About: Fast SARS-CoV-2 detection by RT-qPCR in preheated nasopharyngeal swab samples

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About: Fast SARS-CoV-2 detection by RT-qPCR in preheated nasopharyngeal swab samples Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Fast SARS-CoV-2 detection by RT-qPCR in preheated nasopharyngeal swab samples
Creator	Flores, Carlos Valenzuela-Fernández, Agustín González-Montelongo, Rafaela Alcoba-Florez, Julia Ciuffreda, Laura García-Martínez De Artola, Diego Gil-Campesino, Helena Beltrán-Tacoronte, Concepción Candelaria, M Espinel-Guerra, Guacimara González-González, Victoria Gómez-Ruíz, Esther Manrique-Izquierdo, Teodora Medina-Coello, Chaxiraxi Rivera-Ruiz, Rocio Sonia Batista-Torres, $ Trujillo-Medina, Iru
Source	MedRxiv
abstract	The current reference for COVID-19 diagnosis is based on the detection of SARS-CoV-2 on RNA extracts using one-step retrotranscription and quantitative PCR (RT-qPCR). Based on the urgent need for high-throughput COVID-19 screening, we tested the performance of three alternative, simple and affordable protocols to rapidly detect SARS-CoV-2, overcoming the long and tedious RNA extraction step. Although with an average increase of 6.1 ( ±1.6) cycles compared to standard tests with RNA extracts, we show that RT-qPCR yielded consistent results in nasopharyngeal swab samples that were subject to a direct 70°C incubation for 10 min. Our findings provide viable options to overcome any supply chain issue and help to increase the throughput of diagnostic tests by using any qPCR device, thereby complementing standard COVID-19 testing.
has issue date	2020-04-11(xsd:dateTime)
bibo:doi	10.1101/2020.04.08.20058495
has license	medrxiv
sha1sum (hex)	12c27668d532b86334400132f83b995a7df41b2d
schema:url	https://doi.org/10.1101/2020.04.08.20058495
resource representing a document's title	Fast SARS-CoV-2 detection by RT-qPCR in preheated nasopharyngeal swab samples
resource representing a document's body	covid:12c27668d532b86334400132f83b995a7df41b2d#body_text
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