About: Intracellular localization of the SARS coronavirus protein 9b: Evidence of active export from the nucleus

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Intracellular localization of the SARS coronavirus protein 9b: Evidence of active export from the nucleus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Intracellular localization of the SARS coronavirus protein 9b: Evidence of active export from the nucleus
Creator	Zakhartchouk, Alexander Petric, Martin Viswanathan, Sathiyanarayanan Babiuk, Lorne Lobanov, Vladislav Moshynskyy, Igor Vasilenko, Natalia
Source	Elsevier; Medline; PMC
abstract	Abstract Open reading frame 9b (ORF 9b) encodes a 98 amino acid group-specific protein of severe acute respiratory syndrome (SARS) coronavirus (CoV). It has no homology with known proteins and its function in SARS CoV replication has not been determined. The N-terminal part of the 9b protein was used to raise polyclonal antibodies in rabbits, and these antibodies could detect 9b protein in infected cells. We analyzed the sub-cellular localization of recombinant 9b protein using fluorescence microscopy of live transfected cells and indirect immunofluorescence of transfected fixed cells. Our findings indicate that the 9b protein is exported outside of a cell nucleus and localizes to the endoplasmic reticulum. Our data also suggest that the 46-LRLGSQLSL-54 amino acid sequence of 9b functions as a nuclear export signal (NES).
has issue date	2007-07-31(xsd:dateTime)
bibo:doi	10.1016/j.virusres.2007.03.011
bibo:pmid	17448558
has license	els-covid
sha1sum (hex)	14173ffbb5c50c31fa0280660622bee416587339
schema:url	https://doi.org/10.1016/j.virusres.2007.03.011
resource representing a document's title	Intracellular localization of the SARS coronavirus protein 9b: Evidence of active export from the nucleus
has PubMed Central identifier	PMC7114319
has PubMed identifier	17448558
schema:publication	Virus Research
resource representing a document's body	covid:14173ffbb5c50c31fa0280660622bee416587339#body_text
is schema:about of	named entity 'Our' named entity 'protein' named entity 'homology' named entity 'infected' named entity 'protein' named entity 'fixed' named entity 'Intracellular' covid:arg/14173ffbb5c50c31fa0280660622bee416587339 named entity 'protein' named entity 'replication' named entity 'cloned' named entity 'endoplasmic reticulum' named entity 'EcoRI' named entity 'serum' named entity 'ceramide' named entity 'antibodies' named entity 'protein' named entity 'transfected' named entity 'fusion protein' named entity 'EcoRI' named entity 'codons' named entity 'cell nuclei' named entity 'ORF' named entity 'fluorescence' named entity 'gene' named entity 'cellular localization' named entity 'lipid' named entity 'gene' named entity 'transfected' named entity 'SARS' named entity 'homology' named entity 'cross-reactivity' named entity '2a-c' named entity 'gel electrophoresis' named entity 'ATCC' named entity 'DNA sequencing' named entity 'SARS-CoV' named entity 'bacterial cultures' named entity 'cloned' named entity 'antibodies' named entity 'serum' named entity 'gene' named entity 'Sambrook' named entity 'fluorescence' named entity 'cytoplasmic' named entity 'electrophoretic mobility' named entity 'transfected' named entity 'BD Biosciences' named entity 'nuclear envelope' named entity 'Vero E6' named entity 'intracellular' named entity 'Golgi complex' named entity 'live cells' named entity 'fluorescence' named entity 'fluorescence' named entity 'nucleotides' named entity 'MOI' named entity 'GST' named entity 'endoplasmic reticulum' named entity 'transfected' named entity 'transfection' named entity 'human immunodeficiency virus' named entity 'Hong Kong' named entity 'ORF' named entity 'nuclear export sequence' named entity 'Western blot' named entity 'transfected' named entity 'Adenovirus' named entity 'nuclear export signal' named entity 'acid' named entity 'proteins' named entity 'localizes' named entity 'SARS CoV' named entity 'NES' named entity 'sub-cellular localization'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software