About: Crystallization and preliminary X-ray diffraction analysis of Nsp15 from SARS coronavirus

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About: Crystallization and preliminary X-ray diffraction analysis of Nsp15 from SARS coronavirus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Crystallization and preliminary X-ray diffraction analysis of Nsp15 from SARS coronavirus
Creator	Canard, Bruno Coutard, Bruno Debarnot, Claire Cambillau, Christian Campanacci, Valérie Egloff, Marie-Pierre Brémond, Nicolas Dalle, Karen Grisel, Sacha Lantez, Violaine Lichière, Julie Tocque, Fabienne Ricagno, Stéfano
topic	covid:15e51670da2c8abe7493cd04575498a30dba4ae6#this
Source	Medline; PMC
abstract	The non-structural protein Nsp15 from the aetiological agent of SARS (severe acute respiratory syndrome) has recently been characterized as a uridine-specific endoribonuclease. This enzyme plays an essential role in viral replication and transcription since a mutation in the related H229E human coronavirus nsp15 gene can abolish viral RNA synthesis. SARS full-length Nsp15 (346 amino acids) has been cloned and expressed in Escherichia coli with an N-terminal hexahistidine tag and has been purified to homogeneity. The protein was subsequently crystallized using PEG 8000 or 10 000 as precipitants. Small cubic crystals of the apoenzyme were obtained from 100 nl nanodrops. They belong to space group P4(1)32 or P4(3)32, with unit-cell parameters a = b = c = 166.8 Å. Diffraction data were collected to a maximum resolution of 2.7 Å.
has issue date	2006-03-25(xsd:dateTime)
bibo:doi	10.1107/s1744309106009407
bibo:pmid	16582498
has license	bronze-oa
sha1sum (hex)	15e51670da2c8abe7493cd04575498a30dba4ae6
schema:url	https://doi.org/10.1107/s1744309106009407
resource representing a document's title	Crystallization and preliminary X-ray diffraction analysis of Nsp15 from SARS coronavirus
has PubMed Central identifier	PMC2222560
has PubMed identifier	16582498
schema:publication	Acta Crystallographica Section F Structural Biology and Crystallization Communications
resource representing a document's body	covid:15e51670da2c8abe7493cd04575498a30dba4ae6#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/15e51670da2c8abe7493cd04575498a30dba4ae6
is schema:about of	named entity 'viral replication' named entity 'protein' named entity 'Crystallization' named entity 'USING' named entity 'CELL' named entity 'APOENZYME' named entity 'AGENT' named entity 'X-RAY DIFFRACTION ANALYSIS' named entity 'COLLECTED' named entity 'RESPIRATORY' named entity 'UNIT' named entity 'TRANSCRIPTION' named entity '166' named entity 'VIRAL REPLICATION' named entity '28346' named entity 'EXPRESSED' named entity 'CRYSTALLIZED' named entity 'RECENTLY' named entity 'CHARACTERIZED' named entity 'SMALL' named entity 'CRYSTALLIZATION' named entity 'PEG 8000' named entity 'STRUCTURAL BIOLOGY' named entity 'ESCHERICHIA COLI' named entity 'ACUTE' named entity 'MAXIMUM' named entity 'SYNDROME' named entity 'ROLE' named entity 'VIRAL RNA' named entity 'DIFFRACTION DATA' named entity 'PROTEIN ' named entity 'HUMAN CORONAVIRUS' named entity 'SPACE GROUP' named entity 'STRUCTURAL PROTEIN' named entity 'PARAMETERS' named entity 'NON-' named entity 'TAG' named entity 'SARS' named entity '100' named entity 'OBTAINED' named entity 'ESSENTIAL' named entity 'GENE' named entity 'AMINO ACIDS' named entity 'ENZYME' named entity 'LENGTH' named entity 'HOMOGENEITY' named entity 'ENDORIBONUCLEASE' named entity 'RELATED' named entity 'HEXAHISTIDINE' named entity 'RESOLUTION' named entity '2.7' named entity 'N-TERMINAL ' named entity 'RNA SYNTHESIS' named entity 'CUBIC' named entity 'COMMUNICATIONS' named entity 'PRELIMINARY' named entity 'MUTATION' named entity 'PLAYS' named entity 'PURIFIED' named entity 'SARS CORONAVIRUS' named entity 'CRYSTALS' named entity 'CLONED' named entity 'N-terminal' named entity 'cubic crystals' named entity 'expressed' named entity 'transcription' named entity 'protein'

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