About: The effects of Nigella sativa (Ns), Anthemis hyalina (Ah) and Citrus sinensis (Cs) extracts on the replication of coronavirus and the expression of TRP genes family

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About: The effects of Nigella sativa (Ns), Anthemis hyalina (Ah) and Citrus sinensis (Cs) extracts on the replication of coronavirus and the expression of TRP genes family Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	The effects of Nigella sativa (Ns), Anthemis hyalina (Ah) and Citrus sinensis (Cs) extracts on the replication of coronavirus and the expression of TRP genes family
Creator	Ulasli, Mustafa Arslan, Ahmet Bayraktar, Recep Cakmak, Ali Ecir, • Gurses, Serdar Igci, Ziya Mehri, Oztuzcu Onder, • Serdar, Yumrutas Yusuf, Igci
topic	covid:145f37b63789de6209b2d69b56ea22f8755d76f1#this
source	Medline; PMC
abstract	Extracts of Anthemis hyalina (Ah), Nigella sativa (Ns) and peels of Citrus sinensis (Cs) have been used as folk medicine to fight antimicrobial diseases. To evaluate the effect of extracts of Ah, Ns and Cs on the replication of coronavirus (CoV) and on the expression of TRP genes during coronavirus infection, HeLa-CEACAM1a (HeLa-epithelial carcinoembryonic antigen-related cell adhesion molecule 1a) cells were inoculated with MHV-A59 (mouse hepatitis virus–A59) at moi of 30. 1/50 dilution of the extracts was found to be the safe active dose. ELISA kits were used to detect the human IL-8 levels. Total RNA was isolated from the infected cells and cDNA was synthesized. Fluidigm Dynamic Array nanofluidic chip 96.96 was used to analyze the mRNA expression of 21 TRP genes and two control genes. Data was analyzed using the BioMark digital array software. Determinations of relative gene expression values were carried out by using the 2(−∆∆Ct) method (normalized threshold cycle (Ct) value of sample minus normalized Ct value of control). TCID(50)/ml (tissue culture infectious dose that will produce cytopathic effect in 50 % of the inoculated tissue culture cells) was found for treatments to determine the viral loads. The inflammatory cytokine IL-8 level was found to increase for both 24 and 48 h time points following Ns extract treatment. TRPA1, TRPC4, TRPM6, TRPM7, TRPM8 and TRPV4 were the genes which expression levels changed significantly after Ah, Ns or Cs extract treatments. The virus load decreased when any of the Ah, Ns or Cs extracts was added to the CoV infected cells with Ah extract treatment leading to undetectable virus load for both 6 and 8hpi. Although all the extract treatments had an effect on IL-8 secretion, TRP gene expression and virus load after CoV infection, it was the Ah extract treatment that showed the biggest difference in virus load. Therefore Ah extract is the best candidate in our hands that contains potential treatment molecule(s). ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11033-014-3019-7) contains supplementary material, which is available to authorized users.
has issue date	2014-01-12(xsd:dateTime)
bibo:doi	10.1007/s11033-014-3019-7
bibo:pmid	24413991
has license	no-cc
sha1sum (hex)	145f37b63789de6209b2d69b56ea22f8755d76f1
schema:url	https://doi.org/10.1007/s11033-014-3019-7
resource representing a document's title	The effects of Nigella sativa (Ns), Anthemis hyalina (Ah) and Citrus sinensis (Cs) extracts on the replication of coronavirus and the expression of TRP genes family
has PubMed Central identifier	PMC3933739
has PubMed identifier	24413991
schema:publication	Mol Biol Rep
resource representing a document's body	covid:145f37b63789de6209b2d69b56ea22f8755d76f1#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/145f37b63789de6209b2d69b56ea22f8755d76f1
is schema:about of	named entity 'IL-8' named entity 'Citrus sinensis' covid:arg/145f37b63789de6209b2d69b56ea22f8755d76f1 named entity 'dilution' named entity 'diseases' named entity 'TRP' named entity 'expression' named entity 'antimicrobial' named entity 'Anthemis' named entity 'coronavirus infection' named entity 'folk medicine' named entity 'Nigella sativa' named entity 'infection' named entity 'Fluidigm' named entity 'TRPM6' named entity 'calcium' named entity 'IL-8' named entity 'infection' named entity 'CoV' named entity 'enveloped viruses' named entity 'calcium' named entity 'nucleocapsid' named entity 'non-toxic' named entity 'San Francisco, CA' named entity 'HeLa' named entity 'virus replication' named entity 'TRPV4' named entity 'supernatants' named entity 'expression levels' named entity 'Anthemis' named entity 'penicillin' named entity 'intracellular' named entity 'toxic effect' named entity 'RT PCR' named entity 'virus' named entity 'IL-8' named entity '1/50' named entity 'insecticidal' named entity 'A59' named entity 'GAPDH' named entity 'transient receptor potential' named entity 'Intracellular' named entity 'sweet orange' named entity 'fetal calf serum' named entity 'cytotoxic' named entity 'A59' named entity 'extracellular' named entity 'systemic diseases' named entity '1/50' named entity 'Chamomile' named entity 'HeLa' named entity 'TRPV4' named entity 'gene expression' named entity 'HeLa' named entity 'hyalina' named entity 'up-regulation' named entity 'toxic effects' named entity 'gene' named entity 'Chamomile' named entity 'mRNA expression' named entity 'replication cycle' named entity 'genus' named entity 'radiation source' named entity 'carvacrol' named entity 'viruses' named entity 'CoV' named entity 'approved drugs' named entity 'ethanol' named entity 'Rochester, NY'

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