About: Association of Increased Bcl‐2 Expression with Rescue from Tumor Necrosis Factor‐α‐Induced Cell Death in the Oligodendrocyte Cell Line OLN‐93

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About: Association of Increased Bcl‐2 Expression with Rescue from Tumor Necrosis Factor‐α‐Induced Cell Death in the Oligodendrocyte Cell Line OLN‐93 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Association of Increased Bcl‐2 Expression with Rescue from Tumor Necrosis Factor‐α‐Induced Cell Death in the Oligodendrocyte Cell Line OLN‐93
Creator	Brück, ‡wolfgang Burgmaier, Gruscha Kuhlmann, ‡tanja Schönrock, M
Source	Medline; PMC
abstract	Abstract: The present study investigated the effects of flupirtine(Katadolon) on tumor necrosis factor (TNF)‐α‐mediated cell death andBcl‐2 expression in the permanent rat oligodendrocyte cell line OLN‐93 (OLNcells). TNF‐α (500 U/ml) induced apoptosis of OLN cells, which wasconfirmed by DNA fragmentation using an in situ end‐labeling technique andultrastructural analysis. Flupirtine significantly reduced the rate ofspontaneous cell death of OLN cells already at low concentrations;TNF‐α‐mediated apoptosis was suppressed only with higher concentrationsof flupirtine (100 μM). Expression of Bcl‐2 protein and mRNA inOLN cells was detected by immunocytochemistry, western blot, and RT‐PCR.Quantitative analysis of western blots revealed an ∼2.5‐fold up‐regulationof Bcl‐2 protein during TNF‐α treatment. Furthermore, addition of 10 or100 μM flupirtine before incubation with TNF‐α led to anapproximately threefold increase of Bcl‐2 expression. Exposure of OLN cells toflupirtine alone moderately augmented the expression of Bcl‐2 protein. Ourdata demonstrate that flupirtine up‐regulates the expression of Bcl‐2 proteinin OLN cells; this Bcl‐2 induction is associated with a reduced rate ofTNF‐α‐induced cell death.
has issue date	2008-07-29(xsd:dateTime)
bibo:doi	10.1046/j.1471-4159.2000.0752270.x
bibo:pmid	11080178
has license	no-cc
sha1sum (hex)	164fed682f989202e3767cb6dc593e6ded33c9b8
schema:url	https://doi.org/10.1046/j.1471-4159.2000.0752270.x
resource representing a document's title	Association of Increased Bcl‐2 Expression with Rescue from Tumor Necrosis Factor‐α‐Induced Cell Death in the Oligodendrocyte Cell Line OLN‐93
has PubMed Central identifier	PMC7166700
has PubMed identifier	11080178
schema:publication	J Neurochem
resource representing a document's body	covid:164fed682f989202e3767cb6dc593e6ded33c9b8#body_text
is schema:about of	named entity 'protein' named entity 'reduced' named entity 'higher' named entity 'reduced' named entity 'OLIGODENDROCYTE' named entity 'cells' named entity 'concentrations' named entity 'data' named entity 'tumor necrosis factor' named entity 'protein' named entity 'concentrations' named entity 'OLN' named entity 'incubation' named entity 'OLN' named entity 'Bcl-2' named entity 'mRNA' named entity 'Bcl-2' named entity 'protein' named entity 'TNF' named entity 'tumor necrosis factor' named entity 'flupirtine' named entity 'Bcl-2' named entity 'RT-PCR' named entity 'cell death' named entity 'TNF' named entity 'ultrastructural' named entity 'protein' named entity 'Bcl-2' named entity 'Cell Death' named entity 'Bcl-2' named entity 'protein' named entity 'cell death' named entity 'oligodendrocyte' named entity 'oligodendrocyte' named entity 'TNF' named entity 'infection' named entity 'Hilden' named entity 'Student's t test' named entity 'flupirtine' named entity 'TUNEL' named entity 'flupirtine' named entity 'electron microscope' named entity 'mRNA' named entity 'TNF' named entity 'oligodendrocytes' named entity 'ischemic damage' named entity 'Fas' named entity 'Berlin' named entity 'interferon' named entity 'flupirtine' named entity 'Oligodendrocytes' named entity 'oligodendrocytes' named entity 'analgesic' named entity 'Wiesbaden' named entity 'Bcl-2' named entity 'Cell death' named entity 'protein' named entity 'Dulbecco's modified Eagle's medium' named entity 'protein' named entity 'flupirtine' named entity 'Bcl-2' named entity 'electron microscopy' named entity 'apoptosis' named entity 'oligodendrocytes' named entity 'oligodendrocyte' named entity '37°C' named entity 'Genzyme' named entity 'apoptotic' named entity 'oligodendrocyte' named entity 'Araldite' named entity 'cDNA' named entity 'propylene oxide' named entity 'demyelination' named entity 'phosphate' named entity 'Asta Medica' named entity 'infection' named entity 'Bcl-2' named entity 'fetal calf serum'

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