About: miR-21a-5p Contributes to Porcine Hemagglutinating Encephalomyelitis Virus Proliferation via Targeting CASK-Interactive Protein1 In vivo and vitro

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: miR-21a-5p Contributes to Porcine Hemagglutinating Encephalomyelitis Virus Proliferation via Targeting CASK-Interactive Protein1 In vivo and vitro Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	miR-21a-5p Contributes to Porcine Hemagglutinating Encephalomyelitis Virus Proliferation via Targeting CASK-Interactive Protein1 In vivo and vitro
Creator	Nal, Béatrice Adachi, Akio Fuller, Frederick
Source	Medline; PMC
abstract	Porcine hemagglutinating encephalomyelitis virus (PHEV) is a highly neurovirulent coronavirus that can cause nervous symptoms in piglets with muscle tremors, hind limb paralysis, and nystagmus. Whether some factors affect virus replication and proliferation had not been fully understood in the course of nerve damage caused by PHEV infection. In recent years, some reports suggested that miRNA might play a key regulatory role in viral infection. In this study, we found the miR-21a-5p is notably up-regulated in the brains of mice and N2a cells infected with PHEV, and it down-regulated the expression of CASK-interactive protein1 (Caskin1) by directly targeting the 3′-UTR of Caskin1 using a Dual-Luciferase reporter assay. The over-expression of miR-21a-5p or Caskin1 knockdown in the host significantly contributes to PHEV proliferation. Conversely, the silencing of miR-21a-5p by miR-21a-5p inhibitors suppressed the virus proliferation. Taken together, our results indicate that Caskin1 is the direct target gene of miR-21a-5p, and it is advantageous to virus proliferation by down-regulating Caskin1. These findings may help in the development of strategies for therapeutic applications.
has issue date	2017-03-01(xsd:dateTime)
bibo:doi	10.3389/fmicb.2017.00304
bibo:pmid	28298907
has license	cc-by
sha1sum (hex)	14723bfc42908c3f8f522692be41062a7cda62d4
schema:url	https://doi.org/10.3389/fmicb.2017.00304
resource representing a document's title	miR-21a-5p Contributes to Porcine Hemagglutinating Encephalomyelitis Virus Proliferation via Targeting CASK-Interactive Protein1 In vivo and vitro
has PubMed Central identifier	PMC5331037
has PubMed identifier	28298907
schema:publication	Front Microbiol
resource representing a document's body	covid:14723bfc42908c3f8f522692be41062a7cda62d4#body_text
is schema:about of	named entity 'neurons' named entity 'miR-21' named entity 'cerebral cortex' named entity 'UTR' named entity 'qRT-PCR' named entity 'brain tissue' named entity 'miR' named entity 'PBS' named entity 'apoptosis' named entity 'regulate the expression' named entity 'brain tissues' named entity 'miRNA' named entity 'regulate the expression' named entity 'Trizol' named entity 'PHEV' named entity 'PHEV' named entity 'BALB/c' named entity 'miR' named entity 'microRNA' named entity 'fetal bovine serum' named entity 'PDCD4' named entity 'SYBR Green' named entity 'World Health Organization' named entity 'miR' named entity 'brain tissues' named entity 'endogenous' named entity 'polyacrylamide gels' named entity 'infection' named entity 'mice' named entity 'up-regulation' named entity 'Chicago' named entity 'neurotoxicity' named entity 'PHEV' named entity 'brain tissue' named entity 'untranslated region' named entity 'infection' named entity 'Animal Welfare' named entity 'virus infection' named entity 'neurovirulent' named entity 'UTR' named entity 'encephalomyelitis' named entity 'mice' named entity 'PHEV' named entity 'UTR' named entity 'PCR' named entity 'PHEV' named entity 'mouse brain' named entity 'RNA' named entity 'miR' named entity 'Triton X-100' named entity 'RNA' named entity 'negative control' named entity 'miR' named entity 'plasmid' named entity 'host cell' named entity 'proteases' named entity 'miR' named entity 'miRNA' named entity 'miR' named entity 'virus infection' named entity 'Histogram' named entity 'miR-21' named entity 'culture plates' named entity 'NF-κB' named entity 'PBS' named entity 'miR-21' named entity 'mice' named entity 'vomiting' named entity 'miRNA' named entity '1:500' named entity 'PHEV' named entity 'biological functions' named entity 'viruses' named entity 'herpes simplex virus 1' named entity 'PHEV' named entity 'Porcine' named entity 'expression profile' named entity 'binding site' named entity 'polyclonal antibody'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2025 OpenLink Software