About: Optimizing the refolding conditions of self-assembling polypeptide nanoparticles that serve as repetitive antigen display systems

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About: Optimizing the refolding conditions of self-assembling polypeptide nanoparticles that serve as repetitive antigen display systems Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Optimizing the refolding conditions of self-assembling polypeptide nanoparticles that serve as repetitive antigen display systems
Creator	Burkhard, Peter Ringler, Philippe Yang, Yongkun Müller, Shirley
Source	Elsevier; Medline; PMC
abstract	Nanoparticles show great promise as potent vaccine candidates since they are readily taken up by the antigen presenting cells of the immune system. The particle size and the density of the B cell epitopes on the surface of the particles greatly influences the strength of the humoral immune response. We have developed a novel type of nanoparticle composed of peptide building blocks (Raman et al., 2006) and have used such particles to design vaccines against malaria and SARS (Kaba et al., 2009, Pimentel et al., 2009). Here we investigate the biophysical properties and the refolding conditions of a prototype of these self-assembling polypeptide nanoparticles (SAPNs). SAPNs are formed from a peptide containing a pentameric and a trimeric coiled-coil domain. At near physiological conditions the peptide self-assembles into about 27 nm, roughly spherical SAPNs. The average size of the SAPNs increases with the salt concentration. The optimal pH for their formation is between 7.5 and 8.5, while aggregation occurs at lower and higher values. A glycerol concentration of about 5% v/v is required for the formation of SAPNs with regular spherical shapes. These studies will help to optimize the immunological properties of SAPNs.
has issue date	2011-11-17(xsd:dateTime)
bibo:doi	10.1016/j.jsb.2011.11.011
bibo:pmid	22115997
has license	no-cc
sha1sum (hex)	15c9fa3a8688b37209548ba87ed5e94fe0acdc86
schema:url	https://doi.org/10.1016/j.jsb.2011.11.011
resource representing a document's title	Optimizing the refolding conditions of self-assembling polypeptide nanoparticles that serve as repetitive antigen display systems
has PubMed Central identifier	PMC7118850
has PubMed identifier	22115997
schema:publication	J Struct Biol
resource representing a document's body	covid:15c9fa3a8688b37209548ba87ed5e94fe0acdc86#body_text
is schema:about of	named entity 'IMMUNE SYSTEM' named entity 'VALUES' named entity 'LOWER' named entity 'AGGREGATION' named entity '8.5' named entity 'DENSITY' named entity 'SARS' named entity 'STRENGTH' named entity 'POLYPEPTIDE' named entity 'SHAPES' named entity 'HUMORAL IMMUNE RESPONSE' named entity 'HELP' named entity 'OPTIMAL' named entity 'CONCENTRATION OF' named entity 'GREATLY' named entity 'VACCINES' named entity 'pentameric' named entity 'vaccine' named entity 'Raman' named entity 'optimize' named entity 'Nanoparticles' named entity 'average' named entity 'serve' named entity 'conditions' named entity 'SERVE' named entity 'CONDITIONS' named entity 'GLYCEROL' named entity 'NANOPARTICLE' named entity 'REQUIRED' named entity 'COMPOSED OF' named entity 'PROPERTIES' named entity 'REFOLDING' named entity 'NEAR' named entity 'CONCENTRATION' named entity 'PHYSIOLOGICAL' named entity 'NANOPARTICLES' named entity 'CONTAINING' named entity 'PROMISE' named entity 'THESE' named entity 'ABOUT' named entity 'FORMATION' named entity 'DESIGN' named entity 'FORMED' named entity 'SURFACE' named entity 'HAVE' named entity 'EPITOPES' named entity 'TAKEN' named entity 'PEPTIDE' named entity 'USED' named entity 'POLYPEPTIDE' named entity 'REFOLDING' named entity 'DISPLAY' named entity 'ANTIGEN' named entity 'SYSTEMS' named entity 'VACCINE' named entity 'SPHERICAL' named entity 'TYPE' named entity 'CONDITIONS' named entity 'OPTIMIZE' named entity 'B CELL' named entity 'REGULAR' named entity 'BLOCKS' named entity 'STUDIES' named entity 'NANOPARTICLES' named entity 'REPETITIVE' named entity 'GREAT' named entity 'PROTOTYPE' named entity 'INVESTIGATE' named entity 'MALARIA' named entity 'COILED-COIL DOMAIN' named entity 'NOVEL' named entity 'AVERAGE' named entity 'ANTIGEN PRESENTING CELLS' named entity 'SIZE' named entity '7.5' named entity 'BUILDING' named entity 'INCREASES' named entity 'BIOPHYSICAL'

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