About: Phenotypic characteristics of human type II alveolar epithelial cells suitable for antigen presentation to T lymphocytes

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About: Phenotypic characteristics of human type II alveolar epithelial cells suitable for antigen presentation to T lymphocytes Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Phenotypic characteristics of human type II alveolar epithelial cells suitable for antigen presentation to T lymphocytes
Creator	Cappello, Mattéo Corbière, Véronique Dirix, Violette Mascart, Françoise Norrenberg, Sarah Remmelink, Myriam
Source	Medline; PMC
abstract	BACKGROUND: Type II alveolar epithelial cells (AECII) are well known for their role in the innate immune system. More recently, it was proposed that they could play a role in the antigen presentation to T lymphocytes but contradictory results have been published both concerning their surface expressed molecules and the T lymphocyte responses in mixed lymphocyte cultures. The use of either AECII cell line or fresh cells could explain the observed discrepancies. Thus, this study aimed at defining the most relevant model of accessory antigen presenting cells by carefully comparing the two models for their expression of surface molecules necessary for efficient antigen presentation. METHODS: We have compared by flow cytometry the surface expression of the major markers involved in the immunological synapse on the A549 cell line, the most popular model of type II alveolar epithelial cells, and freshly isolated cells. HLA-DR, CD80, CD86, ICOS-L, CD54, CD58 surface expression were studied in resting conditions as well as after IFN-γ/TNF-α treatment, two inflammatory cytokines, known to modulate some of these markers. RESULTS: The major difference found between the two cells types was the very low surface expression of HLA-DR on the A549 cell line compared to its constitutive expression on freshly isolated AECII. The surface expression of co-stimulatory molecules from the B7 family was very low for the CD86 (B7-2) and ICOS-L (B7-H2) and absent for CD80 (B7-1) on both freshly isolated cells and A549 cell line. Neither IFN-γ nor TNF-α could increase the expression of these classical co-stimulatory molecules. However CD54 (ICAM-1) and CD58 (LFA-3) adhesion molecules, known to be implicated in B7 independent co-stimulatory signals, were well expressed on the two cell types. CONCLUSIONS: Constitutive expression of MHC class I and II molecules as well as alternative co-stimulatory molecules by freshly isolated AECII render these cells a good model to study antigen presentation.
has issue date	2011-01-24(xsd:dateTime)
bibo:doi	10.1186/1465-9921-12-15
bibo:pmid	21261956
has license	cc-by
sha1sum (hex)	12fe4cca998a403e153ae872595eab2676c77d2f
schema:url	https://doi.org/10.1186/1465-9921-12-15
resource representing a document's title	Phenotypic characteristics of human type II alveolar epithelial cells suitable for antigen presentation to T lymphocytes
has PubMed Central identifier	PMC3033824
has PubMed identifier	21261956
schema:publication	Respir Res
resource representing a document's body	covid:12fe4cca998a403e153ae872595eab2676c77d2f#body_text
is schema:about of	named entity 'INNATE IMMUNE SYSTEM' named entity 'RELEVANT' named entity 'EFFICIENT' named entity 'MOLECULES' named entity 'HAVE' named entity 'ANTIGEN PRESENTING CELLS' named entity 'PROPOSED' named entity 'STUDY' named entity 'MODEL OF' named entity 'CELLS' named entity 'ROLE' named entity 'RECENTLY' named entity 'USE OF' named entity 'ALVEOLAR EPITHELIAL CELLS' named entity 'KNOWN' named entity 'T LYMPHOCYTE' named entity 'comparing' named entity 'published' named entity 'expression' named entity 'antigen presentation' named entity 'TYPE II' named entity 'CELL LINE' named entity 'THEIR' named entity 'RESPONSES' named entity 'RESULTS' named entity 'ANTIGEN PRESENTATION' named entity 'NECESSARY' named entity 'DISCREPANCIES' named entity 'FRESH' named entity 'HUMAN' named entity 'SUITABLE' named entity 'ANTIGEN PRESENTATION' named entity 'CHARACTERISTICS' named entity 'T LYMPHOCYTES' named entity 'EITHER' named entity 'BACKGROUND' named entity 'COMPARING' named entity 'TYPE II' named entity 'EXPRESSED' named entity 'OBSERVED' named entity 'PUBLISHED' named entity 'ACCESSORY' named entity 'ALVEOLAR EPITHELIAL CELLS' named entity 'BUT' named entity 'DEFINING' named entity 'PLAY' named entity 'LYMPHOCYTE' named entity 'T LYMPHOCYTES' named entity 'CULTURES' named entity 'EXPRESSION' named entity 'SURFACE' named entity 'MIXED' named entity 'MODELS' covid:arg/12fe4cca998a403e153ae872595eab2676c77d2f named entity 'molecules' named entity 'Type II' named entity 'models' named entity 'More' named entity 'lymphocyte' named entity 'antigen presentation' named entity 'cell line' named entity 'alveolar epithelial cells' named entity 'ICOS' named entity 'staining' named entity 'permeabilization' named entity 'antigen-presenting cells' named entity 'Mann-Whitney' named entity 'A549' named entity 'phenotype' named entity 'lymphocytes' named entity 'CD4 +' named entity 'IgG2a' named entity 'CD8 +' named entity 'memory T lymphocytes' named entity 'TNF-α' named entity 'co-stimulatory molecules'

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