About: New oligonucleotide microarray for rapid diagnosis of avian viral diseases

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About: New oligonucleotide microarray for rapid diagnosis of avian viral diseases Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	New oligonucleotide microarray for rapid diagnosis of avian viral diseases
Creator	Sansyzbay, Abylay Burashev, Yerbol Chervyakova, Olga Kozhabergenov, Nurlan Orynbayev, Mukhit Rametov, Nurkuisa Sandybayev, Nurlan Shorayeva, Kamshat Strochkov, Vitaliy Sultankulova, Kulyaisan
Source	Medline; PMC
abstract	BACKGROUND: We developed a new oligonucleotide microarray comprising 16 identical subarrays for simultaneous rapid detection of avian viruses: avian influenza virus (AIV), Newcastle disease virus (NDV), infection bronchitis virus (IBV), and infectious bursal disease virus (IBDV) in single- and mixed-virus infections. The objective of the study was to develop an oligonucleotide microarray for rapid diagnosis of avian diseases that would be used in the course of mass analysis for routine epidemiological surveillance owing to its ability to test one specimen for several infections. METHODS AND RESULTS: The paper describes the technique for rapid and simultaneous diagnosis of avian diseases such as avian influenza, Newcastle disease, infectious bronchitis and infectious bursal disease with use of oligonucleotide microarray, conditions for hybridization of fluorescent-labelled viral cDNA on the microarray and its specificity tested with use of AIV, NDV, IBV, IBDV strains as well as biomaterials from poultry. Sensitivity and specificity of the developed microarray was evaluated with use of 122 specimens of biological material: 44 cloacal swabs from sick birds and 78 tissue specimens from dead wild and domestic birds, as well as with use of 15 AIV, NDV, IBV and IBDV strains, different in their origin, epidemiological and biological characteristics (RIBSP Microbial Collection). This microarray demonstrates high diagnostic sensitivity (99.16% within 95% CI limits 97.36–100%) and specificity (100%). Specificity of the developed technique was confirmed by direct sequencing of NP and M (AIV), VP2 (IBDV), S1 (IBV), NP (NDV) gene fragments. CONCLUSION: Diagnostic effectiveness of the developed DNA microarray is 99.18% and therefore it can be used in mass survey for specific detection of AIV, NDV, IBV and IBDV circulating in the region in the course of epidemiological surveillance. Rather simple method for rapid diagnosis of avian viral diseases that several times shortens duration of assay versus classical diagnostic methods is proposed.
has issue date	2017-04-05(xsd:dateTime)
bibo:doi	10.1186/s12985-017-0738-0
bibo:pmid	28381285
has license	cc-by
sha1sum (hex)	13b8c068799a93b4441116bb6cabae222d126d32
schema:url	https://doi.org/10.1186/s12985-017-0738-0
resource representing a document's title	New oligonucleotide microarray for rapid diagnosis of avian viral diseases
has PubMed Central identifier	PMC5382490
has PubMed identifier	28381285
schema:publication	Virol J
resource representing a document's body	covid:13b8c068799a93b4441116bb6cabae222d126d32#body_text
is schema:about of	named entity 'TO TEST' named entity 'SIMULTANEOUS' named entity 'BRONCHITIS' named entity 'AVIAN INFLUENZA VIRUS' named entity 'RAPID DIAGNOSIS' named entity 'INFECTIOUS BURSAL DISEASE VIRUS' named entity 'ROUTINE' named entity 'SURVEILLANCE' named entity 'DETECTION' named entity 'SINGLE' named entity 'SPECIMEN' named entity 'VIRUSES' named entity 'VIRAL DISEASES' named entity 'NDV' named entity 'microarray' named entity 'viral diseases' named entity 'VIRUS' named entity 'NEWCASTLE DISEASE VIRUS' named entity 'IDENTICAL' named entity 'OBJECTIVE' named entity 'INFECTION' named entity 'COURSE' named entity 'ITS' named entity 'INFECTIONS' named entity 'AVIAN DISEASES' named entity 'NEW' named entity 'NEW' named entity 'AVIAN' named entity 'RAPID DIAGNOSIS' named entity 'STUDY' named entity 'USED' named entity 'RAPID' named entity 'OLIGONUCLEOTIDE MICROARRAY' named entity 'MIXED' named entity 'MASS ANALYSIS' named entity 'EPIDEMIOLOGICAL' named entity 'OLIGONUCLEOTIDE MICROARRAY' named entity 'AVIAN' covid:arg/13b8c068799a93b4441116bb6cabae222d126d32 named entity 'detection' named entity 'Newcastle disease virus' named entity 'developed' named entity 'infection' named entity 'IBDV' named entity 'NDV' named entity 'epidemiological surveillance' named entity 'avian influenza virus' named entity 'IBV' named entity 'California' named entity 'real-time RT-PCR' named entity 'IBDV' named entity 'PCR-products' named entity 'IBV' named entity 'IBDV' named entity 'IBV' named entity 'virus isolation' named entity 'France' named entity 'Germany' named entity 'virus isolation' named entity 'infection' named entity 'Koktal' named entity 'real-time RT-PCR' named entity 'NCBI' named entity 'Sequence Database' named entity 'veterinarians' named entity 'DNA microarray' named entity 'NDV' named entity 'NDV' named entity 'causative agent' named entity 'incubation' named entity 'industrial farms' named entity 'Korday district'

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