named entity 'TEST' named entity 'SEROLOGICAL' named entity 'USEFUL' named entity 'APPROACHES' named entity 'BORNA DISEASE VIRUS' covid:arg/185dd95e212398394200f18a0e93ca1f81c58700 named entity 'INFECTION' named entity 'PATHOGENESIS' named entity 'HOSTS' named entity 'TECHNOLOGIES' named entity 'DIAGNOSTIC' named entity 'ANIMALS' named entity 'ESTABLISHED' named entity 'REVERSE TRANSCRIPTION' named entity 'LOCI' named entity 'AREAS' named entity 'PCR' named entity 'ENDEMIC' named entity 'HISTOLOGICAL' named entity 'THE BRAIN' named entity 'DISEASED' named entity 'TISSUES' named entity 'PRESENT' named entity 'DETECTED' named entity 'BRAIN TISSUE' named entity 'BORNA DISEASE VIRUS' named entity 'LABORATORY' named entity 'VALUE' named entity 'SPECIFIC' named entity 'MOLECULES' named entity 'DETECTING' named entity 'REAL-TIME PCR ' named entity 'DETECTION' named entity 'QUANTIFICATION' named entity 'TARGET' named entity 'P24' named entity 'REAL-TIME PCR ' named entity 'REACTION' named entity 'STUDY' named entity 'USING' named entity 'INFECTED CELL' named entity 'NON-INFECTED' named entity 'BORNA DISEASE' named entity 'DISORDER OF THE CENTRAL NERVOUS SYSTEM' named entity 'BDV' named entity 'sheep' named entity 'sheep' named entity 'loci' named entity 'immunohistochemical' named entity 'BDV' named entity 'phosphoprotein' named entity 'Borna disease virus' named entity 'Borna' named entity 'cDNA' named entity 'nucleoprotein' named entity 'primers' named entity 'primers'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2025 OpenLink Software