About: Simultaneous detection of severe acute respiratory syndrome, Middle East respiratory syndrome, and related bat coronaviruses by real-time reverse transcription PCR

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About: Simultaneous detection of severe acute respiratory syndrome, Middle East respiratory syndrome, and related bat coronaviruses by real-time reverse transcription PCR Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Simultaneous detection of severe acute respiratory syndrome, Middle East respiratory syndrome, and related bat coronaviruses by real-time reverse transcription PCR
Creator	Kim, Doo-Jin Yoon, Sun-Woo Song, Daesub Na, Woonsung Dae, • Jeong, Gwin Kim, Hye Kim, Ji-Hyung Lee, • Moo-Seung Noh, Ji
source	Medline; PMC
abstract	Since severe acute respiratory syndrome (SARS) and Middle East respiratory syndrome (MERS) coronaviruses (CoVs) share similar characteristics with respect to clinical signs, etiology, and transmission, methods for a rapid and accurate differential diagnosis are important. Therefore, the aim of this study was to develop a duplex real-time reverse transcription (RT)-PCR method for the simultaneous detection of these viruses. Primers and probes that target the conserved spike S2 region of human SARS-CoV, MERS-CoV, and their related bat CoVs were designed. The results of real-time RT-PCR showed specific reactions for each virus with adequate detection limits of 50–100 copies/mL and 5–100 copies/mL using pUC57-SARS-pS2 (a template for SARS-CoV) and pGEM-MERS-S2 (a template for MERS-CoV), respectively. In addition, this real-time RT-PCR system was able to detect the target viruses SARS-like bat CoV and MERS-CoV in bat fecal samples and sputum of MERS patients, respectively. Therefore, this newly developed real-time RT-PCR method is expected to detect not only SARS-CoV and MERS-CoV in humans but also several bat CoVs that are closely related to these viruses in bats.
has issue date	2017-02-20(xsd:dateTime)
bibo:doi	10.1007/s00705-017-3281-9
bibo:pmid	28220326
has license	no-cc
sha1sum (hex)	1723a551b31829262e5c40142e784437b5e3c2d7
schema:url	https://doi.org/10.1007/s00705-017-3281-9
resource representing a document's title	Simultaneous detection of severe acute respiratory syndrome, Middle East respiratory syndrome, and related bat coronaviruses by real-time reverse transcription PCR
has PubMed Central identifier	PMC7086956
has PubMed identifier	28220326
schema:publication	Arch Virol
resource representing a document's body	covid:1723a551b31829262e5c40142e784437b5e3c2d7#body_text
is schema:about of	named entity 'SIMULTANEOUS' named entity 'region' named entity 'Middle East respiratory syndrome' named entity 'bats' named entity 'reverse transcription PCR' named entity 'METHOD' named entity 'VIRUS' named entity 'BAT' named entity 'AIM' named entity 'TEMPLATE' named entity 'PATIENTS' named entity 'IMPORTANT' named entity 'SIMILAR' named entity 'SARS' named entity 'ACCURATE' named entity 'NEWLY' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME' named entity 'CORONAVIRUSES' named entity 'COVS' named entity 'SPUTUM' named entity 'MERS' named entity 'PGEM' named entity 'LIMITS' named entity 'SAMPLES' named entity 'SYSTEM' named entity 'SIMULTANEOUS' named entity 'BUT' named entity '28A' named entity 'TARGET' named entity 'REAL-TIME' named entity 'MIDDLE EAST RESPIRATORY SYNDROME' named entity 'REAL-TIME RT-PCR' named entity 'METHODS' named entity 'RELATED' named entity 'DETECTION' named entity 'COPIES' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME' named entity 'BAT' named entity 'RELATED' named entity 'PRIMERS' named entity 'RESULTS' named entity 'RAPID' named entity 'TO DETECT' named entity '100' named entity 'HUMANS' named entity 'REACTIONS' named entity 'STUDY' named entity 'HUMAN' named entity 'DUPLEX' named entity 'LIKE' named entity 'TRANSMISSION' named entity 'SPIKE' named entity 'SHARE' named entity 'THEIR' named entity 'RESPECT' named entity 'CLINICAL SIGNS' named entity 'REVERSE TRANSCRIPTION PCR' named entity 'EXPECTED' named entity 'USING' named entity 'REAL-TIME' named entity 'ETIOLOGY' named entity 'THESE' named entity 'BATS' named entity 'MIDDLE EAST RESPIRATORY SYNDROME ' named entity 'PROBES' named entity 'REGION' named entity 'MERS-COV' named entity 'VIRUSES' named entity 'CONSERVED' named entity 'DIFFERENTIAL DIAGNOSIS' named entity 'FECAL' named entity 'CHARACTERISTICS'

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